Fifteen channel micro fluidic chip device for combined detection of multiple tumor markers

Abstract

The invention relates to a microfluidic chip device for joint detection of multiple tumor markers with fifteen channels, belonging to the field of analysis and testing. PDMS, that is, polydimethylsiloxane, has obvious advantages if it is used to make the substrate of a microfluidic chip for the joint detection of more than ten kinds of tumor markers, but there are also some problems that need to be overcome; problem. The main point of this case is that the PDMS with the original ecological surface is selected as the substrate, and the elastic clamp with the micro-ultrasonic transducer attached to the clamping arm is elastically positioned on the sample liquid flow terminal of the microfluidic chip and its neighbors. position, using ultrasonic waves to reduce interfacial tension and increase interfacial compatibility. At the same time, the strong absorption ability of PDMS to ultrasonic waves can be used to achieve a rapid decrease in ultrasonic intensity in a short distance, thereby forming a difference in interfacial tension at both ends of the chip, thereby facilitating the test. The sample liquid flows toward the terminal along the originally hydrophobic capillary channel. The device does not require the use of micropumps.

Description

technical field [0001] The invention relates to a microfluidic chip device for joint detection of multiple tumor markers with fifteen channels, belonging to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive syste...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the microchannel modified by the surface modification unable to maintain for a long enough time, and the microgroove after surface modification The maintenance time of the inner surface state of the channel is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the inner surface of the PDMS microchannel after surface modification or surface modification is formed after modification The surface state of the surface does not last long, but quickly tends to or changes back to the surface state before the surface modification, and returns to the original strongly hydrophobic surface state in a relatively short period of time. Then, just imagine, Can such microfluidic chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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