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A kind of composite bacteria agent for foreign aid nutrition of hickory chick cultivation and its application

A technology of morel cultivation and compound bacterial agent, which is applied in the field of compound bacterial agent and the nutrient material produced by using it, can solve the problems of yield impact, etc., and achieve the effect of low cost, good effect, simple production process and use method

Active Publication Date: 2021-06-18
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the commercial cultivation stage of Morchella, the cultivation without the use of foreign aid nutrition basically ended in failure. The lack of foreign aid nutrition bags under the current technical system has a great impact on the yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 A kind of hickory chick cultivation foreign aid nutritional material

[0024] (1) Compound bacterial agent

[0025] PDA slant medium: The medium formula is: soluble starch 15g, yeast extract 5g, peptone 3g, potassium nitrate 1g, manganese sulfate 5mg / L, sodium molybdate 6mg / L, dipotassium hydrogen phosphate 6g, sodium chloride 3g , magnesium sulfate 4 g, agar 20 g, water 1000 ml, adjust the pH value to 7.2. Sterilize at 121 °C for 30 min, and then distribute it into petri dishes.

[0026] Cellulomonas chrysogenum, Candida utilis and Geotrichum candidum were inoculated on the slant medium, and cultured at a constant temperature at 38°C for 24 hours. Microscopic examination showed no bacterial infection, and the spore rate was greater than 90%. Store the cultured strains below 3°C.

[0027] Liquid medium: The medium formula is: soluble starch 15g, yeast extract 5g, peptone 3g, potassium nitrate 1g, manganese sulfate 1mg / L, sodium molybdate 3mg / L, dipotassiu...

Embodiment 2

[0037] Embodiment 2 A kind of hickory chick cultivation foreign aid nutritional material

[0038] (1) Compound bacterial agent

[0039]PDA slant medium: The medium formula is: soluble starch 8g, yeast extract 3g, peptone 2g, potassium nitrate 2g, manganese sulfate 1mg / L, sodium molybdate 3mg / L, dipotassium hydrogen phosphate 3g, sodium chloride 5g , magnesium sulfate 1.5 g, agar 20 g, water 1000 ml, adjust the pH value to 7.3. The culture medium was sterilized at 121°C for 30 minutes, and then distributed into Petri dishes.

[0040] Cellulomonas chrysogenum, Candida utilis and Geotrichum candidum were inoculated on the slant medium, and cultured at a constant temperature at 38°C for 24 hours. Microscopic examination showed no bacterial infection, and the spore rate was greater than 90%. Store the cultured strains below 3°C.

[0041] Liquid medium: The medium formula is: soluble starch 15g, yeast extract 5g, peptone 3g, potassium nitrate 1g, manganese sulfate 1mg / L, sodium m...

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PUM

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Abstract

The invention discloses a compound bacterial agent for foreign aid nutrition in hickory chick cultivation and an application thereof, belonging to the field of edible fungus cultivation. The compound microbial agent used in the nutrition material of the present invention includes Cellulomonas lutea, Candida utilis and Geotrichum candidum. According to the formula of 30-35 parts of rice husk, 30-35 parts of straw, 30-35 parts of fungus chaff, 1-2 parts of gypsum, 2-3 parts of glucose, 1-2 parts of table salt and 0.1-0.3 parts of potassium dihydrogen phosphate The compound microbial agent is fermented and put into a plastic bacterial bag. The same side of the material bag is eye-catching, with a hole diameter of 1-2cm. Place 2 rows on both sides of the morel mushroom bed, and the opening is tightly covered with soil. The compound microbial agent of the present invention is symbiotically coordinated, does not antagonize each other, and can provide sufficient foreign aid nutrition after being fermented with a substrate. The invention uses the composite bacterial agent and the bacterial chaff rich in growth-promoting substances as external nutrition, which can significantly increase the yield and accelerate the germination speed of the hickory chick.

Description

technical field [0001] The invention belongs to the fields of edible fungus cultivation and microorganism utilization, and in particular relates to a compound bacterial agent for foreign aid nutrition in morel cultivation and a nutrient material made by using the same. Background technique [0002] Morchella [Morehella esculenta (L.) Pers] is a rare edible and medicinal fungus, which was discovered in 1818 and is named for its shape like morel. The nutritional content of morel is comparable to that of milk, meat and fish meal. Therefore, it is often called one of the "healthy foods" internationally. Morchella also has the functions of benefiting the stomach, digestion and food, reducing phlegm and regulating qi, nourishing the kidney, strengthening yang, nourishing the brain, and refreshing. There is a folk saying that "eat morel every year, and walk all over the mountains at 80". [0003] The earliest record about the cultivation of morels was in 1883, and there were man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C05G1/00A01G18/20C12R1/01C12R1/72C12R1/645
CPCC05B7/00C12N1/14C12N1/20C05D3/00C05D9/00C05F5/00C05F5/002C05F11/00C05F11/08
Inventor 李超李红杨镇刘国宇何雨张敏李学龙曹君刘国丽
Owner LIAONING ACAD OF AGRI SCI
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