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Method for determining residual quantity of pesticide enantiomers in tea leaves

A technology of pesticide residues and enantiomers, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor separation of enantiomers and long time consumption, so as to avoid the use of a large number of toxic reagents and time-consuming Short, good separation effect

Active Publication Date: 2018-02-23
北京市食品安全监控和风险评估中心(北京市食品检验所)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to overcome the separation and detection methods such as gas phase, liquid phase chromatography or capillary electrophoresis in the prior art. In order to provide a fast, simultaneous and efficient method for the determination of methamidophos and / or its derivatives chiral pesticide enantiomer residues in tea

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  • Method for determining residual quantity of pesticide enantiomers in tea leaves
  • Method for determining residual quantity of pesticide enantiomers in tea leaves
  • Method for determining residual quantity of pesticide enantiomers in tea leaves

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no. 1 approach

[0053] The first embodiment of the present invention provides a method for detecting the content of methamidophos and / or its derivative enantiomer from tea leaves containing pesticide residues. The method comprises the steps of:

[0054] Step 1: a step of providing tea raw materials containing residual methamidophos and / or derivatives thereof;

[0055] Step 2: extracting methamidophos and / or derivatives thereof remaining in the tea raw material described in step 1 and removing impurities other than methamidophos and / or derivatives thereof;

[0056] Step 3: performing chromatographic separation on the extract of methamidophos and / or its derivatives obtained in step 2 from which impurities have been removed, so that the enantiomers of methamidophos and / or its derivatives are separated;

[0057] Step 4: Perform mass spectrometry detection on the separated enantiomers obtained in step 3, so as to determine the content of each enantiomer.

[0058] Wherein, in step 3, the chromato...

no. 2 approach

[0102] A second embodiment of the present invention provides a method for separating the enantiomers of methamidophos and / or acephate. Described separation method comprises the steps:

[0103] A solution containing methamidophos and / or acephate enantiomers is separated using a chromatographic column,

[0104] The chromatographic column is a chiral chromatographic column, and the chiral chromatographic column includes a chiral stationary phase and a mobile phase,

[0105] The chiral stationary phase includes a matrix and cellulose or derivatives thereof modified by chiral substances on the surface of the matrix, and the chiral substances include phenylcarbamate or benzoic acid or derivatives thereof,

[0106] Described mobile phase comprises supercritical fluid (supercritical carbon dioxide) and modifier, and described modifier is selected from C 1 -C 4 Alcohol or nitrile compounds, preferably methanol, ethanol, isopropanol or acetonitrile,

[0107] The resolution of the me...

no. 3 approach

[0111] In the third embodiment of the present invention, a method for detecting the content of methamidophos and / or acetylmethamidophos enantiomers in pesticide residues is provided. The detection method includes the method for separating the enantiomers of methamidophos and / or acephate according to the second embodiment above.

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Abstract

The invention provides a method for determining the residual quantity of pesticide enantiomers in tea leaves. The determination method comprises the following steps: 1, providing a tea leaf raw material containing residual methamidophos and / or derivatives thereof; 2, extracting the methamidophos and / or derivatives thereof in the tea leaf raw material in step 1, and removing impurities except the methamidophos and / or derivatives thereof; 3, carrying out chromatographic separation on the obtained impurity-removed methamidophos and / or derivative extract to separate the enantiomers of the methamidophos and / or derivatives thereof; and 4, carrying out mass spectrometric detection on the separated enantiomers obtained in step 3 to determine the content of every enantiomer. The determination method can split single monomers or multiple compound enantiomers simultaneously, the resolution of the obtained enantiomers is greater than 1.5, and the time consumed in the detection process is short.

Description

technical field [0001] The invention relates to a method for determining the residual amount of pesticide enantiomers in tea, in particular to a chiral pesticide (+)-methamidophos, (-)-methamidophos, (+)-acetyl formazan in tea The invention discloses a method for determining residues of amidophos and (-)-acetylmethamidophos, belonging to the detection field of pesticide residues. Background technique [0002] Chirality refers to the three-dimensional space structure of the substance itself, which is symmetrical and mirror image, just like the left and right hands of a human being, which are similar but cannot overlap. It is one of the essential properties of nature. Constrained by conditions such as production technology, cost and actual use, nearly 25% of commercial pesticides in the world have enantiomers, and the proportion in China is estimated to exceed 40%. In pesticides, chiral enantiomers often exhibit different biological activities, and while one enantiomer has hi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N2030/062G01N2030/146
Inventor 郝杰姜洁罗晓轩吴燕涛史娜邵瑞婷张丽华周瑞泽
Owner 北京市食品安全监控和风险评估中心(北京市食品检验所)
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