Method for measuring zilpaterol by using CdTe quantum dot

A technology of quantum dots and concentration, which is applied in the field of determination of zilpaterol by using CdTe quantum dots, which can solve problems such as the inability to accurately and effectively detect the content of zilpaterol

Active Publication Date: 2018-05-15
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the inability to accurately and effectively detect the zilpaterol content in the sample in the prior art, thereby providing a specificity, high sensitivity, and low cost detection agent using CdTe quantum dots. Paltrow's method

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  • Method for measuring zilpaterol by using CdTe quantum dot
  • Method for measuring zilpaterol by using CdTe quantum dot
  • Method for measuring zilpaterol by using CdTe quantum dot

Examples

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Embodiment 1

[0071] The present embodiment provides a kind of method utilizing CdTe quantum dot to measure zilpaterol, comprises the following steps:

[0072] S1. Take 0.2 mL of zilpaterol antibody dilution solution with a concentration of 0.5 mg / mL, add 50 μL of EDC with a concentration of 0.10 mol / L and 50 μL of NHS with a concentration of 0.10 mol / L, leave it at room temperature for 15 minutes, and add 0.10 mL of EDC with a concentration of 0.10 mol / L. 1.25×10 -4 mol / L CdTe quantum dot solution with carboxyl groups on the surface, use pH=7.4 PBS buffer solution (containing 0.4mol / L NaCl and Tween-20 with a mass concentration of 0.05%) to make up to 5.0mL, and react at 4°C 48h, get CdTe-zipaterol antibody, spare;

[0073] S2. Use CBS buffer solution with a concentration of 0.05 mol / L and pH=9.6 as the coating medium, dilute the zilpaterol antigen to a concentration of 45 μg / mL, add to the wells of the microplate, 100 μL per well, 4°C After reacting for 10 h, wash with PBS buffer soluti...

Embodiment 2

[0078] The present embodiment provides a kind of method utilizing CdTe quantum dot to measure zilpaterol, comprises the following steps:

[0079] S1. Take 0.1 mL of zilpaterol antibody at a concentration of 0.3 mg / mL, add 45 μL of EDC with a concentration of 0.08 mol / L and 45 μL of NHS with a concentration of 0.08 mol / L, leave it at room temperature for 12 minutes, and add 0.2 mL of a concentration of 1 ×10 -4mol / L of CdTe quantum dots with carboxyl groups on the surface, use pH=6.5 PBS buffer solution (containing 1.0 mol / L NaCl and Tween-20 with a mass concentration of 0.01%) to make up to 5.0mL, and react at 4°C for 46h, Obtain CdTe-zipaterol antibody, spare;

[0080] S2. Use PBS buffer solution with a concentration of 0.01mol / L and pH=7.4 as the coating medium, dilute the zilpaterol antigen to a concentration of 40 μg / mL, add to the wells of the microplate, 100 μL per well, 4 °C After reacting for 5 h, wash twice (5 min each time) with PBS buffer solution (containing 1.0 ...

Embodiment 3

[0085] The present embodiment provides a kind of method utilizing CdTe quantum dot to measure zilpaterol, comprises the following steps:

[0086] S1. Take 0.3 mL of zilpaterol antibody at a concentration of 0.7 mg / mL, add 55 μL of EDC with a concentration of 0.12 mol / L and 55 μL of NHS with a concentration of 0.12 mol / L, leave it at room temperature for 18 minutes, add 0.1 mL of EDC with a concentration of 1.5 ×10 -4 mol / L CdTe quantum dots with carboxyl groups on the surface, dilute to 5.0mL with pH=7.8 PBS buffer solution (containing 0.2mol / L NaCl and 0.1% Tween-20), and react at 4°C for 50h , get CdTe-zipaterol antibody, spare;

[0087] S2. Use Tris-HCl buffer solution with a concentration of 0.01mol / L and pH=8.0 as the coating medium, dilute the zilpaterol antigen to a concentration of 55 μg / mL, add it to the wells of the microtiter plate, 100 μL per well, React at 4°C for 12 h, wash with PBS buffer solution (containing 0.2 mol / L NaCl and 0.1% Tween-20 at a mass concentr...

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Abstract

The invention discloses a method for measuring zilpaterol by using a CdTe quantum dot. The method comprises the following steps: preparing a CdTe-zilpaterol antibody; preparing a zilpaterol antigen-coated ELISA plate; adding zilpaterol standard solutions and CdTe-zilpaterol antibody solutions with a series of concentration into each hole of the ELISA plate, setting a blank control group simultaneously, incubating and washing; measuring the fluorescence intensity F of each hole in the ELISA plate, setting the fluorescence intensity of the blank control group as F0, calculating the inhibition rate I which is equal to (F-F0)/(Fmax-F0), and drawing a standard curve by taking the inhibition rate as vertical coordinate and the logarithm of the zilpaterol standard solution concentration as horizontal coordinate; and adding a to-be-detected sample and the CdTe-zilpaterol antibody solution into the ELISA plate, incubating, washing, measuring the fluorescence intensity and substituting into thestandard curve to obtain the zilpaterol concentration of the to-be-detected sample. According to the method, the zilpaterol is measured by the CdTe quantum dot, the detection cost is low, the detection time is short, the sensitivity is high and the specificity is high.

Description

technical field [0001] The invention belongs to the field of quantitative detection of zilpaterol, in particular to a method for measuring zilpaterol by utilizing CdTe quantum dots. Background technique [0002] Zipaterol, a new type of β-receptor agonist, is a growth promoter that has the same physiological effects as clenbuterol (commonly known as clenbuterol), ractopamine, albuterol, etc. It is a prohibited drug, but Its chemical structure is different from common β-receptor agonists, and its toxicity is 15 times that of ractopamine. It can transfer the nutrients in animals from fat to muscle, showing a nutrient redistribution effect, and then regulates the substances in animals. Metabolism, enhance fat decomposition, promote protein synthesis, and significantly enhance leptin in ketone bodies, which can cause human or animal muscle tremors, heart failure, and even death, causing harm to the health and life safety of the people. [0003] Currently the main detection meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 魏益华张金艳罗林广李瑞丽赖艳
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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