Haematococcus pluvialis high-efficiency mariculture method
A technology of haematococcus pluvialis and mariculture, which is applied in the field of high-efficiency mariculture of haematococcus pluvialls, can solve the problem of low astaxanthin unit output, increase the accumulation of astaxanthin in single cells, and improve the reproductive capacity , The effect of promoting the accumulation of astaxanthin
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Embodiment 1
[0026] A high-efficiency seawater culture method for Haematococcus pluvialis, comprising the following steps:
[0027] S1: Media Configuration
[0028] The medium comprises the following components: glucose 3g / L, KNO 3 0.6g / L, NaH 2 PO 4 0.1g / L, MgSO 4 ·7H 2 O0.1g / L, Fe-EDTA1mg / L, citric acid 0.5mg / L, pumpkin seed powder 0.3g / L, sweet potato powder 0.2g / L, seawater 50mL / L, the pH value of this medium is 5.0;
[0029] S2: Growth stage of Haematococcus pluvialis
[0030] The algal species were inoculated into the culture medium with an inoculation amount of 17%, and irradiated with a mixed light source formed by a combination of red light, green light and blue light (the intensity ratio of red light, green light and blue light was 3:1:2). The light intensity was 2500Lx, the photoperiod was 12:12, the ambient temperature was controlled at 8°C, and carbon dioxide was introduced at a rate of 0.5 L / min per liter of medium, and the culture was cultivated for 2 days with continu...
Embodiment 2
[0034] A high-efficiency seawater culture method for Haematococcus pluvialis, comprising the following steps:
[0035] S1: Media Configuration
[0036] The medium comprises the following components: glucose 5g / L, KNO 3 1g / L, NaH 2 PO 4 0.3g / L, MgSO 4 ·7H 2 O0.2g / L, Fe-EDTA2mg / L, citric acid 1.0mg / L, pumpkin seed powder 0.7g / L, sweet potato powder 0.4g / L, seawater 100mL / L, the pH value of this medium is 6.0;
[0037] S2: Growth stage of Haematococcus pluvialis
[0038] The algal species were inoculated into the culture medium with an inoculation amount of 20%, and irradiated with a mixed light source formed by combining red light, green light and blue light (the intensity ratio of red light, green light and blue light was 3:1:2). The light intensity is 3000Lx, the photoperiod is 12:12, the ambient temperature is controlled at 15°C, and carbon dioxide is introduced at a rate of 0.7L / min per liter of medium, and the culture is cultivated for 3 days with constant stirring at...
Embodiment 3
[0042] A high-efficiency seawater culture method for Haematococcus pluvialis, comprising the following steps:
[0043] S1: Media Configuration
[0044] The medium comprises the following components: glucose 4g / L, KNO 3 0.7g / L, NaH 2 PO 4 0.2g / L, MgSO 4 ·7H 2 0.2g / L, Fe-EDTA2mg / L, citric acid 0.7mg / L, pumpkin seed powder 0.5g / L, sweet potato powder 0.3g / L, seawater 70mL / L, the pH value of this medium is 5.5;
[0045] S2: Growth stage of Haematococcus pluvialis
[0046] The algal species were inoculated into the culture medium with an inoculation amount of 20%, and irradiated with a mixed light source formed by combining red light, green light and blue light (the intensity ratio of red light, green light and blue light was 3:1:2). The light intensity is 3000Lx, the photoperiod is 12:12, the ambient temperature is controlled at 10°C, and carbon dioxide is introduced at a rate of 0.6L / min per liter of culture medium. Stir continuously at 170rpm and cultivate for 2 days. , the...
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