Preparation method of oyster polysaccharide compound

A technology of oyster polysaccharide and compound, which is applied in the field of preparation of oyster polysaccharide compound, and achieves the effects of simple and feasible preparation method, high resource utilization rate and reduced burden

Inactive Publication Date: 2018-05-11
金华市飞凌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the beverage is compounded with oyster polysaccharides and other substances, and it is rare to see oyster polysaccharides obtained by different extraction methods for compounding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The specific steps of the preparation method of oyster polysaccharide compound are:

[0016] Step 1: Grind and homogenize the fresh oyster meat, defatted with petroleum ether, dry and crush at low temperature to make defatted oyster powder;

[0017] Step 2: Mix defatted oyster powder and water at a material-to-liquid ratio of 1:20-30, leaching in boiling water for 8-12 minutes, filtering, and treating the residue 2-3 times in the same way. Combine the filtrate and adjust the pH of the total filtrate to 5.0-6.0, centrifuge at 8000-10000rpm for 22-28min, the supernatant obtained is passed through the ultrafiltration membrane, the filtrate after the membrane is spray-dried to obtain the water-extracted crude polysaccharide, and the precipitate is dried for use;

[0018] Step 3: Add 2-5% NaOH solution to the water-extracted precipitate at a material-to-liquid ratio of 1:18-22, then add 0.23-0.26% sodium borohydride and 0.014-0.017% Schiff base with the weight of the precipitate. ...

Embodiment 2

[0022] The specific steps of the preparation method of oyster polysaccharide compound are:

[0023] Step 1: Grind and homogenize the fresh oyster meat, defatted with petroleum ether, dry and crush at low temperature to make defatted oyster powder;

[0024] Step 2: Mix defatted oyster powder and water with a material-to-liquid ratio of 1:22, extract in boiling water for 12 minutes, filter, and process the filter residue 3 times in the same way. Combine the filtrate, adjust the pH of the total filtrate to 6.0, and centrifuge at 8000 rpm 28min, the obtained supernatant was passed through the ultrafiltration membrane, the filtrate after the membrane was spray-dried to obtain the water-extracted crude polysaccharide, and the precipitate was dried for use;

[0025] Step 3: Add 5% NaOH solution to the water-extracted precipitate at a material-to-liquid ratio of 1:18, then add 0.23% sodium borohydride and 0.017% Schiff base by weight of the precipitate, and extract at 55°C for 100 min, then ...

Embodiment 3

[0029] The specific steps of the preparation method of oyster polysaccharide compound are:

[0030] 1) Grind and homogenize fresh oyster meat, defatted with petroleum ether, dry and crush at low temperature to make defatted oyster powder;

[0031] 2) Mix defatted oyster powder and water with a material-to-liquid ratio of 1:25, extract with boiling water for 10 minutes, filter, and treat the filter residue twice in the same way. Combine the filtrate, adjust the pH of the total filtrate to 5.5, and centrifuge for 25 minutes at 9000rpm , The obtained supernatant is passed through an ultrafiltration membrane, and the filtrate after passing the membrane is spray-dried to obtain water-extracted crude polysaccharide, and the precipitate is dried for use;

[0032] 3) Add 3% NaOH solution to the water-extracted precipitate at a material-to-liquid ratio of 1:20, then add 0.25% sodium borohydride and 0.015% Schiff base by weight of the precipitate, and extract at 60°C for 90 minutes, and then ...

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PUM

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Abstract

The invention discloses an extraction method of oyster polysaccharides. The method comprises the following steps: degreasing fresh oyster meat; subjecting defatted oyster powder to extraction in boiling water, filtration and centrifuging, and drying supernatant to obtain water-extracted crude polysaccharides; adding alkali liquor to water-extracted precipitates, conducting extraction and centrifuging, and freeze-drying supernatant to obtain alkali-extracted crude polysaccharides; adding water to the crude polysaccharides for dissolving, sequentially adding trypsin and pepsin, conducting reaction and centrifuging, adding a Sevage reagent to supernatant, removing proteins, subjecting the upper layer of polysaccharide solution to centrifuging, taking precipitates, and conducting freeze-dryingto obtain the oyster polysaccharides; compounding and mixing the water-extracted oyster polysaccharides with the alkali-extracted oyster polysaccharides, and then preparing the obtained mixture intocapsules so as to obtain an oyster polysaccharide compound. The invention has the beneficial effects that the preparation method is simple and feasible, high in mechanized operation degree, high in resource utilization rate, low in energy consumption, and convenient for industrial scale production. The compound has protective effect on liver injury, and has the effects of improving heart functions, promoting blood circulation, and enhancing organism immunity.

Description

Technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a method for preparing an oyster polysaccharide compound. Background technique [0002] Oyster (oyster) is a bivalve mollusk belonging to the mollusk phylum Bivalvia pearl shell, Ostreidae (Ostreidae, true oyster) or Cockleshell (Aviculidae, pearl oyster), also known as oyster, sea oyster , Oyster yellow, oyster clam or clam. Oysters are the world’s largest cultured shellfish. About 100 species of oysters have been discovered worldwide. Oysters are also an important marine shellfish in my country. There are more than 20 kinds of oysters distributed along the coast of my country, including Oyster Oyster and Dalian. Bay oysters, Pacific oysters (Crassostrea gigas) and dense scale oysters. my country is a big oyster farming country, and oyster resources are extremely rich. Oysters are rich in physiologically active substances such as protein, polysaccharides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125C08B37/00
CPCA23L33/125A23V2002/00C08B37/003A23V2200/30A23V2200/326A23V2200/324A23V2250/51
Inventor 金韶京
Owner 金华市飞凌生物科技有限公司
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