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Specific co-dominant molecular marker of brown planthopper resistance gene bph15 in rice and its application

A technology of co-dominant markers and brown planthoppers, applied in the field of genetic engineering, can solve the problems of complex identification of insect resistance, low efficiency of insect resistance gene transfer, and inability to identify individual plants, etc., and achieve the effect of clear location and easy detection

Active Publication Date: 2020-12-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the complexity of the identification of insect resistance of rice materials, in the backcross breeding of conventional insect-resistant genes, the group identification method at the seedling stage is generally used. Identification to determine contemporary pest resistance
Therefore, the transfer efficiency of insect-resistant genes by conventional breeding methods is low, and it is often difficult to effectively introduce or aggregate different insect-resistant genes

Method used

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  • Specific co-dominant molecular marker of brown planthopper resistance gene bph15 in rice and its application
  • Specific co-dominant molecular marker of brown planthopper resistance gene bph15 in rice and its application
  • Specific co-dominant molecular marker of brown planthopper resistance gene bph15 in rice and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Development and verification of rice brown planthopper resistance gene Bph15 specific co-dominant molecular marker

[0054] 1. Development of a specific co-dominant molecular marker for rice brown planthopper resistance gene Bph15

[0055] Standard Nucleotide BLAST analysis was performed on the genome sequence of the fine-mapped segment where Bph15 was located in the NCBI database ( https: / / blast.ncbi.nlm.nih.gov / Blast.cgi? PROGRAM= blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome ), compare the genome sequence of the fine-mapping section where Bph15 is located with the genome sequence of other rice varieties, and list the specific insertion / deletion fragments in the genome sequence of the Bph15 fine-mapping section; further use the Primer 3 online software to design and develop 7 easy-to-detect codominant molecular markers ( http: / / primer3.ut.ee / ).

[0056] Using the CTAB method (Murray MG & Thompson, 1980 Rapid isolation of high-molecular-weight plant...

Embodiment 2

[0064] Example 2 Application of specific co-dominant molecular marker of rice brown planthopper resistance gene Bph15 in molecular marker-assisted selection breeding

[0065] In traditional brown planthopper resistance breeding, the parents of brown planthopper-resistant materials are selected for hybridization with cultivars. It is necessary to identify the traits of brown planthopper resistance in the hybrid offspring for selection, and in view of the special circumstances of the identification of brown planthopper resistance, it can only be reflected by the identification of offspring materials The resistance phenotype of the individual plants of the material of the previous generation to N. lugens. Therefore, traditional breeding for resistance to BPH is not only time-consuming, but also difficult and costly. However, the application of molecular markers linked to BPH-resistant genes can identify BPH-resistant single plants at the seedling stage and eliminate other plants,...

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Abstract

The invention belongs to the field of genetic engineering and in particular discloses a specific codominant molecular marker for brown planthopper resistant genes Bph15 of rice and application thereof. By comparing genomic sequences in fine mapping intervals of the Bph15 with corresponding sequences of other rice varieties, specific codominant molecular markers B15InD1 and B15InD2 of the Bph15 aredeveloped and verified. According to the molecular marker disclosed by the invention, whether the brown planthopper resistant genes Bph15 of rice are contained in an insect-resistant variety B5 and derived varieties (lines) can be effectively detected, the selection efficiency of the brown planthopper resistant rice is greatly improved, and the brown planthopper resistant rice variety containingthe genes Bph15 is obtained. According to the codominant molecular marker provided by the invention, by virtue of molecular mark assisted breeding, a plurality of brown planthopper resistant genetic loci are gathered by virtue of gene pyramiding so as to obtain a high-quality broad spectrum resistant breeding material, the degradation age limit of the insect-resistant rice variety is delayed, andnew biotypes of the brown planthopper are prevented.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a specific co-dominant molecular marker of the rice brown planthopper resistance gene Bph15 and its application. Background technique [0002] Rice is one of the main food crops in my country, and its production is directly related to my country's food security, farmers' income increase and rural stability. Rice cultivation is faced with a wide variety of pests and diseases, and when the damage is serious, it poses a serious threat to its yield. Among them, the brown planthopper is an explosive and harmful rice pest. Both its adults and nymphs can directly suck the phloem juice of rice, resulting in yellow leaves and even death of plants. In addition, brown planthoppers can also transmit viruses and induce various rice diseases such as rice sawtooth dwarf disease and grass-like dwarf disease during the feeding process, which can lead to yield reduction or even cro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 何光存陈荣智杜波祝莉莉
Owner WUHAN UNIV
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