Oleophylic water-coating immobilized microorganism carrier and preparation method thereof

A technology for immobilizing microorganisms and immobilizing carriers, applied in microorganism-based methods, biochemical equipment and methods, immobilizing on or in inorganic carriers, etc. Secondary pollution and other problems, to achieve the effect of no toxic effect, good activity stability, and strong adsorption

Active Publication Date: 2018-04-20
SHANDONG JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many carrier materials have been reported for oil spill remediation, however, finding materials that are both oil-absorbent and buoyant is not an easy task
Activated carbon is the most widely used immobilized carrier, such as rice straw-based, corncob-based, and peanut shell-based activated carbon carriers prepared by publication numbers CN103923905A, CN103923904A, and CN103923903A. The adsorption capacity for oil spill is low; the public number CN104004745A uses calcium alginate-mussel shells to immobilize marine bacteria, which has the advantages of low cost, non-toxic and harmless, and high vitality, but the carrier does not have buoyancy; polyvinyl alcohol is used as a package The embedded carrier has the advantages of high mechanical strength, strong anti-decomposition ability, and low price. For example, the publication number CN103194437A uses polyvinyl alcohol-boric acid secondary cross-linking to complete the immobilization of bacteria for sewage treatment, which reduces the damage to bacteria during the immobilization process. Effectively reduce the problems of adhesion and water-soluble swelling, but polyvinyl alcohol does not have buoyancy, so it cannot be used to treat oil film on the sea surface
Polyurethane foam is a commonly used carrier material, such as the nitrifying bacteria immobilized bioactive filler of the polyurethane carrier invented by the publication number CN103951039A, which has buoyancy, but is not easy to degrade and easily causes secondary pollution

Method used

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  • Oleophylic water-coating immobilized microorganism carrier and preparation method thereof
  • Oleophylic water-coating immobilized microorganism carrier and preparation method thereof
  • Oleophylic water-coating immobilized microorganism carrier and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0056] Such as figure 1 Shown, the preparation method of lipophilic water-in-water immobilized microbial carrier in the present embodiment, it comprises the following steps:

[0057] (1) Preparation of intermediate amphiphilic graphene

[0058] a.Carboxylation reaction

[0059] Weigh 2.0g of graphene and put it into a 100ml stoppered Erlenmeyer flask, add 5.0ml of concentrated nitric acid and soak for 1h for acidification treatment to initiate carboxyl groups;

[0060] b. Acylation reaction

[0061] Add 8.0 g of thionyl chloride to the acidified material for acylation reaction, place it on a constant temperature water bath shaker at 40°C at a rate of 120 rpm for 1 hour, and wash the product with deionized water until neutral;

[0062] c. Hydroxylation reaction

[0063] Add 25ml of ethylene glycol into the conical flask as a hydroxyl source, place it on a constant temperature water bath shaker at 40°C and react at a rate of 120rpm for 1h to obtain graphene containing hydrox...

Embodiment 2

[0087] refer to figure 1 , the preparation method of lipophilic water-in-water immobilized microbial carrier in the present embodiment, it comprises the following steps:

[0088] (1) Preparation of intermediate amphiphilic graphene

[0089] a.Carboxylation reaction

[0090] Weigh 2.0g of graphene and put it into a 100ml stoppered Erlenmeyer flask, add 8.0ml of concentrated nitric acid to soak for 1h for acidification treatment to initiate carboxyl groups;

[0091] b. Acylation reaction

[0092] Add 2.0 g of thionyl chloride to the acidified material for acylation reaction, place it on a constant temperature water bath shaker at 40°C at a rate of 120 rpm for 1 hour, and wash the product with deionized water until neutral;

[0093] c. Hydroxylation reaction

[0094] Add 25ml of ethylene glycol into the conical flask as a hydroxyl source, place it on a constant temperature water bath shaker at 40°C and react at a rate of 120rpm for 1h to obtain graphene containing hydroxyl gr...

Embodiment 3

[0117] Refer to attached figure 1 , the preparation method of lipophilic water-in-water immobilized microbial carrier in the present embodiment, it comprises the following steps:

[0118] (1) Preparation of intermediate amphiphilic graphene

[0119] a.Carboxylation reaction

[0120] Weigh 2.0g of graphene into a 100ml Erlenmeyer flask with a stopper, add, soak in 10.0ml of concentrated nitric acid for 1h to acidify and initiate carboxyl groups;

[0121] b. Acylation reaction

[0122] Add 10.0 g of thionyl chloride to the acidified material for acylation reaction, place it on a constant temperature water bath shaker at 40°C at a rate of 120 rpm for 1 hour, and wash the product with deionized water until neutral;

[0123] c. Hydroxylation reaction

[0124] Add 30ml of ethylene glycol into the conical flask as a hydroxyl source, place it on a constant temperature water bath shaker at 40°C and react at a rate of 120rpm for 1h to obtain graphene containing hydroxyl groups, and ...

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Abstract

The invention belongs to the field of preparation of porous materials, and particularly relates to an oleophylic water-coating immobilized microorganism carrier for degrading spilled oil and a preparation method thereof. The oleophylic water-coating immobilized microorganism carrier is characterized in that graphene is used as a matrix material, the molecules of the graphene matrix material are designed, the surface carries the hydrophilic groups of hydroxyl and carboxyl groups and the double-bond hydrophobic polymerizing groups, and then an amphiphilic structure is formed, so that the amphiphilic characteristics of oleophylic performance and hydrophilicity is realized, the immobilized microorganism carrier can float onto the water surface, and favorably absorb the spilled oil floating onthe water surface, and the necessary sunlight, air and water are provided for the growth of microorganisms; the graphene with amphiphilic structure is crosslinked with a lipophilic organic polymer, sothat the carrier has the weak-polarity affinity, and can mutually attract with oil droplets to directionally move, the oil droplets are quickly adsorbed onto the surface of the carrier, the adsorbingability is strong, and the easiness in falling is avoided. The prepared immobilized microorganism carrier has the advantage that when the spilled oil is degraded, the degrading rate is greatly improved, and the function of restoring the environment at the oil-spilling sea is good.

Description

technical field [0001] The invention belongs to the field of preparation of porous materials, in particular to a lipophilic water-in-water immobilized microbial carrier for degrading oil spills and a preparation method thereof. Background technique [0002] Offshore oil exploration and ship oil transportation, oil spill accidents are becoming more and more frequent. Serious oil spill pollution is a common disaster worldwide. Domestic oil spill accidents that have a greater impact include Dalian Bay, Penglai ConocoPhillips Oilfield, and Qingdao Huangdao Oil Spill, which not only caused huge damage economic losses, and bring ecological disasters that are difficult to repair to the marine environment. It is the responsibility of all marine countries, governments and scientific research departments to study efficient methods of removing oil pollution and restore the polluted ecological environment. The mainstream cleaning methods currently used are physical methods and chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/04C12N11/02C02F3/34C12R1/01
CPCC02F3/34C02F3/348C12N11/02C12N11/04C12N11/14
Inventor 张少君王明雨孟雄飞常青
Owner SHANDONG JIAOTONG UNIV
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