Bacillus licheniformis ZL-1 and application thereof

Abstract

The invention discloses Bacillus licheniformis ZL-1 and the application thereof. The Bacillus licheniformis ZL-1 is obtained by isolating and screening from a high-temperature stack of feathers combined with edible fungus residues, the Bacillus licheniformis ZL-1 is preserved at Guangdong Provincial Microorganism Culture Collection Center, with a preservation number of GDMCC No. 60284, the preservation address is 5th floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou, China, and the preservation date is November 24, 2017. Experiments determine that the Bacillus licheniformisZL-1 can be cultured in a liquid culturing medium with pure feathers for three days, the keratinase activity of the Bacillus licheniformis ZL-1 reaches 52.14 U / ml, and the degradation rate of feathersreaches 90.15%. Further more, the Bacillus licheniformis ZL-1 has good tolerance to high temperature and salinity, can grow well at a temperature of 55 DEC G and is resistant to salinity of 14%, so that the Bacillus licheniformis ZL-1 is used for degrading the feathers, preparing compound amino acid solution, conducting high-temperature compost fermentation by using the feathers as raw materialsand preparing compound amino acid fertilizers and keratinase; the Bacillus licheniformis ZL-1 has good application prospects and market value.

Description

technical field [0001] The invention belongs to the technical field of microbes and their applications. More specifically, the invention relates to Bacillus licheniformis ZL-1 and its applications. Background technique [0002] Feathers are the waste left over from poultry processing. Worldwide, millions of tons of feather waste are produced every year, and nearly 700,000 tons of waste feathers are produced in my country every year. The problem of solid waste management has become poultry slaughtering. Business problems that need to be solved urgently. However, feather waste is a rich natural protein resource, its crude protein content is as high as 85%-90%, and it also contains vitamin B 12 and some unknown growth factors. Except for the significantly lower content of lysine and methionine in feather protein, the composition of other animal essential amino acids is slightly higher than that of fishmeal, which means that the production of compound amino acids by hydrolysis ...

AI Technical Summary

Problems solved by technology

[0004] The research on the screening and application of feather keratin-degrading bacteria in my country lags behind foreign countries. There are not many high-efficiency strains isolated and screened, and most of them are fungi and actinomycetes, and most of these reported keratin-degrading bacteria grow in normal temperature environment. keratin-degrading bacteria under high temperature conditions (above 45°C)

Since the optimum reaction temperature of keratinase is generally between 40°C and 60°C, the compost temperature in the composting fermentation using feathers as raw materials is generally above 50°C, which brings great difficulties to conventional biological treatment techniques.

Images