TCM (traditional Chinese medicine) micro-ecology for treating haemonchosis contortus in Jianchang black goat and preparation method
A technology of Haemonchus contortus and traditional Chinese medicine microecology, applied in the field of livestock breeding
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[0021] The preparation method of the bacillus subtilis bacterium liquid is as follows:
[0022] Use LB medium to activate Bacillus subtilis strains, and then expand and cultivate Bacillus subtilis until the bacterial concentration reaches 0.5×10 8 -1×10 8 CFU / mL is obtained.
[0023] The preparation method of described Lactobacillus calf bacterial liquid is as follows:
[0024] Using MRS medium to activate Lactobacillus calf strains, and then expand and cultivate Lactobacillus calf until the concentration of the bacteria solution reaches 0.5×10 8 -1×10 8 CFU / mL is obtained.
[0025] The present invention also provides the preparation method of above-mentioned Chinese medicine microecology:
[0026] Mix the above-mentioned Shijunzi, Changshan, Citrus aurantium, Bupleurum, Betel nut, Amomum, Tangerine Peel, Shouwu, and Atractylodes in parts by weight, grind them, add 300-400g of water per 100g of the mixture, and boil in a pot for 3 hours , filter to obtain the filtrate, t...
Embodiment 1
[0030] Embodiment 1: the prescription of Chinese medicine microecology 1
[0031] 38 parts by weight of Shijunzi, 31 parts by weight of Changshan, 22 parts by weight of Citrus aurantii, 14 parts by weight of Bupleurum, 12 parts by weight of betel nut, 6 parts by weight of amomum, 8 parts by weight of tangerine peel, 17 parts by weight of Shouwu, 12 parts by weight of atractylodes; The volume ratio of the Bacillus subtilis bacteria solution to the Chinese medicine mixture is 2.5:100; the volume ratio of the Lactobacillus calf bacteria solution to the Chinese medicine mixture is 3:100.
Embodiment 2
[0032] Embodiment 2: the prescription of Chinese medicine microecology 2
[0033] 35 parts by weight of Shijunzi, 34 parts by weight of Changshan, 29 parts by weight of Citrus aurantii, 11 parts by weight of Bupleurum, 14 parts by weight of betel nut, 8 parts by weight of amomum, 6 parts by weight of tangerine peel, 19 parts by weight of Shouwu, 14 parts by weight of atractylodes; The volume ratio of the Bacillus subtilis liquid to the Chinese medicine mixture is 3:100; the volume ratio of the Lactobacillus calf bacteria liquid to the Chinese medicine mixture is 2.5:100.
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