The production process of abamectin

Abstract

The invention discloses a production process of abamectin, comprising the steps of shaking flask seed cultivation, seed tank cultivation, and fermenter tank cultivation, seed culture medium is used in the shaking flask seed cultivation and seed tank cultivation steps, and the The carbon source of the seed culture medium is glucose; the fermented medium is used in the fermentor culture step, and the carbon source of the fermented medium is glucose and sorbitol; the carbon source is supplemented in the fed-batch mode in the fermentor culture step , the carbon source is glucose and sorbitol. The obtained production process can effectively improve the dissolved oxygen in the fermentation process, reduce the power cost of production; and avoid the feedback inhibition of microbial metabolism caused by adding too much glucose, so as to ensure the improvement of the fermentation unit when putting the tank; it can also reduce the post-fermentation The sugar removal step in the extraction stage significantly reduces the COD of the wastewater produced by fermentation.

Description

technical field [0001] The invention relates to the technical field of abamectin fermentation production, in particular to a production process of abamectin. Background technique [0002] Avermectins are a group of 16-membered macrolide antibiotics with insecticidal, acaricidal and nematicidal activities, which are fermented by Streptomyces avermitilis in Streptomyces griseus. Natural abamectin contains 8 homologue components with similar structures, the main 4 species are A1a, A2a, B1a and B2a, and their total content is ≥80%; the corresponding 4 homologues with smaller proportions are A1b, A2b, B1b and B2b, the total content of which is ≤20%. Abamectin is widely used in agriculture and is a common pesticide preparation. It is used as an insecticide to control Brassicaceae diamondback moth, cotton bollworm, mites, cabbage caterpillar, etc. [0003] At present, the basal medium used for fermentation and production of abamectin uses high-concentration starch as the carbon s...

AI Technical Summary

Problems solved by technology

However, due to the viscous starch feed liquid, the effect of dissolved oxygen in the medium will be poor. Generally, under the requirement of an average level of 6g / L for the production tank unit, the tank release coefficient is generally lower than 75%; and the starch can only be converted into glucose first. It can be used by microorganisms, but other sugars will be produced during the fermentation process, which not only makes the utilization rate of starch low, but also needs to add toluene to remove various sugars produced by starch metabolism in the extraction process after fermentation, so that the fermentation produces The wastewater COD is very high, which puts pressure on environmental protection

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