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Preparation method of oudemansiella raphanipes liquid strain

A technology of liquid strains and chicken fir fungus, which is applied in botany equipment and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low yield and slow growth of mycelium, and achieve fast growth , cell activity, and the effect of improving stability

Inactive Publication Date: 2018-05-15
四川山水美地农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the problems existing in the prior art, the object of the present invention is to provide a kind of method for preparing the liquid bacterial strain of P. nigriscens that can promote the production speed of mycelium, to solve the mycelium of P. nigris artificial culture process in the prior art Problems of slow growth and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of preparation method of chicken fruticosa liquid strain, comprises the following steps:

[0023] Step 1): Medium preparation: 350 grams of potato juice, 150 grams of grape juice, 40 grams of agar, 0.7 grams of magnesium sulfate, 5 grams of mulberry fermentation powder and 454.3 grams of water;

[0024] The potato juice is obtained by mashing fresh and clean potatoes, twisting and filtering. The grape juice is obtained by squeezing fresh clean grape juice and filtering it. The mulberry fermented powder is obtained by adding mulberry fruit to brown sugar water, sealing and fermenting at 25° C. for 5 months, filtering, concentrating and drying. The mass ratio of the mulberry fruit to the brown sugar water is 1:4, and the brown sugar water is obtained by dissolving brown sugar in water with 3 times the mass.

[0025] Step 2): Sterilize the medium: filter the medium obtained in step 1), place it in a sterilizer at 120°C for 45 minutes, and cool it to 25°C in a ster...

Embodiment 2

[0030] A kind of preparation method of chicken fruticosa liquid strain, comprises the following steps:

[0031] Step 1): Medium preparation: 400 grams of potato juice, 160 grams of grape juice, 35 grams of agar, 0.8 grams of magnesium sulfate, 4.2 grams of mulberry fermentation powder and 400 grams of water;

[0032] The potato juice is obtained by mashing fresh and clean potatoes, twisting and filtering. The grape juice is obtained by squeezing fresh clean grape juice and filtering it. The mulberry fermented powder is obtained by adding mulberry fruit to brown sugar water, sealing and fermenting at 24° C. for 6 months, filtering, concentrating and drying. The mass ratio of the mulberry fruit to the brown sugar water is 1:4, and the brown sugar water is obtained by dissolving brown sugar in water with 3 times the mass.

[0033] Step 2): medium sterilization: filter the medium obtained in step 1), place it in a sterilizer at 120°C for 45 minutes, and cool it to 24°C in a ster...

Embodiment 3

[0038] A kind of preparation method of chicken fruticosa liquid strain, comprises the following steps:

[0039] Step 1): Medium preparation: 380 grams of potato juice, 140 grams of grape juice, 38 grams of agar, 0.5 grams of magnesium sulfate, 4.5 grams of mulberry fermentation powder and 437 grams of water;

[0040] The potato juice is obtained by mashing fresh and clean potatoes, twisting and filtering. The grape juice is obtained by squeezing fresh clean grape juice and filtering it. The mulberry fermented powder is obtained by adding mulberry fruit to brown sugar water, sealing and fermenting at 24° C. for 6 months, filtering, concentrating and drying. The mass ratio of the mulberry fruit to the brown sugar water is 1:4, and the brown sugar water is obtained by dissolving brown sugar in water with 3 times the mass.

[0041] Step 2): Sterilize the medium: filter the medium obtained in step 1), place it in a sterilizer at 120°C for 45 minutes, and cool it to 24°C in a ster...

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Abstract

A preparation method of an oudemansiella raphanipes liquid strain comprises the steps that firstly, a culture medium is prepared, wherein 1 kg of the culture medium serves as a benchmark, the culturemedium contains 300-400g of potato juice, 100-200 g of grape juice, 30-50 g of agar, 0.5-1 g of magnesium sulfate, 3-6 g of paper mulberry fermented powder and balance water; secondly, the culture medium is sterilized, wherein after being filtered, the culture medium obtained in the first step is placed in a sterilization device to be sterilized, and then is cooled after sterilization; thirdly, astrain is inoculated, wherein oudemansiella raphanipes mycelia are inoculated in the culture medium obtained in the second step, and then the culture medium is placed on a shaking bed for oscillationculture; fourthly, fermentation culture is conducted, wherein the liquid strain obtained after oscillation culture in the third step is placed in a fermentation tank for fermentation culture. According to the method, the liquid culture medium prepared from the potato juice, the grape juice, the agar, the magnesium sulfate and the paper mulberry fermented powder is used, the components work together to provide various nutrients for growth of the mycelia, the growth speed of the mycelia can be high, the biomass of the mycelia is 2.3 g / ml or above, and the mycelia are thick and a little amber.

Description

technical field [0001] The invention belongs to the field of fungus cultivation, and in particular relates to a method for preparing a liquid strain of Gallus pilosulae. Background technique [0002] Gallopia black skin belongs to the order Agaricaceae, and it is a kind of wild edible fungus that grows in summer and autumn in temperate and subtropical regions. Because its growth and development are closely related to the activities of soil termites, and it is highly dependent on the growth area, the artificial cultivation requires high and difficult requirements. And because of the tender meat, strong smell and delicious taste, it is one of the famous wild edible mushrooms, and the taste of the black-skinned mushroom is the best. Traditional Chinese medicine believes that the fungus is flat and sweet, has the effects of invigorating the spleen and Qi, appetizing and refreshing, and relieving pain and edema. It is an ideal food for treating hemorrhoids. Modern medicine has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20C12N1/14C12R1/645
CPCC12N1/14
Inventor 吴玲周善龙
Owner 四川山水美地农业投资有限公司
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