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Method for degrading high polymer procyanidine in receptaculum nelumbinis by utilizing solid acid

A technology of proanthocyanidin and solid acid is applied in the field of bioengineering, which can solve the problems of corrosion of instruments, difficult recovery, and difficult operation, and achieves the effects of mild reaction conditions, improved yield and simple operation.

Inactive Publication Date: 2018-05-08
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods can degrade high-polymer proanthocyanidins into oligomers, there are corresponding defects: the oxidant of the oxidative degradation method is selected from H 2 o 2 with KClO 3 , the catalyst is easy to decompose during use, and the degradation efficiency is affected; the acidic or alkaline solution used in the acidic degradation method and the alkaline degradation method will corrode the instrument; the acidic salt used in the sulfite method is not easy to recover after degradation; The equipment and environment requirements are high, and it needs to be carried out under high temperature and high pressure, so the operation is difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method utilizing solid acid to degrade high-polymer proanthocyanidins in lotus pods, the steps are as follows:

[0036] (1) Take 4g of degreased lotus pod powder, add 60ml of 60% ethanol solution, extract for 60min in a water bath at 50°C, centrifuge, recover ethanol, and obtain crude proanthocyanidin extract; extract and grade crude extract with ethyl acetate for 3 times , each classification needs to stand for 20 minutes, the water phase is the high-polymer proanthocyanidin suspension, which is diluted 10 times as the degradation raw material, and the average degree of polymerization is 5.95;

[0037] (2) Take 5g TiO 2 Powder (Degussa P25), add 50ml of 1mol / l sulfuric acid solution, impregnate for 1h, then filter with suction, vacuum dry the filter cake at 90°C for 24h, take it out and grind it, pass through a 100-mesh sieve, and then activate it in a muffle furnace at 400°C for 3h, Place to cool, get SO 4 2- / TiO 2 solid acid;

[0038] (3) Degradation: take 0....

Embodiment 2

[0043] A method utilizing solid acid to degrade high-polymer proanthocyanidins in lotus pods, the steps are as follows:

[0044](1) Take 4g of degreased lotus pod powder, add 60ml of 60% ethanol solution, extract for 60min in a water bath at 50°C, centrifuge and recover ethanol, and obtain crude proanthocyanidin extract; 3 times of extraction with ethyl acetate for graded crude extract , each classification needs to stand for 20 minutes, the water phase is the high-polymer proanthocyanidin suspension, which is diluted 10 times as the degradation raw material, and the average degree of polymerization is 5.95;

[0045] (2) Take 5g TiO 2 Powder (Degussa P25), add 50ml 2mol / l sulfuric acid solution, impregnate for 1h, then filter with suction, vacuum dry the filter cake at 90°C for 24h, take it out and grind it, pass through a 100-mesh sieve, and then activate it in a muffle furnace at 400°C for 3h, Place to cool, get SO 4 2- / TiO 2 solid acid;

[0046] (3) Degradation: take ...

Embodiment 3

[0051] A method utilizing solid acid to degrade high-polymer proanthocyanidins in lotus pods, the steps are as follows:

[0052] (1) Take 4g of degreased lotus pod powder, add 60ml of 60% ethanol solution, extract for 60min in a water bath at 50°C, centrifuge and recover ethanol, and obtain crude proanthocyanidin extract; 3 times of extraction with ethyl acetate for graded crude extract , each classification needs to stand for 20 minutes, the water phase is the high-polymer proanthocyanidin suspension, which is diluted 10 times as the degradation raw material, and the average degree of polymerization is 5.95;

[0053] (2) Take 5g TiO 2 Powder (Degussa P25), add 50ml 3mol / l sulfuric acid solution, impregnate for 1h, then filter with suction, vacuum dry the filter cake at 90°C for 24h, take it out and grind it, pass through a 100-mesh sieve, and then activate it in a muffle furnace at 400°C for 3h, Place to cool, get SO 4 2- / TiO 2 solid acid;

[0054] (3) Degradation: take...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a method for degrading high polymer procyanidine in receptaculum nelumbinis by utilizing solid acid. According to the method, low polymer procyanidine is obtained by performing degrading reaction and then separating and purifying by taking the high polymer procyanidine in the receptaculum nelumbinis as a raw material and taking SO4<2-> / TiO2 solid acid as a catalyst; The method for degrading the high polymer procyanidine in the receptaculum nelumbinis by utilizing the solid acid provided by the inventionimproves the yield of the low polymer procyanidine in the receptaculum nelumbinis; the adopted equipment are low in cost, the operation is simple, the reaction conditions are mild and the catalyst isrenewable; the application of the receptaculum nelumbinis in the fields, such as healthcare products, food, medicines and cosmetics, is expanded.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for degrading high-polymer proanthocyanidins in lotus pods by using solid acid. Background technique [0002] Proanthocyanidins are polymers formed by the dehydrogenation condensation of catechin monomers or epicatechin monomer molecules, including polymers with different molecular weights and isomers with the same degree of polymerization. Polymers with a degree of polymerization between 2 and 4 are oligomers, and proanthocyanidins with a degree of polymerization above 5 are high polymers. There is a negative correlation between the biological activity of proanthocyanidins and the degree of polymerization. Due to the large molecular weight of high polymers, they cannot freely pass through the cell layer when acting in plants, animals and humans, which will affect their absorption and utilization in the human body. The activity of the oligomer is very high, and t...

Claims

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Application Information

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IPC IPC(8): C07D311/62B01J27/053B01J37/02B01J37/08
CPCB01J27/053B01J37/0201B01J37/08C07D311/62
Inventor 张婕张惠陈卫航
Owner ZHENGZHOU UNIV
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