Detection method of trace amount of target based on monomolecular fluorescent sensing
A detection method and fluorescence sensing technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as affecting repeatability and reliability, reducing solution fluorescence background, reducing sensitivity and specificity, etc. Highly selective and sensitive, improved sensitivity and specificity, easy-to-prepare effects
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[0052] Example 1: Detection of Acetamiprid and PCB-77 by Single Molecule Fluorescence Sensing
[0053] (1) Surface modification of microscope slides
[0054] Disposable slides were sonicated in 1 M KOH solution for 20 min and rinsed with water. Boil in 14% hydrogen peroxide / ammonia for more than 20 minutes, rinse with water, then sonicate in methanol for 15 minutes and rinse with acetone. Put a certain amount of deionized water at the bottom of the empty pipette tip box to maintain a humid environment. Place the above blow-dried slides on the rack of the pipette tip box.
[0055] A 0.1 M sodium bicarbonate solution was prepared as PEG buffer. Prepare a solution containing 250mg / ml mPEG and 25mg / ml biotin-PEG with this buffer, immediately drop 120μL on each slide, and cover it with another slide without air bubbles. Reacted for 3 hours at room temperature, rinsed with water and blown dry with nitrogen, with the modified surface facing up on the rack of the pipette tip box. ...
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