Camellia meal polypeptides, preparation method and application in promoting oestrus and conception of anestrus cows
A technology of camellia oleifera meal polypeptide and camellia oleifera meal, which is applied in the field of animal husbandry, can solve the problems such as the in-depth development of hydrolyzed polypeptide of small fruit camellia oleifera meal, and achieve the effects of reusing waste, promoting estrus and conception, and being easy to implement
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Embodiment 1
[0046] Example 1: Preparation of camellia oleifera meal polypeptide 1 by trypsin
[0047] Including the following steps:
[0048] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 150g / L;
[0049] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 3.0g / 100g based on the weight of meal powder), the enzymatic hydrolysis conditions are: pH value, 8.0; enzymatic hydrolysis temperature, 45°C; enzymatic hydrolysis time, 11 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;
[0050] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;
[0051] Step S4, dissolve the crude freeze-dried powder with ult...
Embodiment 2
[0052] Example 2: Preparation of camellia oleifera meal polypeptide 2 by trypsin
[0053] Including the following steps:
[0054] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 130g / L;
[0055] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 2.5g / 100g based on the weight of meal flour), the enzymatic hydrolysis conditions are: pH value, 7.8; enzymatic hydrolysis temperature, 43°C; enzymatic hydrolysis time, 12 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;
[0056] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;
[0057] Step S4, dissolve the crude freeze-dried powder with ultr...
Embodiment 3
[0058] Example 3: Preparation of camellia oleifera meal polypeptide 3 by trypsin
[0059] Including the following steps:
[0060]Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 170g / L;
[0061] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 3.5g / 100g based on the weight of meal powder), the enzymatic hydrolysis conditions are: pH value, 8.2; enzymatic hydrolysis temperature, 47°C; enzymatic hydrolysis time, 10 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;
[0062] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;
[0063] Step S4, dissolve the crude freeze-dried powder with ultr...
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