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Method for inducing adventitious buds from salvia prionitis inflorescences serving as explants

A technology of explants and red root grass, applied in the field of plant tissue culture, can solve the problems of unsatisfactory disinfection effect, difficulty in obtaining explants with high survival rate, etc., and achieve the effects of low pollution rate and high survival rate

Inactive Publication Date: 2018-05-08
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In view of the above-mentioned unsatisfactory disinfection effect, it is difficult to obtain explants with a higher survival rate, the present invention selects unopened young inflorescences extracted from vigorously growing red root grass scapes as explants, and provides a kind of explants with inflorescences as explants. The method of inducing adventitious buds of red root grass provides a reference for the rapid propagation of red root grass

Method used

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  • Method for inducing adventitious buds from salvia prionitis inflorescences serving as explants
  • Method for inducing adventitious buds from salvia prionitis inflorescences serving as explants
  • Method for inducing adventitious buds from salvia prionitis inflorescences serving as explants

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Embodiment 1

[0028] A method for inducing its adventitious buds with red root grass inflorescences as explants, comprising the steps of:

[0029] (1) Explant preparation: at noon when the weather is fine, select unopened young inflorescences from vigorously growing red root grass scapes, and use a scalpel to cut out about 1.0 cm in length as explants;

[0030] (2) Pretreatment of explants: Use a soft brush to dip the washing powder aqueous solution to gently scrub the surface of the explants, rinse with running water until there is no foam, and place in 50% carbendazim and 0.8 g / L vitamin C Soak in the prepared mixed solution for 25-30 min, rinse with tap water for 30 min, blot the surface moisture, and obtain the pretreated explant;

[0031] (3) Disinfection treatment and inoculation of explants: In the ultra-clean bench, soak the pretreated explants in 75% alcohol for 10s, and then transfer them into 0.1% mercury chloride + Tween-80 (Tween -80 volume ratio final concentration of 0.1%) i...

Embodiment 2

[0035] A method for inducing its adventitious buds with red root grass inflorescences as explants, comprising the steps of:

[0036] (1) Explant preparation: at noon when the weather is fine, select unopened young inflorescences from vigorously growing red root grass scapes, and use a scalpel to cut out about 1.0 cm in length as explants;

[0037] (2) Pretreatment of explants: Use a soft brush to dip the washing powder aqueous solution to gently scrub the surface of the explants, rinse with running water until there is no foam, and place in 50% carbendazim and 0.8 g / L vitamin C Soak in the prepared mixed solution for 25-30 min, rinse with tap water for 30 min, blot the surface moisture, and obtain the pretreated explant;

[0038] (3) Disinfection treatment and inoculation of explants: In the ultra-clean bench, soak the pretreated explants in 75% alcohol for 10s, and then transfer them into 0.1% mercury chloride + Tween-80 (Tween -80 (volume ratio, final concentration: 0.1%)) ...

Embodiment 3

[0043] A method for inducing its adventitious buds with red root grass inflorescences as explants, comprising the steps of:

[0044] (1) Explant preparation: at noon when the weather is fine, select unopened young inflorescences from vigorously growing red root grass scapes, and use a scalpel to cut out about 1.0 cm in length as explants;

[0045] (2) Pretreatment of explants: Use a soft brush to dip the washing powder aqueous solution to gently scrub the surface of the explants, rinse with running water until there is no foam, and place in 50% carbendazim and 0.8 g / L vitamin C Soak in the prepared mixed solution for 25-30 min, rinse with tap water for 30 min, blot the surface moisture, and obtain the pretreated explant;

[0046] (3) Disinfection treatment and inoculation of explants: In the ultra-clean bench, soak the pretreated explants in 75% alcohol for 10s, and then transfer them into 0.1% mercury chloride + Tween-80 (Tween -80 volume ratio final concentration of 0.1%) i...

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Abstract

The invention discloses a method for inducing adventitious buds from salvia prionitis inflorescences serving as explants. The method comprises the following steps: selecting unopened tender inflorescences grown from salvia prionitis scapes serving as the explants, dipping an aqueous detergent solution by using a soft-bristle brush to gently scrub dust on the surfaces, and performing disinfecting pretreatment in a mixed disinfectant liquid; then transferring to a superclean bench, firstly disinfecting the surfaces by using ethanol with the concentration of 75%, then immersing the explants in amixed solution of mercuric chloride and Tween for disinfecting, and finally rinsing for 3-4 times by using sterile water to obtain sterile tender inflorescences, and inoculating a primary culture medium with the sterile tender inflorescences for inducing the adventitious buds. By the method, the problems of a short collection time, a small quantity, difficult disinfection and high browning rate ofthe explants in salvia prionitis tissue culture are solved, enough explants can be obtained, the pollution rate of the salvia prionitis explants can be effectively reduced, and the survival rate of the explants is increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing adventitious buds by taking the inflorescences of red root grass as explants. Background technique [0002] Red Root Grass ( Salvia prionitis Hance) has antibacterial and detoxifying, heat-clearing and dehumidifying, anti-tuberculosis, anti-tumor effects, and is the main raw material of the national key protected traditional Chinese medicine "Compound Honggencao Tablet". The red root quinone extracted from red root grass is used as a key intermediate and chemically modified to form a diterpene quinone compound salvecin, which can inhibit cancer cells such as lung cancer, gastric cancer, ovarian cancer, liver cancer, colon cancer and cervical cancer Growth, blocking its dual role in repairing and regenerating pathways, and has the characteristics of low toxicity, anti-drug resistance and anti-metastasis, and is considered to be a new anti-tumor d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴群英唐辉龙晶王满莲彭智慧林军刘忠湫
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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