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Saccharomyces boulardii composite lyoprotectant, and preparation method and application method thereof

The technology of freeze-drying protective agent and yeast is applied in the field of yeast product production, which can solve the problems of easy water absorption and dampness, low number of viable bacteria, low freeze-drying survival rate, etc., and achieves the effect of improving health care function.

Inactive Publication Date: 2018-03-02
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a Pauladic yeast compound freeze-drying protective agent and its preparation and application method that can significantly improve the freeze-dried survival rate of Pauladic yeast and the number of live bacteria per unit of bacterial powder, and solve the problem of Pauladic yeast Yeast powder has problems such as low freeze-drying survival rate, low number of viable bacteria, easy to absorb water and become damp during the freeze-drying process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The Pauladic yeast compound lyoprotectant of the present embodiment comprises: 2g of xylooligosaccharides, 5g of inulin, 8g of fructooligosaccharides, 0.4g of sodium bicarbonate, 4g of sodium glutamate, 20g of Lactose, 0.7g of sodium ascorbate and 100mL of distilled water.

[0019] Its preparation method is as follows:

[0020] 1) First, take 0.7 g of sodium ascorbate and dissolve it in 20 mL of 0.9% sterile saline, and then pass through a 0.22 μm filter membrane to filter and sterilize to obtain solution A;

[0021] 2) Next, dissolve 2g of xylooligosaccharides, 5g of inulin, 8g of fructooligosaccharides, 0.4g of sodium bicarbonate, 4g of sodium glutamate, and 20g of lactose in 80mL of distilled water, and then dissolve them in 100°C Cool to room temperature after sterilization to obtain solution B;

[0022] 3) Finally, mix solution A and solution B evenly to obtain Pauladic yeast complex lyoprotectant.

[0023] The application method of the Paulady saccharomyces com...

Embodiment 2

[0026] The Pauladic yeast complex lyoprotectant of the present embodiment comprises: 2g of xylooligosaccharides, 5g of inulin, 10g of fructooligosaccharides, 0.3g of sodium bicarbonate, 5g of sodium glutamate, 20g of Lactose, 0.8g of sodium ascorbate and 100mL of distilled water.

[0027] Its preparation method is as follows:

[0028] 1) First, take 0.8 g of sodium ascorbate and dissolve it in 20 mL of 0.9% sterile saline, and then pass through a 0.22 μm filter membrane to filter and sterilize to obtain solution A;

[0029] 2) Next, dissolve 2g of xylooligosaccharides, 5g of inulin, 10g of fructooligosaccharides, 0.3g of sodium bicarbonate, 5g of sodium glutamate, and 20g of lactose in 80mL of distilled water, and then in 113 ° C Cool to room temperature after sterilization to obtain solution B;

[0030] 3) Finally, mix solution A and solution B evenly to obtain Pauladic yeast complex lyoprotectant.

[0031] The application method of the Paulady yeast composite freeze-dryin...

Embodiment 3

[0034] The Pauladic yeast complex lyoprotectant of the present embodiment comprises: 3g of xylooligosaccharides, 8g of inulin, 10g of fructooligosaccharides, 0.6g of sodium bicarbonate, 5g of sodium glutamate, 22g of Lactose, 0.8g of sodium ascorbate and 100mL of distilled water.

[0035] Its preparation method is as follows:

[0036] 1) First, take 0.8 g of sodium ascorbate and dissolve it in 20 mL of 0.9% sterile saline, and then pass through a 0.22 μm filter membrane to filter and sterilize to obtain solution A;

[0037] 2) Next, dissolve 3g of xylooligosaccharides, 8g of inulin, 10g of fructooligosaccharides, 0.6g of sodium bicarbonate, 5g of sodium glutamate, and 22g of lactose in 80mL of distilled water, and then in 118 ° C Cool to room temperature after sterilization to obtain solution B;

[0038] 3) Finally, mix solution A and solution B evenly to obtain Pauladic yeast complex lyoprotectant.

[0039] The application method of the Paulady saccharomyces composite free...

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Abstract

The invention discloses a Saccharomyces boulardii composite lyoprotectant, and a preparation method and an application method thereof. The preparation method comprises the following steps: dissolvingsodium ascorbate in sterile saline solution, and carrying out filtration sterilization to obtain a solution A; dissolving polyxylose, inulin, fructooligosaccharide, sodium bicarbonate, sodium glutamate and lactose in distilled water, sterilizing the obtained solution, and cooling the sterilized solution to room temperature in order to obtain a solution B; and uniformly mixing the solution A with the solution B to obtain the Saccharomyces boulardii composite lyoprotectant. The application method comprises the following steps: uniformly mixing Saccharomyces boulardii with the Saccharomyces boulardii composite lyoprotectant according to a mass ratio of 1:2, carrying out balancing for 40-60 min, and freezing the obtained mixture in a vacuum lyophilizer to obtain Saccharomyces boulardii powder.The Saccharomyces boulardii composite lyoprotectant is added in the probiotic powder preparation process to lyophilize the Saccharomyces boulardii, so the lyophilized survival rate can reach 80.23-86.26%, the number of living probiotics of the probiotic powder is 1.84-2.13 * 10<10> CFU / g, and the key technical problems in the development process of high-activity Saccharomyces boulardii lyophilized powder are solved.

Description

technical field [0001] The invention belongs to the technical field of yeast product production, and relates to a Pauladic yeast composite freeze-drying protectant for improving the freeze-dried survival rate of Pauladic yeast and a preparation and application method thereof. Background technique [0002] Saccharomyces boulardii is a yeast probiotic isolated from Indonesian lychee and other fruits by French scientist Henri Boulard in 1920. It is a non-pathogenic yeast, which is harmful to humans and animals. No toxic side effects. Saccharomyces cerevisiae is a single-celled fungus and a subspecies of Saccharomyces cerevisiae. It is heat-resistant and can grow at 37°C. [0003] At present, foreign countries have developed preparations for the treatment of diarrhea using Pauladic yeast, and are widely used in animal husbandry and human anti-diarrhea drugs. It can effectively reduce the concentration of pathogenic bacteria and related toxins, stimulate the immune system, and i...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/04C12R1/85
CPCC12N1/04C12N1/16
Inventor 陈合雷张腾姚春旭洪海平舒国伟
Owner SHAANXI UNIV OF SCI & TECH
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