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Bacillus amyloliquefaciens and application thereof to prevention and treatment of autism

A technology of Bacillus amyloliquefaciens and autism, which is applied to a strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of autism. Reduce loneliness symptoms, good effect of application compliance

Inactive Publication Date: 2018-03-23
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cui Yunlong et al. disclosed in Patent No. 2016110603254 that Clostridium butyricum can treat autism and reduce the symptoms of autism, but there is no report that Bacillus amyloliquefaciens can prevent and treat autism.

Method used

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  • Bacillus amyloliquefaciens and application thereof to prevention and treatment of autism
  • Bacillus amyloliquefaciens and application thereof to prevention and treatment of autism
  • Bacillus amyloliquefaciens and application thereof to prevention and treatment of autism

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Experimental program
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Effect test

Embodiment 1

[0025] The separation of embodiment 1 bacillus amyloliquefaciens

[0026] In December 2014, the Bacillus amyloliquefaciens strain was isolated from the intestinal contents of wild wild boars in Tantou Town, Longhui County, Hunan Province. The specific method is: take the intestinal contents of wild wild boar and add it to a conical flask filled with 10mL of sterilized water, shake and incubate for 30min (200r / min), then take 1mL of the suspension and place it in 9mL of sterile water to make 10 -2 Dilute the suspension, and so on, to make 10 -5 , 10 -6 Dilute the suspension; draw 100u L each, add it to the nutrient broth medium plate, spread it evenly with a coating rod, and repeat each treatment 3 times. The above culture dish was placed in a constant temperature incubator at 37°C for 1-2 days. Select a single colony and transfer it to a nutrient broth plate for culture. After the colony grows, use an inoculation loop to streak and purify it, and store the purified strain ...

Embodiment 2

[0028] The preparation of embodiment 2 bacillus amyloliquefaciens preparation

[0029] 1. Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated with 5% inoculation amount into a 5L large triangle flask with a liquid volume of 2LLB medium, cultured with shaking at 33°C for 20-28 hours, and the concentration of the bacteria solution is tested. The viable bacteria content exceeds 2 billion / ml, then it is ready As a liquid strain of Bacillus amyloliquefaciens;

[0030] 2. Use the liquid Bacillus amyloliquefaciens cultured in step 1 as seeds, inoculate into 600L fermentation medium, the inoculation amount is 10%, stir at 120r / min, the air volume per minute liquid-gas ratio is 1:0.8, and cultivate at 33°C After 24-36 hours, when the spore con...

Embodiment 3

[0033] The safety test of embodiment 3 bacillus amyloliquefaciens

[0034] The Bacillus amyloliquefaciens liquid that the embodiment of the present invention 2 produces is through the rat acute toxicity test in Shaoyang Institute, and the conclusion result shows that the Bacillus amyloliquefaciens liquid is harmful to rats (feeding the Bacillus amyloliquefaciens liquid 14 of the present invention by 0.6ml / 10g body weight) day) Effect: All rats survived, moved freely, had smooth hair, normal diet, no secretions in the respiratory tract, eyes and mouth, no symptoms of poisoning were found, and their body weight increased. Compared with the control group of the blank group, the body weight of the rats fed with the Bacillus amyloliquefaciens solution increased significantly (P<0.001). At the end of the 14-day observation period, the rats were dislocated and dissected, and no obvious abnormalities were found in the heart, liver, spleen, lung, kidney, brain, intestine, small intesti...

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Abstract

The invention discloses bacillus amyloliquefaciens YHSH-58 ( / i) and application thereof to the prevention and treatment of autism. The bacillus amyloliquefaciens YHSH-58 ( / i) is preserved in China General Microbiological Culture Collection Center in No.3, 1 compound, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.13664, and the preservation date is February 16,2017.

Description

technical field [0001] The invention relates to microbial medicine and the application of a strain of bacillus amyloliquefaciens in the preparation of preparations for preventing or treating autism, specifically using bacillus amyloliquefaciens YHSH-58 to prevent or treat autism and reduce symptoms of autism. Background technique [0002] What is autism! Autism is a prevalent brain disorder. Note: it is a disorder, not a disease. [0003] How common is the disorder? Estimates of the prevalence of autism vary, depending on clinical criteria. Some estimates show that the prevalence of autism is as low as 5 cases per 10,000 people; however, some estimates show the prevalence as high as 1 case in 80 people. [0004] Individuals with autism - have three typical symptoms: poor social interaction, verbal and non-verbal communication difficulties, unusual or severely limited behaviors and interests. More specifically, people with autism may be more preoccupied with their own tho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/742A61P25/00C12R1/07
CPCA23V2002/00A61K2035/115A23L33/135C12N1/205C12R2001/07A23V2200/322
Inventor 蒋常德蒋常银
Owner FOSHAN YANHUI BIOTECH CO LTD
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