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Preparation method of parathyroid hormone detection kit

A parathyroid hormone and detection kit technology, which is applied in the field of medical testing, can solve the problems of cumbersome operation of enzyme-linked immunosorbent assay, poor quantitative detection effect, radioactive pollution, etc., and achieves improved sensitivity and linear range, and low cost. , without the effect of radioactive contamination

Inactive Publication Date: 2018-02-27
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the enzyme-linked immunosorbent assay is cumbersome to operate, the quantitative detection effect is not good, and the results are greatly affected by human factors; the radioimmunoassay method has radioactive pollution, and the required equipment is relatively expensive

Method used

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  • Preparation method of parathyroid hormone detection kit
  • Preparation method of parathyroid hormone detection kit
  • Preparation method of parathyroid hormone detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation method of a kind of parathyroid hormone detection kit of the present embodiment comprises the following steps:

[0038] (1) Prepare reagent R1 according to the following component contents:

[0039] Reagent R1:

[0040]

[0041] ① According to the component content of the above reagent R1, dissolve MOPSO in purified water, stir evenly, adjust the pH, and prepare R1 buffer solution;

[0042] ②According to the component content of the above reagent R1, NaCl, NaN 3 , Gum Arabic, Tween-80, and PEG-2000 were dissolved in the R1 buffer, stirred evenly, and the reagent R1 was prepared after all the raw materials were fully dissolved;

[0043] (2) Prepare reagent R2 according to the following component contents:

[0044] Reagent R2:

[0045]

[0046] ① According to the component content of the above reagent R2, dissolve MOPSO in purified water, stir evenly, adjust the pH, and prepare R2 buffer solution;

[0047] ②According to the component content of ...

Embodiment 2

[0054] The preparation method of a kind of parathyroid hormone detection kit of the present embodiment comprises the following steps:

[0055] (1) Prepare reagent R1 according to the following component contents:

[0056] Reagent R1:

[0057]

[0058]

[0059] ① According to the component content of the above reagent R1, dissolve MOPSO in purified water, stir evenly, adjust the pH, and prepare R1 buffer solution;

[0060] ②According to the component content of the above reagent R1, NaCl, NaN 3, Gum Arabic, Tween-80, and PEG-2000 were dissolved in the R1 buffer, stirred evenly, and the reagent R1 was prepared after all the raw materials were fully dissolved;

[0061] (2) Prepare reagent R2 according to the following component contents:

[0062] Reagent R2:

[0063]

[0064] ① According to the component content of the above reagent R2, dissolve MOPSO in purified water, stir evenly, adjust the pH, and prepare R2 buffer solution;

[0065] ②According to the component ...

Embodiment 3

[0072] The preparation method of a kind of parathyroid hormone detection kit of the present embodiment comprises the following steps:

[0073] (1) Prepare reagent R1 according to the following component contents:

[0074] Reagent R1:

[0075]

[0076] ① According to the component content of the above reagent R1, dissolve MOPSO in purified water, stir evenly, adjust the pH, and prepare R1 buffer solution;

[0077] ②According to the component content of the above reagent R1, NaCl, NaN 3 , Gum Arabic, Tween-80, and PEG-2000 were dissolved in the R1 buffer, stirred evenly, and the reagent R1 was prepared after all the raw materials were fully dissolved;

[0078] (2) Prepare reagent R2 according to the following component contents:

[0079] Reagent R2:

[0080]

[0081] ① According to the component content of the above reagent R2, dissolve MOPSO in purified water, stir evenly, adjust the pH, and prepare R2 buffer solution;

[0082] ②According to the component content of ...

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Abstract

The invention discloses a preparation method of a parathyroid hormone detection kit. The preparation method comprises the following steps: (1) according to the component content of a reagent R1, dissolving MOPSO (3-(N-Morpholino)2-hydroxypropanesulfonic acid) into purified water and regulating the pH value, so as to obtain R1 buffer solution; dissolving NaCl, NaN3, Arabic gum, Tween-80 and PEG-2,000 into the R1 buffer solution, so as to obtain the reagent R1; (2) according to the component content of a reagent R2, dissolving MOPSO into purified water, so as to obtain an R2 buffer solution; dissolving NaCl, NaN3, BSA and glycerol into the R2 buffer solution, so as to obtain an R2 dispersion solution; preparing a latex coated parathyroid gland polyclonal antibody; dissolving the latex coatedparathyroid gland polyclonal antibody with the R2 dispersion solution, so as to obtain the reagent R2. The preparation method has the advantages that the operation is simple and fast, and the quantitative determination is accurate; the kit is high in sensitivity and wide in linear range; the kit is good in stability and strong in specificity; the kit is low in cost and is pollution-free and is used for a fully automatic biochemical analyzer.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a preparation method of a parathyroid hormone detection kit. Background technique [0002] Parathyroid hormone (parathyroid hormone) is a basic single-chain polypeptide hormone secreted by parathyroid chief cells, referred to as PTH. Parathyroid hormone is composed of 84 amino acids, and its main function is to regulate the metabolism of calcium and phosphorus in vertebrates, so as to promote the increase of blood calcium level and the decrease of blood phosphorus level. [0003] The first precursor substance of PTH is first synthesized in the chief cell of the parathyroid gland, called preproparathyroid hormone, which contains 115 amino acids, and then this precursor substance is cleaved in the cell to become the second precursor substance thyroid containing 90 amino acids. Proparahormone, which in turn is cleaved intracellularly into a polypeptide containing 84 amino a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/531
CPCG01N33/78G01N33/531G01N2333/635
Inventor 吴铮丁先骏吴泽东庄庆华张金东
Owner ANHUI IPROCOM BIOTECH CO LTD
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