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AaTCP15 gene promoter preferentially expressed in glandular trichomes of Artemisia annua L. , and application thereof

A promoter and gene technology, applied in the field of molecular biology, can solve the problems of normal plant growth burden, excessive consumption of cellular material and energy, regulation of target genes in time and space, etc.

Active Publication Date: 2018-04-20
SUZHOU TANGJI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In the current genetic engineering research of Artemisia annua, constitutive promoters are often used, such as the cauliflower mosaic virus 35S promoter (CaMV35S), which can drive the expression of foreign genes in all tissues and organs, and will cause excessive consumption. The substance and energy in the cell, and the expression of the target gene cannot be regulated in time and space, which is likely to cause a certain burden and harm to the normal growth of plants

Method used

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  • AaTCP15 gene promoter preferentially expressed in glandular trichomes of Artemisia annua L. , and application thereof
  • AaTCP15 gene promoter preferentially expressed in glandular trichomes of Artemisia annua L. , and application thereof
  • AaTCP15 gene promoter preferentially expressed in glandular trichomes of Artemisia annua L. , and application thereof

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Embodiment Construction

[0023] The experimental method that does not indicate specific conditions in the following examples is usually according to conventional conditions, such as molecular cloning such as Sambrook: the laboratory manual sees the conditions described in the 1989 edition of New York:Cold Spring Harbor Laboratory Press, or according to the manufacturer suggested conditions.

[0024] The Agrobacterium tumefaciens EHA105 involved in this embodiment has been described in "Huang Yali, Jiang Xiliang, Tian Yunlong, Guo Ping, Zhu Changxiong; Research on the genetic transformation of Trichoderma harzianum mediated by Agrobacterium tumefaciens, Chinese Journal of Biotechnology, 2008, 28( 3): 38-43" published in the literature. For Agrobacterium tumefaciens EHA105, the plasmid pCAMBIA-1391z can be obtained through publicly available commercial channels, for example, it can be purchased from CAMBIA Company in Australia, and the strain number is Gambar1.

[0025] This embodiment relates to the a...

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Abstract

The invention provides a gene promoter, belonging to the field of plant biotechnology. The gene promoter has a nucleotide sequence as shown in SEQ ID No. 1. The AaTCP15 gene promoter can regulate theexpression of a target gene in all the tissue of a plant, especially preferential expression in secretory glandular trichomes and non-secretory glandular trichomes. Meanwhile, the invention also relates to application of the AaTCP15 gene promoter to genetic engineering breeding which employs the glandular trichome tissue of plants for expression and production metabolites. Since genetic manipulation of the promoter preferentially expressed in glandular trichomes on the glandular trichome system of plants has no harm to the growth and development of the plants, the AaTCP15 gene promoter provided by the invention is of critical significance to genetic engineering breeding which employs the glandular trichome tissue of plants for expression and production metabolites.

Description

technical field [0001] The invention relates to a technique in the field of molecular biology, in particular to a promoter of the AaTCP15 gene predominantly expressed in glandular hairs of Artemisia annua and its application. Background technique [0002] Artemisia annua L. is an annual herb of the genus Artemisia in the family Asteraceae, also known as Artemisia annua, with a plant height of about 30-150cm. In 1972, Tu Youyou and her team isolated Artemisinin, an effective antimalarial monomer, from Artemisia annua. Artemisinin is a sesquiterpene lactone compound containing a peroxo bridge structure, which is the main active ingredient of antimalarial drug combination therapies (Artemisinin-based combination therapies, ACTs) recommended by the World Health Organization (WHO). In recent years, artemisinin and its derivatives have been reported to have the effects of treating lupus erythematosus, Alzheimer's disease, anti-cancer and lowering blood lipids. The current produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82C12Q1/6806A01H5/00
CPCC12N9/00C12N15/8223C12Q1/6806
Inventor 唐克轩马亚男徐东北付雪晴沈乾石璞黎凌陈明慧谢利辉孙小芬
Owner SUZHOU TANGJI BIOTECH CO LTD
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