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Preparation method and application of a gene-loaded multifunctional contrast agent

A multi-functional, contrast agent technology, applied in the field of ultrasound imaging, can solve the problems of ultrasonic-triggered microsphere phase transition difficulties, adverse biological effects, etc., and achieve the effect of regular morphology and good stability

Active Publication Date: 2021-04-16
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of Laplace pressure and surface stabilizers makes it relatively difficult for ultrasound to trigger the phase transition of microspheres. Therefore, the pressure and frequency of traditional diagnostic ultrasound are not enough to stimulate the phase transition of microspheres, and the ultrasonic pressure and frequency may also cause adverse biological effects

Method used

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  • Preparation method and application of a gene-loaded multifunctional contrast agent
  • Preparation method and application of a gene-loaded multifunctional contrast agent
  • Preparation method and application of a gene-loaded multifunctional contrast agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039]First, a multifunctional contrast agent (FA-CN-PFP-ICG / PDNA, referred to as FA-CN-PFP-ICG / PDNA), which is:

[0040]1), dissolve:

[0041]DPPC (Dippocyanoyl choline, USA Avanti), DSPE- (PEG2000) -FOLATE (modified polyethylene glycol - (2000) - Dihalikanoyl ethalamine, USA Avanti Company), DC -cholesterol (DC-cholesterol, USA Avanti) A total of 10 mg is added to the round bottom flask according to the mass ratio of 5: 2: 2, and at the same time, 6 ml of trichloromethane is added to the round bottom flask, and the round bottom flask is closed. Heating in ° C is heated to sufficient dissolution.

[0042]2), rotary evaporation: After dissolving 20 minutes, fix the round bottom flask on the rotary evaporator (temperature: 50 ° C) to rotate evaporation to remove organic solvent, rotational speed: 80 rpm, time: 2 hours, form a uniform white film.

[0043]3), ultrasonic cleaning: After the end of the evaporation, remove the round bottom flask, add 6 mL of PBS oscillation, water, and then place...

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Abstract

The invention discloses a gene-carrying multifunctional contrast agent, which comprises a lipid shell membrane modified with folic acid, the surface of the lipid shell membrane is positively charged and carries genes. The technical problem to be solved by the present invention is to provide a kind of gene-carrying drug that has the ability to target in vivo and in vitro, has the ability to enhance ultrasound / photoacoustic imaging, and has the advantage of gene transfection under the action of laser in vivo and in vitro. The preparation method and application of the multifunctional contrast agent.

Description

Technical field[0001]The present invention relates to the field of ultrasound, and more particularly to a method of preparing a multifunctional contrast agent generated and its application thereof.Background technique[0002]Retinoblastoma, RB) is the most common vulvar tumor in infants and young children, which occurs within 3 years old, has high degree of malignancy, and difficulties in early detection. At China, most of the children have grown significantly due to "white tangs", indicating that they have entered a period of serious harm and even life, may not be able to keep eyeballs, the treatment is still subject to the eyeballs, Completed by radiotherapy, chemotherapy and other means. Eyeball removal surgery has lost his eyes and never have three-dimensional visuals, and seriously affects the appearance of the child and physical and mental health; while the chemotherapy has a more obvious toxic side effect, radiotherapy can also lead to secondary tumors. The treatment effect is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K9/51A61K31/711A61P35/00
CPCA61K9/5146A61K31/711A61K49/0002A61K49/223A61K49/225
Inventor 周希瑗武明星邹宏密
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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