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Acyl-homoserine lactone degrading bacteria and application of acyl-homoserine lactone degrading bacteria in disease control

A strain and bacillus technology, applied in the field of acyl homoserine lactone degrading bacteria and its application in disease control, can solve the problems of acyl side chain length, saturation difference, etc.

Active Publication Date: 2018-05-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Among them, N-Acyl homoserine lactones (N-Acyl homoserine lactones, AHLs) are the unique quorum sensing signals of Gram-negative bacteria. Most of these substances have the same homoserine lactone ring structure, and all have acyl Side chains, but there are differences in the length and degree of saturation of the acyl side chains

Method used

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  • Acyl-homoserine lactone degrading bacteria and application of acyl-homoserine lactone degrading bacteria in disease control
  • Acyl-homoserine lactone degrading bacteria and application of acyl-homoserine lactone degrading bacteria in disease control
  • Acyl-homoserine lactone degrading bacteria and application of acyl-homoserine lactone degrading bacteria in disease control

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Embodiment 1

[0044] Isolation and screening of embodiment 1OdDHL degrading bacteria (Acinetobacter bacterial strain XJ-10)

[0045] 1. Isolation and screening of Acinetobacter strain XJ-10

[0046] (1) Soil sample collection: Soil samples collected from Xinjiang were used as microbial sources.

[0047] The soil samples were collected from the farmland of Xinjiang Province on October 9, 2014. They were sampled, bagged and preserved as microbial sources and brought back to the laboratory for isolation and screening of strains.

[0048] (2) Enrichment culture of bacterial strains: prepare basal salt medium (MSM), put 50mL of MSM medium in a 250mL Erlenmeyer flask, sterilize at 121°C for 15-25min, add OdDHL mother liquor (solvent Methanol), so that the final concentration of OdDHL is 5 μM, in addition to adding 5 g of soil samples, placed in 30 ° C, 200 rpm shaker for 7 days, and then transferred to MSM medium with OdDHL concentration of 10 μM according to 10% inoculum . After culturing for...

Embodiment 2

[0054] The identification of embodiment 2 Acinetobacter strain XJ-10

[0055] In this example, the morphological characteristics, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis of the degrading bacteria XJ-10 were carried out, and the strain was identified as Acinetobacter schindleri. details as follows:

[0056] (1) Morphological characteristics of the colonies: After culturing on LB solid plates for 48 hours, the colonies are flat, with smooth and opaque surfaces and neat edges, such as figure 1 Shown; the bacterial colony appears beige on the blood plate, as figure 2 Shown; in LB liquid medium, it is diffuse and turbid, aerobic, and grows well at 30°C.

[0057] (2) Morphological characteristics of bacteria: such as image 3 As shown, the bacteria are rod-shaped, sometimes nearly spherical, with one polar flagella.

[0058] (3) Physiological and biochemical characteristics: the strain is Gram-negative bacteria, aerobic, positive in cont...

Embodiment 3

[0069] The antibiotic susceptibility analysis of embodiment 3 strain XJ-10

[0070] In order to better study the strain XJ-10, various antibiotic susceptibility tests were performed on the strain XJ-10 in this example.

[0071] Analysis of experimental results: such as Figure 5 As shown, the resistance of the strain XJ-10 to ampicillin reached 400 μg / mL and above, the resistance to kanamycin reached 200 μg / mL, the resistance to gentamicin reached 100 μg / mL, and the resistance to streptomycin The resistance to chloramphenicol and chloramphenicol reached 20 μg / mL. This result is helpful for selecting appropriate antibiotics as a reference in follow-up studies.

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Abstract

The invention discloses acyl-homoserine lactone degrading bacteria and application of the acyl-homoserine lactone degrading bacteria in disease control. The degrading bacteria are Acinetobacter schindleri and particularly refer to an acinetobacter strain XJ-10, the strain is collected in the China Center for Type Culture Collection on 2nd, November, 2017, and the collection number is CCTCC NO:M2017650. The strain can degrade quorum sensing signal molecules, is used for unique carbon source growth, and has a remarkable and rapid degradation effect on the AHLs quorum sensing signal molecules. Thus, microbial quorum sensing communication can be blocked through degradation of the quorum sensing signal molecules, expression of pathogenic factors of pathogenic bacteria is restrained, the purposeof disease control is achieved, and great application potential is achieved in the aspect of controlling the harm of pathogenic bacteria depending on AHLs mediated pathopoiesis. The problem of abuseof chemical pesticide can be solved, and meanwhile a novel strategy is provided for biologically controlling phytopathogen harm.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to an acyl homoserine lactone degrading bacterium and its application in disease control. Background technique [0002] Microorganisms sense changes in population density by monitoring the concentration of extracellular signaling molecules (autoinducers), and when they reach a certain threshold, they start the expression of target genes and coordinate group behavior, that is, quorum sensing (Quorum Sensing, QS) (Whiteley M, Diggle S P, Greenberg E P. Progress in and promise of bacterial quorum sensing research [J]. Nature. 2017, 551(7680): 313-320.). Quorum sensing widely exists in microbial populations and is related to various biological functions of microorganisms, such as: bioluminescence, symbiosis, biofilm formation, antibiotic synthesis, population mobility, plasmid transfer, spore formation, gene exchange, etc. (Davies DG, Parsek MR, Pearson JP, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 陈少华范兴辉张炼辉叶田王惠杉郭云帆张译尹
Owner SOUTH CHINA AGRI UNIV
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