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Sperm motility fluid and preparation method thereof

A sperm motility and solution technology, applied in germ cells, vertebrate cells, animal cells, etc., can solve problems such as affecting the birth health of offspring, sperm DNA damage, etc., to ensure safety, improve vitality, and improve the effect of success rate

Pending Publication Date: 2018-05-04
瑞柏生物(中国)股份有限公司
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  • Abstract
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Problems solved by technology

However, the sperm agonists routinely used clinically in the prior art have a great damage effect on sperm DNA, directly affecting the birth health of offspring

Method used

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Embodiment Construction

[0027] The present invention will be further described below in combination with specific implementation methods.

[0028] The sperm motility liquid of the present invention is to make ATP sodium salt, heparin sodium dry powder / heparin sodium salt solution respectively according to the functionally feasible concentration (the preferred concentration is ATP sodium salt 5-20 μ g / ml, heparin sodium dry powder / heparin sodium salt solution) 10-50USP / ml) is prepared by dissolving in saline solution containing antibiotics and indicators. The antibiotic is gentamicin with a concentration of 5-11 μg / ml. The indicator is phenol red with a concentration of 9-10 μg / ml.

[0029] The saline solution is a compound culture solution using HEPES or MOPS as a buffer, based on sodium, potassium, magnesium, and calcium ions, and using glucose, sodium pyruvate, sodium lactate, and albumin as energy substances; it contains: Sodium 98.11-102.00mmol / L, potassium chloride 4.55-4.70mmol / L, magnesium s...

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Abstract

The invention relates to sperm motility fluid and a preparation method thereof, belonging to the technical field of human assisted reproduction. The sperm motility fluid is prepared by dissolving ATPsodium salt, dried heparin sodium powder / heparin sodium salt solution into a salt solution containing antibiotics and indicators. The ATP sodium salt and the heparin sodium are added into the sperm motility fluid disclosed by the invention, the ATP sodium salt can provide energy substances for sperm motility and contribute to sperm middle-piece mitochondria capacity, the heparin can activate a receptor-coupled signal transduction system by binding with a receptor on the sperm surface, the capacity function and calcium influx of the sperm mitochondria are gently and effectively improved, and the sperm motility is further improved. Therefore, the sperm motility can be effectively improved, the capacity of the sperm middle-piece mitochondria is wakened and activated, and the success rate of intra-uterine insemination (IUI) is improved. Moreover, a powerful guarantee is provided for the safety of an assisted reproductive technology.

Description

technical field [0001] The invention relates to a sperm motility liquid and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique [0002] In human assisted reproductive technology, for sperm with oligoasthenospermia, testicular and epididymal puncture, and frozen sperm due to lipid membrane damage and impaired mitochondrial function, the sperm motility can be greatly shortened (ICSI) and egg delivery time. The time of in vitro operation increases the fertilization rate and the number of available embryos, which directly or indirectly increases the pregnancy rate. However, the sperm agonists routinely used clinically in the prior art have a great damage effect on sperm DNA, directly affecting the birth health of offspring. [0003] Heparin sodium is a mucopolysaccharide sulfate anticoagulant drug, which is white or off-white powder, hygroscopic and easily soluble in water. Its functions include: 1. Treatment of ...

Claims

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Application Information

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IPC IPC(8): C12N5/076
Inventor 冯怀亮
Owner 瑞柏生物(中国)股份有限公司
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