Baby diaper rash cream and preparation method thereof
A technology for baby's buttock cream and baby, which is applied in the field of skin care, can solve the problems of the baby's tender skin that cannot be protected for a long time, treats the symptoms but not the root cause, and has poor effectiveness, so as to repair the baby's damaged skin, care for the recovery of damage, reduce the friction effect
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[0031] A kind of preparation method of baby's buttock cream, comprises the steps:
[0032] (1) Inoculate Streptococcus iniae into Streptococcus iniae fermentation medium at 3% inoculation amount, ferment at 35-40°C for 12-15 hours, then add dodecane with 70-80% mass of Streptococcus iniae fermentation medium Stir and mix sodium base sulfate, let stand for 10~15min, centrifuge, take supernatant A, add absolute ethanol at a mass ratio of 1:2, let stand at 3~4°C for 12~18h, centrifuge, take precipitate A with a mass fraction of Wash with 75% ethanol solution, dry to obtain fermented crude extract;
[0033] (2) Take the fermented crude extract and add sodium lauryl sulfate at a mass ratio of 100:1, let it stand for 10 minutes, centrifuge, take the supernatant B, add 10% hexadecane at a mass ratio of 30:1 Stir and mix the alkyltrimethylammonium bromide solution, centrifuge, take the precipitate B and add it to a sodium chloride solution with a concentration of 0.2 mol / L at a mass ...
Embodiment 1
[0036] Sea bream bone: purchased from Yunnan Haiwang Aquatic Products Co., Ltd., remove residual fish meat with a knife, wash with tap water, cut into small pieces, and store at -25°C.
[0037] Fermentation medium: in parts by mass, take 5 parts of glucose, 0.1 part of ferrous sulfate heptahydrate, 0.4 part of zinc sulfate heptahydrate, 0.002 part of sodium selenite pentahydrate, 0.007 part of magnesium sulfate heptahydrate, and 0.5 part of manganese sulfate , 0.6 parts of sodium chloride, 1 part of potassium dihydrogen phosphate, 0.1 part of dipotassium hydrogen phosphate, 1000 parts of water, adjust the pH to 7.0, and sterilize at 115°C for 30 min.
[0038] Bacteria: Lactobacillus plantarum, Bifidobacterium longum, cryopreserved on inclined planes.
[0039] Streptococcus iniae fermentation medium: in parts by mass, 1 part of tryptone, 0.5 part of sodium chloride, 0.25 part of disodium hydrogen phosphate, 0.2 part of glucose, 50 parts of bovine heart extract, 4 parts of gluco...
Embodiment 2
[0048] Sea bream bone: purchased from Yunnan Haiwang Aquatic Products Co., Ltd., remove residual fish meat with a knife, wash with tap water, cut into small pieces, and store at -25°C.
[0049] Fermentation medium: in parts by mass, take 6 parts of glucose, 0.2 part of ferrous sulfate heptahydrate, 0.5 part of zinc sulfate heptahydrate, 0.003 part of sodium selenite pentahydrate, 0.007 part of magnesium sulfate heptahydrate, 0.6 part of manganese sulfate , 0.7 parts of sodium chloride, 1 part of potassium dihydrogen phosphate, 0.3 parts of dipotassium hydrogen phosphate, 1000 parts of water, adjust the pH to 7.0, and sterilize at 115°C for 30 min.
[0050] Bacteria: Lactobacillus plantarum, Bifidobacterium longum, cryopreserved on inclined planes.
[0051] Streptococcus iniae fermentation medium: in parts by mass, 1 part of tryptone, 0.6 part of sodium chloride, 0.3 part of disodium hydrogen phosphate, 0.3 part of glucose, 52 parts of bovine heart extract, 4 parts of glucose, ...
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