Streptomyces deccanensis QY-3 and application thereof
A technology of Streptomyces decanata, QY-3, applied in the application, bacteria, fungicides and other directions, to achieve the effect of broad-spectrum anti-phytopathogenic fungi activity and good control effect
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Embodiment 1
[0026] Example 1 Isolation and screening of Streptomyces deccanensis QY-3
[0027] Take glacier surface soil to obtain glacier sediment. Take 10g of glacier sediment sample, grind it fully, put it into a triangular flask with 100mL sterile water and glass beads inside, vibrate on a shaker at 100rpm for 20min, take the supernatant for 10 times Gradual dilution to obtain the dilution, take 0.1mL of the dilution and spread it evenly on the Gaussian No. 1 medium plate, place it in a constant temperature incubator at 28°C and cultivate it for 5-10 days, pick up actinomycetes colonies and further separate them by streaking Purify until the pure culture of actinomycete strains is obtained; with G. anthracnose as the target bacteria, a strain QY-3 with high inhibitory activity against G. anthracnose was screened by confrontation culture method.
[0028] The biological characteristics of Streptomyces deccanensis QY-3 of the present invention are as follows:
[0029] The effective frag...
Embodiment 2
[0042] Embodiment 2: the inhibitory activity determination of Streptomyces deccan QY-3 to pathogenic bacteria
[0043] Fermentation medium used in the present invention: millet 20g, peptone 3g, glucose 20g, CaCO 3 2g, NaCl 2.5g, water 1000mL, pH: 7.2-7.4, culture conditions are 28°C, 180rpm shaking culture for 7d.
[0044] The inhibitory activity of Streptomyces deccan QY-3 against G. anthracnose was determined by plate confrontation test. As shown in Figure 2, Streptomyces deccan QY-3 had strong antagonistic activity against G. anthracnose.
[0045] Streptomyces deccan QY-3 was fermented and cultured, and the supernatant of the fermentation broth was obtained by filtering out the bacteria, and its inhibitory activity against different plant pathogenic fungi was tested. As can be seen from Table 4, the fermented liquid of Streptomyces deccan QY-3 has broad-spectrum antifungal activity, especially to the pathogenic bacteria of the genus Anthracnose. 87.0% and 88.9%, respecti...
Embodiment 3
[0049] Embodiment 3: the temperature and pH stability determination of Streptomyces deccan QY-3 fermented liquid
[0050] Streptomyces deccan QY-3 fermentation broth was treated at different temperatures and pHs for 1 hour, and its inhibitory activity against G. anthracnose was determined. Depend on image 3 Shown, Streptomyces deccan QY-3 fermentation broth has better temperature and pH stability.
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