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Camellia oleifera meal polypeptide, preparation method and application in cryopreservation of bovine semen

A technology of camellia oleifera meal polypeptide and cryopreservation, which is applied in the field of animal husbandry, can solve the problems that have not yet been reported on the in-depth development of hydrolyzed polypeptide of small fruit camellia oleifera meal, and achieve the effect of realizing waste reuse, easy implementation, and reducing oxidative damage

Active Publication Date: 2019-02-26
盐城新咏投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the in-depth development of hydrolyzed polypeptides of small fruit tea meal

Method used

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  • Camellia oleifera meal polypeptide, preparation method and application in cryopreservation of bovine semen
  • Camellia oleifera meal polypeptide, preparation method and application in cryopreservation of bovine semen
  • Camellia oleifera meal polypeptide, preparation method and application in cryopreservation of bovine semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of camellia oleifera meal polypeptide 1 by trypsin

[0047] Including the following steps:

[0048] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 150g / L;

[0049] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 3.0g / 100g based on the weight of meal powder), the enzymatic hydrolysis conditions are: pH value, 8.0; enzymatic hydrolysis temperature, 45°C; enzymatic hydrolysis time, 11 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;

[0050] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;

[0051] Step S4, dissolve the crude freeze-dried powder with ult...

Embodiment 2

[0052] Example 2: Preparation of camellia oleifera meal polypeptide 2 by trypsin

[0053] Including the following steps:

[0054] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 130g / L;

[0055] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 2.5g / 100g based on the weight of meal flour), the enzymatic hydrolysis conditions are: pH value, 7.8; enzymatic hydrolysis temperature, 43°C; enzymatic hydrolysis time, 12 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;

[0056] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;

[0057] Step S4, the crude freeze-dried powder is dissolved in ul...

Embodiment 3

[0058] Example 3: Preparation of camellia oleifera meal polypeptide 3 by trypsin

[0059] Including the following steps:

[0060] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 170g / L;

[0061] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 3.5g / 100g based on the weight of meal powder), the enzymatic hydrolysis conditions are: pH value, 8.2; enzymatic hydrolysis temperature, 47°C; enzymatic hydrolysis time, 10 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;

[0062] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;

[0063] Step S4, the crude freeze-dried powder is dissolved in ult...

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Abstract

The invention discloses an oil tea meal polypeptide, and a preparation method and an application thereof in cryopreservation of bovine semen. The oil tea meal hydrolytic polypeptide has a variety of activities, and the activities of the hydrolytic polypeptide are affected by proteolytic sites in the oil tea meal: the polypeptide produced by trypsin hydrolysis and having the molecular weight of [5000 u, 3000 u] has the effect of improving the antioxidant ability of the bovine cryopreserved semen, and can be used as a bovine semen cryopreservation protection agent for reducing oxidative damage;and the polypeptide produced by alkaline protease hydrolysis and having the molecular weight of [5000 u, 3000 u] can promote empathema and pregnancy of anestrus cows. Moreover, the preparation methodof the hydrolytic polypeptide is simple, only simple crushing, enzymatic hydrolysis, ultrafiltration and freeze drying are used, and all the steps can be realized in a factory with mass production andare easy to implement. Waste reuse of camellia meiocarpa hu. meal is realized, and the economic added value of the camellia meiocarpa hu. meal can be increased.

Description

technical field [0001] The invention belongs to the field of animal husbandry, and relates to a camellia oleifera meal polypeptide, a preparation method and an application in the frozen preservation of bovine semen. Background technique [0002] Camellia meiocarpa Hu. is the main species of Camellia oleifera (Camellia oleifer aAbel.) in terms of cultivation area and annual output in my country. It is mainly distributed in Fujian, Jiangxi and Guangxi south of Huaihe River and Yangtze River in my country. , Hunan, Guizhou and other places. Small fruit camellia is also known as Jiangxizi, small tea, chicken heart, small leaf camellia, sheep excrement and so on. The fruit is small, the leaves are small, the buds are small, and the buds and bracts are not hairy. [0003] Camellia oleifera meal is a by-product obtained after extracting oil from Camellia oleifera. Although tea farms have detoxified these camellia leaves and used them as animal feed, due to the immature technology...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K2/00C12P21/06C07K1/34A01N1/02
CPCA01N1/0226C07K2/00C12P21/06
Inventor 盛蓓蓓李沛王崇智
Owner 盐城新咏投资发展有限公司
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