Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial strain of Bacillus spp. for high-efficient wide-spectrum decolorization and degradation of reactive dyes and application thereof

A reactive dye, high-efficiency and broad-spectrum technology, applied in the field of microorganisms and wastewater treatment, can solve the problems of single function of degrading strains, incomplete degradation, complex components, etc., and achieve the effect of good theoretical research value and production and application prospects.

Active Publication Date: 2018-03-27
HUANGGANG NORMAL UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of high content of organic matter in printing and dyeing wastewater caused by the widespread use of reactive dyes, complex components, single function of degradation strains, and incomplete degradation, etc., and provide a Bacillus strain for efficient broad-spectrum decolorization and degradation of reactive dyes and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain of Bacillus spp. for high-efficient wide-spectrum decolorization and degradation of reactive dyes and application thereof
  • Bacterial strain of Bacillus spp. for high-efficient wide-spectrum decolorization and degradation of reactive dyes and application thereof
  • Bacterial strain of Bacillus spp. for high-efficient wide-spectrum decolorization and degradation of reactive dyes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Isolation, screening and identification of embodiment 1HM01 bacterial strain

[0021] (1) Preparation of culture medium

[0022] Inorganic salt medium: KH 2 PO 4 0.5g / L, K 2 HPO 4 1.5g / L, NaCl 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, 1.5mL of trace element solution was added to 1L medium (Zheng Yongliang, Zhong Yulin, Xu Hong, et al. Screening and characteristics of dye decolorization strains[J]. Journal of Huazhong Normal University: Natural Science Edition, 2008 , 03(3):444-447). Among them, the composition of the trace element solution is: CuSO 4 ·5H 2 O 4.0g / L, FeSO 4 ·7H 2 O 0.7g / L, FeCl 3 ·6H 2 O 7.0g / L, CoCl 3 ·6H 2 O 0.2g / L, NaMO 4 2H 2 O 3.4g / L, CaCl 2 2H 2 O 2.0g / L.

[0023] LB medium: yeast extract 5g / L, peptone 10g / L, sodium chloride 5g / L.

[0024] Synthetic medium: add 50g / L glucose as carbon source to inorganic salt medium, and use different concentrations of dyes as nitrogen source.

[0025] Adjust the pH value of the medium to about 7.0; add...

Embodiment 2

[0034] The research of embodiment 2 HM01 bacterial strain decolorization, denitrification

[0035] (1) Decolorization and Denitrification Ability Determination

[0036] Inoculate the HM01 strain in LB medium for overnight culture at 30°C and 150rpm in a bed, centrifuge at 4000rpm, remove the supernatant, and resuspend with an equal volume of phosphate buffered saline (PBS) to obtain a seed solution, with a 10% inoculum size Inoculate it in a synthetic medium containing 20 mg / L reactive navy blue dye, culture it statically at 35°C, and take samples at regular intervals and store it at 4°C for testing.

[0037]1) Take 10mL of the sample to be tested in a centrifuge tube, centrifuge at 4000rpm for 10min and collect the supernatant liquid, resuspend the bacteria in the centrifuge tube with an equal volume of sterile water, and calculate the growth of the bacteria based on the light absorption value at 600nm. Ultraviolet-visible spectrophotometry (UV-Vis) was used to conduct full-...

Embodiment 3

[0044] The research of embodiment 3 HM01 bacterial strain decolorization optimal condition

[0045] (1) Effect of temperature on decolorization and degradation of HM01 strain

[0046] Under the condition that other reaction conditions are fixed, temperature is taken as the single factor variable, and the temperature conditions are respectively set as 25, 30, 35, 40, 45 and 50°C.

[0047] Strain HM01 was inoculated in LB culture medium at 30°C and 150rpm for overnight culture, centrifuged at 4000rpm, the supernatant was taken, and resuspended with an equal volume of phosphate buffered saline (PBS) to obtain a seed solution, which was inoculated at 10% inoculation volume In the synthetic medium containing 60mg / L reactive navy blue dye, it was cultured statically at different temperatures for 24 hours, and the decolorization and degradation of the dye by the HM01 strain were determined. see results Figure 5 , the optimum temperature for HM01 strain to degrade the dye is 35-40°...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
decolorization rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bacterial strain of Bacillus spp. for high-efficient wide-spectrum decolorization and degradation of reactive dyes and an application thereof, and belongs to the fields of microorganisms and waste water treatment. The bacterial strain is Bacillus spp. HM01 (Bacillus spp. HM01), and the preservation number is CCTCC No:M2017558. The bacterial strain can be used for decolorization and degradation of a plurality of reactive dyes and is used as a nitrogen source required by growth, can avoid nitrogen pollution caused by accumulation of nitrate nitrogen in a dye degradationprocess, and can be applied to treatment of printing and dyeing waste water containing a plurality of reactive dyes; the product has wide pH adaptation range, and has good theatrical research valuesand production application prospects.

Description

technical field [0001] The invention relates to the field of microorganisms and waste water treatment, in particular to a bacillus strain for efficient broad-spectrum decolorization and degradation of reactive dyes and an application thereof. Background technique [0002] Synthetic dyes have a wide variety, complex structure, good chemical stability, and poor biodegradability. They are the main pollutants in the textile industry and dye production wastewater. The resulting environmental pollution has always been a difficult point in industrial wastewater treatment. Since the advent of reactive dyes in 1956, after nearly 60 years of rapid development, they have gradually replaced sulfur dyes, vat dyes, direct dyes and ice dyes, and have become the main raw materials for printing and dyeing of cellulose fibers (including cotton, linen, rayon, etc.). However, in the process of dyeing with reactive dyes, about 30% of the reactive dyes cannot be effectively utilized and enter the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F103/34
CPCC02F3/34C02F2103/34C12N1/205C12R2001/07
Inventor 郑永良范媛媛喻玥许欢裴南妹徐碧林龙丽芳
Owner HUANGGANG NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com