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Culture medium for fermenting demeclocycline and fermentation method of demeclocycline

A demethylaureomycin and culture medium technology, which is applied in the field of fermentation medium for demethylaureomycin, can solve the problems of low fermentation titer and residual toxic substances, and achieve increased fermentation yield, stable chemical properties, and reduced costs Effect

Active Publication Date: 2018-05-11
SHANGHAI INST OF PHARMA IND +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to provide a new method for the deficiencies of residual toxic substances and low fermentation titer in the fermentation liquid of demethylaureomycin obtained by fermentation of Streptomyces aureomycin in the existing A medium and a fermentation method for fermenting demethylaureomycin, which can increase the fermentation unit of demethylaureomycin and improve production efficiency

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  • Culture medium for fermenting demeclocycline and fermentation method of demeclocycline
  • Culture medium for fermenting demeclocycline and fermentation method of demeclocycline
  • Culture medium for fermenting demeclocycline and fermentation method of demeclocycline

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Embodiment 1

[0035]Under sterile conditions, a strain of Streptomyces aureofaciens NRRL3203 (purchased from the American Agricultural Research Culture Collection) was inoculated into the slant medium. After 2 days of slant culture at 28°C, light yellow-brown bacteria can be seen on the surface of the medium; after 3 or 4 days, the color of the medium deepens, and a small amount of white spores are formed; after 7-8 days, a large number of white spores can be seen on the surface of the medium, and the medium The matrix itself turns dark brown due to the production of Streptomyces aureomycin pigment. Under sterile conditions, use a sterilized inoculation shovel to gently scratch the sloping medium with spores, dig out as thin as possible a 0.8cm×1.5cm sloping medium full of spores, and inoculate the sterilized seeds In the culture medium, cultured on a rotary shaker at 28°C and 240rpm for 40-46h, it can be observed that the color of the culture in the shake flask changes from light yellow to...

Embodiment 2

[0038] (1) Single carbon source determination experiment

[0039] Based on the formula of the basic fermentation medium, the nitrogen source, inorganic salt and other components and contents remained unchanged, and corn starch, glucose, sucrose, fructose, lactose, maltose, dextrin, mannitol, etc. were added to conduct single-factor carbon source experiments. The dosage is 8%. The fermented seeds prepared in Example 1 are inoculated in the fermentation medium with 10% (v / v) inoculum, pH7.0, loading 35mL / 250mL, at 30°C, 240rpm rotary shaker The bed culture was cultivated for 8 days, and the fermentation titer was measured by high performance liquid phase.

[0040] Basic fermentation medium composition: 8.0% corn starch, 3.0% soybean flour, 0.8% L-lysine, 0.3% sodium chloride, 0.2% ammonium sulfate, 1.0% calcium carbonate, 0.3% corn steep liquor, 0.6% soybean oil, α - Amylase 0.08%, deionized water 100 mL. The fermentation titer obtained by using the basic fermentation medium f...

Embodiment 3

[0049] (1) Nitrogen source determination experiment

[0050] According to the results of the above experiments, the 8% cornstarch in the basal medium was added to 11%, taking the optimized medium formula as a control, and under the condition of keeping other ingredients such as inorganic salts unchanged, fish meal, cottonseed meal, feather soybean flour, peanut flour, soybean meal powder or milk powder were all substituted for soybean flour, and the amount of replacement nitrogen source was 3%, and the shake flask was fermented for 8 days, and the fermentation titer was measured by high performance liquid phase. Experimental results show that: under the same culture conditions, compared with other nitrogen sources, when the nitrogen source is soybean flour, the titer of the fermented liquid is the highest.

[0051] Optimized medium: 11% corn starch, 3.0% soybean flour, 1.0% calcium carbonate, 0.8% L-lysine, 0.3% sodium chloride, 0.3% ammonium sulfate, 0.3% corn steep liquor, 0...

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Abstract

The invention discloses a culture medium for fermenting demeclocycline and a fermentation method of the demeclocycline. The culture medium for fermenting the demeclocycline includes a carbon source, anitrogen source, amino acids, inorganic salts, alpha-amylase, vegetable oil and water, the content of the carbon source is 9-13%, and the content of the nitrogen source is 2.8-4.0%. Through adjustingof the content of the carbon source and the nitrogen source, especially the replacement of the nitrogen source and the addition of trace elements, the fermentation yield of the demeclocycline is improved. The provided fermentation method of the demeclocycline significantly increases the fermentation unit of the demeclocycline, and is suitable for the large-scale production of the demeclocycline.

Description

technical field [0001] The invention belongs to the technical field of industrial microbes, and in particular relates to a culture medium and a fermentation method for fermenting desmethylaureomycin. Background technique [0002] Demethylchlortetracycline is a better broad-spectrum antibiotic. Its antibacterial activity against most bacteria is about twice that of tetracycline. The human body absorbs it better than tetracycline and retains it in the body for a long time. The dimethylaminotetracycline synthesized with it as the main raw material has the characteristics of high efficiency, quick-acting, long-acting, etc., low toxicity and small side effects, and is considered to be one of the best varieties among the existing tetracycline antibiotics. At present, Streptomyces aureus is mainly used to ferment and produce desmethylaureomycin, wherein the selection of the components of the medium used for fermentation and the determination of their content are important factors a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P29/00C12R1/485
CPCC12P29/00
Inventor 李继安林惠敏汪玉真夏爱坤徐鲁刘坤宋乐何强张建斌李亚军
Owner SHANGHAI INST OF PHARMA IND
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