Antibacterial insole preparation method
A technology of insoles and seeds, which is applied in the field of daily necessities, can solve the problems of unsustainable antibacterial effect, poor deodorant effect, and poor air permeability of insoles, and achieve the effects of strong air permeability, high water holding capacity, and strong biocompatibility
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[0023] A preparation method for an antibacterial insole, comprising the steps of:
[0024] (1) According to the number of parts by mass, take 2~5 parts of Angelica sinensis, 3~6 parts of Shenjincao, 5~6 parts of Sichuan Achyranthes bidentata, 2~6 parts of Salvia miltiorrhiza, 3~7 parts of Muxiang, 2~6 parts of Lycium barbarum Mix, wash, and crush through a 100-mesh sieve, collect the sieved particles, conduct steam explosion at 0.35MPa for 110~120s, collect the blasting particles, add 70% ethanol solution at a mass ratio of 1:8, and keep at 25~30°C Extract for 1~2h, filter, take the filtrate to rotary evaporate, and concentrate under reduced pressure to obtain the nutrient concentrate;
[0025] (2) Take Acetobacter xylinum and inoculate it into the seed culture solution at a mass ratio of 1:10, and culture it at 150 rpm at 28-30°C for 1-2 days to obtain the seed culture solution. Take the seed culture solution and inoculate it at a 10% inoculation amount until fermentation cu...
Embodiment 1
[0029] Acetobacter xylinum: refrigerated storage on inclined planes.
[0030] Seed medium: in parts by mass, take 18 parts of glucose, 5 parts of yeast extract, 1 part of potassium dihydrogen phosphate, 12 parts of magnesium sulfate heptahydrate, 1000 parts of water, pH 6.8, sterilize at 121°C for 20 min, cool, Take the sterilized substance and add absolute ethanol at a mass ratio of 1:14 to obtain it.
[0031]Fermentation medium: in parts by mass, take 20 parts of glucose, 5 parts of yeast extract, 1 part of potassium dihydrogen phosphate, 15 parts of magnesium sulfate heptahydrate, 1000 parts of water, pH 6.8, and sterilize at 121°C for 20 minutes.
[0032] Polyester fiber non-woven fabric: purchased from Hangzhou Luxian Non-woven Co., Ltd.
[0033] A preparation method for an antibacterial insole, comprising the steps of:
[0034] (1) According to the number of parts by mass, take 2 parts of Angelica sinensis, 3 parts of Shenjincao, 5 parts of Sichuan Achyranthes bidentat...
Embodiment 2
[0039] Acetobacter xylinum: refrigerated storage on inclined planes.
[0040] Seed medium: in parts by mass, take 22 parts of glucose, 8 parts of yeast extract, 2 parts of potassium dihydrogen phosphate, 15 parts of magnesium sulfate heptahydrate, 1000 parts of water, pH 7.0, sterilize at 121°C for 20 minutes, cool, take The sterilized product is obtained by adding absolute ethanol at a mass ratio of 1:14.
[0041] Fermentation medium: in parts by mass, take 25 parts of glucose, 7 parts of yeast extract, 2 parts of potassium dihydrogen phosphate, 18 parts of magnesium sulfate heptahydrate, 1000 parts of water, pH 7.0, and sterilize at 121°C for 20 minutes.
[0042] Polyester fiber non-woven fabric: purchased from Hangzhou Luxian Non-woven Co., Ltd.
[0043] A preparation method for an antibacterial insole, comprising the steps of:
[0044] (1) According to the number of parts by mass, take 5 parts of Angelica sinensis, 6 parts of Shenjincao, 6 parts of Sichuan Achyranthes bi...
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