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Preparation method of s100beta protein detection kit

A detection kit and β protein technology, applied in the field of medical testing, can solve the problems of complicated operation, low accuracy and high cost, and achieve the effects of low operating cost, good stability and simple operation.

Inactive Publication Date: 2018-02-27
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the products used for S100β protein detection on the market are mainly detected by fluorescence immunoassay and enzyme-linked immunoassay. These methods are high in cost and low in accuracy, and need to be equipped with specific instruments for detection, and the operation is cumbersome.

Method used

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  • Preparation method of s100beta protein detection kit
  • Preparation method of s100beta protein detection kit
  • Preparation method of s100beta protein detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of a kind of s100β protein detection kit of the present embodiment comprises the following steps:

[0035] (1) Prepare reagent R1 according to the following component contents:

[0036] Reagent R1:

[0037]

[0038] ① According to the component content of the above reagent R1, dissolve bovine serum albumin in boric acid buffer solution, stir evenly, wait until fully dissolved, and prepare R1 dispersion;

[0039] ② Dissolve PEG-6000, sucrose, trehalose, and HY-500 preservative in the R1 dispersion according to the component content of the above reagent R1, stir evenly, and wait until all the raw materials are fully dissolved to prepare the reagent R1;

[0040] (2) Prepare reagent R2 according to the following component contents:

[0041] Reagent R2:

[0042]

[0043] ① According to the component content of the above reagent R2, dissolve bovine serum albumin in boric acid buffer solution, stir evenly, wait for fully dissolved, and prepare R...

Embodiment 2

[0051] The preparation method of a kind of s100β protein detection kit of the present embodiment comprises the following steps:

[0052] (1) Prepare reagent R1 according to the following component contents:

[0053] Reagent R1:

[0054]

[0055] ① According to the component content of the above reagent R1, dissolve bovine serum albumin in boric acid buffer solution, stir evenly, wait until fully dissolved, and prepare R1 dispersion;

[0056] ② Dissolve PEG-6000, sucrose, trehalose, and HY-500 preservative in the R1 dispersion according to the component content of the above reagent R1, stir evenly, and wait until all the raw materials are fully dissolved to prepare the reagent R1;

[0057] (2) Prepare reagent R2 according to the following component contents:

[0058] Reagent R2:

[0059]

[0060] ① According to the component content of the above reagent R2, dissolve bovine serum albumin in boric acid buffer solution, stir evenly, wait for fully dissolved, and prepare R2 ...

Embodiment 3

[0068] The preparation method of a kind of s100β protein detection kit of the present embodiment comprises the following steps:

[0069] (1) Prepare reagent R1 according to the following component contents:

[0070] Reagent R1:

[0071]

[0072]

[0073] ① According to the component content of the above reagent R1, dissolve bovine serum albumin in boric acid buffer solution, stir evenly, wait until fully dissolved, and prepare R1 dispersion;

[0074] ② Dissolve PEG-6000, sucrose, trehalose, and HY-500 preservative in the R1 dispersion according to the component content of the above reagent R1, stir evenly, and wait until all the raw materials are fully dissolved to prepare the reagent R1;

[0075] (2) Prepare reagent R2 according to the following component contents:

[0076] Reagent R2:

[0077]

[0078] ① According to the component content of the above reagent R2, dissolve bovine serum albumin in boric acid buffer solution, stir evenly, wait for fully dissolved, a...

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Abstract

The invention discloses a preparation method of an s100beta protein detection kit. The preparation method comprises the following steps: (1), according to the component content of a reagent R1, dissolving bovine serum albumin into a boric acid buffer solution to obtain a R1 dispersion liquid, and dissolving PEG-6000, sucrose, trehalose and an HY-500 preservative into the R1 dispersion liquid to obtain the reagent R1; (2), according to the component content of a reagent R2, dissolving bovine serum albumin into the boric acid buffer solution to obtain a R2 primary dispersion liquid, and adding S9, the HY-500 preservative, latex microspheres and PVP into the R2 primary dispersion liquid to obtain a R2 dispersion liquid; performing an S100beta protein antibody labeling process; dissolving S100beta protein antibody latex particles with the R2 dispersion liquid, and performing ultrasonic dispersion to obtain the reagent R2. The preparation method has the advantages as follows: the prepared kit has high sensitivity and stability, and the operation is simple and fast.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a preparation method of an s100β protein detection kit. Background technique [0002] The s100β protein is also called the central nervous system-specific protein, and some scholars describe the s100β protein as the "C-reactive protein" of the brain. A certain physiological concentration of s100β protein has a neurotrophic effect, and a high concentration of s100β protein has neurotoxicity. Overexpression of S100β protein will increase the susceptibility of the brain to ischemia and hypoxia, leading to neuronal apoptosis. [0003] At present, the products used for S100β protein detection on the market are mainly detected by fluorescence immunoassay and enzyme-linked immunoassay. These methods are high in cost and low in accuracy, and need to be equipped with specific instruments for detection, and the operation is cumbersome. . [0004] Therefore, there is an urgent nee...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/4737G01N2800/28
Inventor 庄庆华吴泽东张金东吴铮丁先骏
Owner ANHUI IPROCOM BIOTECH CO LTD
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