A kit for measuring glycated serum albumin by enzymatic chemiluminescence
The technology of serum albumin and luminescence method is applied in the field of kits for measuring the concentration ratio of glycated serum albumin and albumin by enzyme chemiluminescence method, which can solve the problems of complex construction of protease co-expression body, unfavorable promotion and high cost, and achieves the improvement of Specificity, high precision of detection results, and improved accuracy
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Embodiment 1
[0065] Embodiment 1 Enzyme chemiluminescence assay kit for glycosylated serum albumin
[0066] Example 1 The kit for measuring glycated serum albumin by enzymatic chemiluminescence method includes reagent R1, reagent R2 and reagent R3, wherein the reagent R1 comprises the following components: urea phosphate 10 mmol / L, triethanolamine 1 mmol / L, pancreatic Protease 1U / mL, anti-interference agent 0.02%, proclin 300 0.05%, pH 7.5 MES buffer 10mmol / L, the anti-interference agent is composed of chloroacetyl chloride and N-chloroacetamide in a mass ratio of 1:0.5 .
[0067] The reagent R2 comprises the following components: 8 U / mL of fructosamine oxidase, 10 g / L of trehalose and 10 mmol / L of pH7.5 phosphate buffer.
[0068] The reagent R3 comprises the following components: amino acid oxidase 20U / mL, trehalose 10g / L and pH7.5 phosphate buffer 10mmol / L.
[0069] The reagent R4 is a commercially available luminescence substrate 1,2-dioxetaneboronic acid.
Embodiment 2
[0070] Embodiment 2 Enzyme chemiluminescence assay kit for glycosylated serum albumin
[0071] Example 2 The kit for measuring glycated serum albumin by enzymatic chemiluminescence method includes reagent R1, reagent R2 and reagent R3, wherein the reagent R1 comprises the following components: 30 mmol / L of urea phosphate, 3 mmol / L of triethanolamine, pancreatic Protease 1U / mL, anti-interference agent 0.01%, proclin 300 0.06%, pH 8.0 MES buffer 30mmol / L, the anti-interference agent is composed of chloroacetyl chloride and N-chloroacetamide in a mass ratio of 1:0.6 .
[0072] The reagent R2 comprises the following components: fructosamine oxidase 10 U / mL, trehalose 20 g / L and pH 8.0 phosphate buffer 20 mmol / L.
[0073] The reagent R3 comprises the following components: amino acid oxidase 30 U / mL, trehalose 20 g / L and pH 8.0 phosphate buffer 20 mmol / L.
[0074] The reagent R4 is a commercially available luminescence substrate 1,2-dioxetaneboronic acid.
Embodiment 3
[0075] Example 3 Kit for Determination of Glycated Serum Albumin by Enzyme Chemiluminescence
[0076] Example 3 The kit for measuring glycated serum albumin by enzymatic chemiluminescence method includes reagent R1, reagent R2 and reagent R3, wherein the reagent R1 comprises the following components: 40 mmol / L of urea phosphate, 4 mmol / L of triethanolamine, pancreatic Protease 1U / mL, anti-interference agent 0.03%, proclin 300 0.04%, pH 8.5 MES buffer 40mmol / L, the anti-interference agent is composed of chloroacetyl chloride and N-chloroacetamide in a mass ratio of 1:0.6 .
[0077] The reagent R2 comprises the following components: fructosamine oxidase 20U / mL, trehalose 20g / L and pH8.5 phosphate buffer 40mmol / L.
[0078] The reagent R3 comprises the following components: amino acid oxidase 50 U / mL, trehalose 20 g / L and pH 8.5 phosphate buffer 20 mmol / L.
[0079] The reagent R4 is a commercially available luminescence substrate 1,2-dioxetaneboronic acid.
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