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Culturing method for wild lucid ganoderma strain

A technology of ganoderma strains and cultivation methods, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve problems such as not being able to meet the special requirements of strains, accelerate plant cell division, avoid repeated cultivation, and resist miscellaneous bacteria good performance

Inactive Publication Date: 2018-03-06
贺州市金子山生态灵芝林下种植专业合作社
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Strains are the basis of Ganoderma production, and the selection and breeding of excellent varieties is an important link to ensure the high-quality and high-yield of Ganoderma lucidum. The wild Ganoderma lucidum strains are refined, and the efficacy of Ganoderma lucidum obtained is higher than that of Ganoderma lucidum cultivated in greenhouses. However, wild Ganoderma lucidum collected from nature Although it can also be artificially cultivated, it cannot meet the special requirements for industrialized large-scale production of Ganoderma lucidum products, including: fermentation conditions, medium, temperature, humidity, and its synthesis and transformation of substrates during the fermentation process. Physiological and biochemical changes such as degradation

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  • Culturing method for wild lucid ganoderma strain

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Effect test

Embodiment 1

[0026] The cultivation method of a kind of wild Ganoderma lucidum strain provided by the present embodiment comprises the following steps:

[0027] (1) Preparation of tissue culture medium

[0028] The tissue culture medium is composed of the following raw materials in parts by weight: 30 parts of bean cake, 15 parts of rice bran, 10 parts of bran, 10 parts of sucrose, 20 parts of gypsum powder, 10 parts of soybean straw, 3 parts of agar, 1 part of boric acid, and forchlorfenuron 5 parts, 5 parts of brassinosterol and 80 parts of water; the configuration of the tissue culture medium is to mix the above raw materials, adjust the pH of the tissue culture medium to 5.0 by boiling, and then sterilize at a temperature of 95°C for 25 hours. The tissue culture medium can be obtained for use;

[0029] (2) Acquisition of cultivated species

[0030] Find the wild Ganoderma lucidum from the deep mountains, take the young stipe or the fruiting body of the growing cap that is robust, fre...

Embodiment 2

[0036] The cultivation method of a kind of wild Ganoderma lucidum strain provided by the present embodiment comprises the following steps:

[0037] (1) Preparation of tissue culture medium

[0038] The tissue culture medium is composed of the following raw materials in parts by weight: 35 parts of bean cake, 20 parts of rice bran, 12 parts of bran, 12 parts of sucrose, 22 parts of gypsum powder, 12 parts of soybean straw, 6 parts of agar, 3 parts of boric acid, and forchlorfenuron 7 parts, 7 parts of brassinosterol and 90 parts of water; the configuration of the tissue culture medium is to mix the above raw materials, adjust the pH of the tissue culture medium to 5.5 by boiling, and then sterilize at a temperature of 100°C for 30 hours. The tissue culture medium can be obtained for use;

[0039] (2) Acquisition of cultivated species

[0040] Find the wild Ganoderma lucidum from the deep mountains, take the young stipe or the fruiting body of the growing cap that is robust, f...

Embodiment 3

[0046] The cultivation method of a kind of wild Ganoderma lucidum strain provided by the present embodiment comprises the following steps:

[0047] (1) Preparation of tissue culture medium

[0048] The tissue culture medium is composed of the following raw materials in parts by weight: 40 parts of bean cake, 25 parts of rice bran, 15 parts of bran, 15 parts of sucrose, 25 parts of gypsum powder, 15 parts of soybean straw, 8 parts of agar, 5 parts of boric acid, and forchlorfenuron 9 parts, 10 parts of brassinosterol and 100 parts of water; the configuration of the tissue culture medium is to mix the above raw materials, adjust the pH of the tissue culture medium to 6.0 by boiling, and then sterilize at a temperature of 110°C for 35 hours. The tissue culture medium can be obtained for use;

[0049] (2) Acquisition of cultivated species

[0050] Find the wild Ganoderma lucidum from the deep mountains, take the young stipe or the fruiting body of the growing cap that is robust,...

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Abstract

The invention provides a method for cultivating wild ganoderma strains, which relates to the technical field of mushroom cultivation. The method for cultivating wild ganoderma strains comprises the following steps: (1) preparation of tissue culture medium, (2) obtaining cultivated species, (3) ) the cultivation of test tube species, (4) the cultivation of wild Ganoderma lucidum bacterial classification; the present invention is by selecting wild Ganoderma lucidum as cultivated species, and then cultivated species obtains test tube species through test tube cultivation, and then transforms into original species in the test tube, and then from original species to female Finally, the mother bell is connected to the wood and cultivated at a constant temperature until it is covered with hyphae to obtain the cultivated species. The invention is very rigorous in the process from material selection to cultivation, avoiding repeated cultivation of cultivated strains, and improving the formula of the medium. The cultivation process is optimized, it is not easy to cause the degradation of the strain, the mycelium is strong, and the anti-miscellaneous bacteria performance is good. The strain cultivated by the method of the present invention has tenacious vitality and high medicinal value, which can lay a solid foundation for the high-quality and high-yield cultivation of Ganoderma lucidum. good foundation.

Description

【Technical field】 [0001] The invention relates to the technical field of mushroom cultivation, in particular to a method for cultivating wild ganoderma strains. 【Background technique】 [0002] Ganoderma lucidum, also known as red lucidum and Huanyangcao, is a fungus of the genus Ganoderma lucidum, composed of a cap and a stipe, with a fragrant smell and a bitter taste. Modern medicine has proved that Ganoderma lucidum contains a variety of physiologically active substances, which can regulate and enhance human immunity, and have a good synergistic therapeutic effect on neurasthenia, rheumatoid arthritis, coronary heart disease, and hypertension. Fatigue, beauty and beauty, anti-aging, prevention and treatment of various diseases. [0003] Strains are the basis of Ganoderma production, and the selection and breeding of excellent varieties is an important link to ensure the high-quality and high-yield of Ganoderma lucidum. The wild Ganoderma lucidum strains are refined, and t...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20
Inventor 赵正英
Owner 贺州市金子山生态灵芝林下种植专业合作社
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