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Antibacterial collagen preparation method based on epoxidation modification of safrole

A technology of safrole and epoxidation, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of continuous dissolution antibacterial mode, lack of long-term effect, and affect the biocompatibility of natural collagen, etc., to achieve antibacterial The effect of long-lasting function and mild reaction conditions

Active Publication Date: 2018-04-13
YIBIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the continuous dissolution antibacterial mode must lack long-term effect
Secondly, while many traditional antibacterial agents effectively kill harmful microorganisms such as bacteria, they also show physiological toxicity to mammals, and their addition will affect the inherent biocompatibility of natural collagen

Method used

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  • Antibacterial collagen preparation method based on epoxidation modification of safrole
  • Antibacterial collagen preparation method based on epoxidation modification of safrole

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Safrole epoxidation modification:

[0022] Dissolve 12 parts of m-chloroperoxybenzoic acid in 100 parts of dichloromethane, add 8 parts of safrole, and stir at 20°C for 24 hours; thereafter, the product is washed 3 times with 2% aqueous sodium hydroxide solution, and then used to remove Ionized water was washed 3 times; finally, the oil layer was dried with anhydrous magnesium sulfate, filtered and distilled under reduced pressure to obtain epoxidized safrole;

[0023] (2) Epoxidized safrole grafted modified collagen:

[0024] Dissolve 10 parts of collagen in 0.2mol / L acetic acid aqueous solution, then coat the above mixture to form a film, and dry it at 20°C to constant weight; then soak the above collagen film in pH value 7, epoxidized safrole concentration 3% aqueous solution; after 12 hours, the collagen film was taken out and washed three times with deionized water to obtain epoxidized safrole grafted modified collagen.

[0025] The results of infrared spectr...

Embodiment 2

[0027] (1) Safrole epoxidation modification:

[0028] Dissolve 15 parts of iodosobenzene in 150 parts of chloroform, add 10 parts of safrole, and stir for 20 hours at 25°C; after this, the product is washed 4 times with 5% potassium hydroxide aqueous solution, and then washed with deionized water for 5 time; at last, the oil layer is dried with anhydrous magnesium sulfate, and after filtration, vacuum distillation obtains epoxidized safrole;

[0029] (2) Epoxidized safrole grafted modified collagen:

[0030] Dissolve 15 parts of collagen in 0.4 mol / L acetic acid aqueous solution, then coat the above mixture to form a film, and dry it at 22°C to constant weight; then soak the above collagen film in pH 8, epoxidized safrole concentration 7% aqueous solution; after 24 hours, the collagen film was taken out and washed 4 times with deionized water to obtain epoxidized safrole grafted modified collagen.

[0031] The results of infrared spectroscopy and circular dichroism experimen...

Embodiment 3

[0033] (1) Safrole epoxidation modification:

[0034] Dissolve 20 parts of tert-butanol peroxide in 200 parts of carbon tetrachloride, add 12 parts of safrole, and stir at 30° C. for 12 hours; after this, the product is washed 5 times with 7% aqueous sodium bicarbonate solution, and then used to remove Washing with deionized water for 5 times; finally, the oil layer was dried with anhydrous magnesium sulfate, filtered and distilled under reduced pressure to obtain epoxidized safrole;

[0035] (2) Epoxidized safrole grafted modified collagen:

[0036] Dissolve 20 parts of collagen in 0.5 mol / L acetic acid aqueous solution, then coat the above mixture to form a film, and dry it at 25°C to constant weight; then soak the above collagen film in pH 9, epoxidized safrole concentration 10% aqueous solution; after 48 hours, the collagen film was taken out and washed 5 times with deionized water to obtain epoxidized safrole grafted modified collagen.

[0037] The results of infrared s...

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Abstract

The invention discloses an antibacterial collagen preparation method based on epoxidation modification of safrole. According to the method, safrole is modified with a weak epoxidation reagent, and themodified safrole is covalently introduced into collagen by using the covalent reaction between the epoxy group and the amino group on the side chain of the collagen macromolecule, such that the collagen substrate has good antibacterial activity. According to the present invention, the collagen material obtained by using the modification method has the long-lasting antibacterial function, whereinthe three-stranded spiral structure of the collagen substrate cannot be destroyed, and the inherent biocompatibility can be completely retained, such that the application prospect in the collagen-based biomedical material field is broad.

Description

technical field [0001] The invention relates to a method for preparing antibacterial collagen based on safrole epoxidation modification, which belongs to the field of biomass materials. Background technique [0002] Collagen is the most important structural protein in the connective tissue of higher vertebrates. It exists widely in animal tissues in the form of skin, bones, tendons, ligaments, nerves, blood vessels, and teeth, and plays a role in supporting organs and protecting biological organisms. Collagen is the most abundant protein in mammals, accounting for about 25-33% of the total protein in animals. The special triple-helix conformation of collagen endows it with many excellent biological properties, such as degradability, hemostasis, and tissue compatibility, so that it can be used in tissue engineering such as skin substitutes, artificial cartilage, and artificial blood vessels, as well as in various fields. It has been widely used in a variety of medicine and h...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/113C07K1/02
CPCC07K14/78
Inventor 徐洲范浩军陈意常金明官小玉王忠辉曾琦
Owner YIBIN UNIV
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