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Application of resuscitation solution obtaining rich hydrogen sulfide to preparation of medicine for treating hemorrhagic shock

A technology of resuscitation fluid and hydrogen sulfide, which is applied in the field of medicine, can solve problems such as ischemia-reperfusion injury, organ failure, and tissue perfusion insufficiency, and achieve the effects of improving microcirculation, protecting cardiomyocytes, and inhibiting cardiomyocyte damage

Inactive Publication Date: 2018-04-24
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Hemorrhagic shock can lead to tissue hypoperfusion, cell metabolism disorder, ischemia-reperfusion injury, organ failure and death. In the corresponding treatment methods, there is no relevant information about the use of hydrogen sulfide-rich resuscitation fluid to prevent and treat hemorrhagic shock. There are no reports on the use of resuscitation fluid rich in hydrogen sulfide to prevent myocardial cell injury caused by hemorrhagic shock

Method used

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  • Application of resuscitation solution obtaining rich hydrogen sulfide to preparation of medicine for treating hemorrhagic shock
  • Application of resuscitation solution obtaining rich hydrogen sulfide to preparation of medicine for treating hemorrhagic shock
  • Application of resuscitation solution obtaining rich hydrogen sulfide to preparation of medicine for treating hemorrhagic shock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of resuscitation fluid rich in hydrogen sulfide

[0032] The first step, degassing treatment: put the 500mL physiological saline injection soft packaging bag in a low-pressure chamber, decompress at 0.4 absolute atmospheric pressure at room temperature for 2 hours, take it out and place it at room temperature and pressure for 4 hours, and remove the precipitated gas Withdraw with a syringe.

[0033] The second step, low-temperature pretreatment: place the above-mentioned degassed saline injection flexible packaging bag in an environment of 2° C. for 2 hours to fully cool under normal pressure.

[0034] Step 3, configure hydrogen sulfide solution: extract 50mL of physiological saline from the soft packaging bag after the above-mentioned low-temperature pretreatment, inject it into a closed container with solid sodium hydrogen sulfide 8.4mg (molecular weight 56), filter after fully dissolving, and The filtered hydrogen sulfide solution is injected ...

Embodiment 2

[0037] Embodiment 2: animal experiments

[0038] 1. Experimental animals: adult healthy Beagle dogs with a body weight of 12-15kg were purchased from the Experimental Animal Center of the Second Military Medical University.

[0039] Take 13 adult healthy Beagle dogs and divide them into 3 groups randomly:

[0040] Normal control group (Con), n=3;

[0041] Simple normal saline resuscitation group (NS), n=5;

[0042] Hydrogen sulfide-rich resuscitation fluid resuscitation group (H 2 S), n=5.

[0043] Except for the normal control group, hemorrhagic shock models were established in the other groups, and different resuscitation groups were given different resuscitation fluids for resuscitation after the hemorrhagic shock models were successfully established.

[0044] 2. Preparation of hemorrhagic shock model:

[0045] After the experimental dog was anesthetized with pentobarbital sodium, it was fixed on the operating table, and the left femoral artery, left jugular vein, and ...

Embodiment 3

[0093] Example 3: Preparation of resuscitation fluid rich in hydrogen sulfide

[0094] The first step, degassing treatment: place a 500mL physiological saline injection soft packaging bag in a low pressure chamber, decompress at room temperature at 0.8 absolute atmospheric pressure for 24 hours, take it out and place it at room temperature and pressure for 12 hours, and remove the precipitated gas Withdraw with a syringe.

[0095] The second step, low-temperature pretreatment: place the above-mentioned degassed saline injection flexible packaging bag in an environment of 0°C for 2 hours to fully cool under normal pressure.

[0096] The third step, configure the hydrogen sulfide solution: extract 50mL of physiological saline from the soft packaging bag after the above-mentioned low-temperature pretreatment, inject it into a closed container with 70 mg of solid sodium hydrogen sulfide (molecular weight 56), filter it after fully dissolving, and filter The final hydrogen sulfide...

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Abstract

The invention discloses application of a resuscitation solution with rich hydrogen sulfide to preparation of a medicine for treating hemorrhagic shock. According to the application, the medicine for treating the hemorrhagic shock is the resuscitation solution with the rich hydrogen sulfide; the concentration of the resuscitation solution with the rich hydrogen sulfide is 250 to 650mu mol / L. A preparation method of the resuscitation solution with the rich hydrogen sulfide comprises the following steps: taking the resuscitation solution which is subjected to degassing treatment and low-temperature pre-treatment in sequence, and dissolving solid sodium hydrosulfide or solid hydrogen sulfide; after filtering, carrying out deep-low-temperature cryopreservation; in a utilization process, dissolving a hydrogen sulfide solution, obtained by sufficient dissolution, into the resuscitation solution, so as to obtain the resuscitation solution with the rich hydrogen sulfide. According to the application of the resuscitation solution with the rich hydrogen sulfide to the preparation of the medicine for treating the hemorrhagic shock, disclosed by the invention, an animal experiment proves that the resuscitation solution with the rich hydrogen sulfide can be used for stabilizing haemodynamics in a resuscitation process after the hemorrhagic shock, improving a cardiac function, improving microcirculation and inhibiting cardiac muscle cell injuries, so that the rescue and curative rate and the survival rate of the hemorrhagic shock are improved.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to the application of a resuscitation fluid rich in hydrogen sulfide in the preparation of medicines for treating hemorrhagic shock. Background technique [0002] Hemorrhagic shock refers to the pathophysiological process of acute massive loss of blood or plasma caused by various reasons, resulting in reduction of effective circulating blood volume and cardiac output, tissue hypoperfusion, cell metabolism disorder and functional impairment. In the early stage of shock, that is, in the compensatory stage, the body's small blood vessels continue to contract and spasm, a large number of true capillary networks are closed, microcirculation blood perfusion is reduced, and tissue ischemia and hypoxia. At the same time, the sympathetic nervous system is excited, and the sympathetic adrenal medullary system causes the increase of catecholamines in the body fluid and the secretion of other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K9/08A61P7/08
CPCA61K9/08A61K33/04A61K47/02
Inventor 肖健乐士冠王长楠奚望王擎王婧李伟王志农乌立晖徐激斌杨潜
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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