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Preparation method and application of cancer marker detecting system based on silver aggregate-polymer and gold nanowire array

A technology of gold nanowires and polymers, which is applied in the detection field of cancer markers, can solve the problems of low immune detection limit and achieve the effect of improving stability

Active Publication Date: 2018-01-30
赛尔医学科技(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a research hotspot and difficulty in the field of surface-enhanced Raman scattering (SERS)-based immunoassay is how to further obtain extremely high-intensity signal output, and then obtain a lower immunodetection limit

Method used

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  • Preparation method and application of cancer marker detecting system based on silver aggregate-polymer and gold nanowire array
  • Preparation method and application of cancer marker detecting system based on silver aggregate-polymer and gold nanowire array
  • Preparation method and application of cancer marker detecting system based on silver aggregate-polymer and gold nanowire array

Examples

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Embodiment 1

[0036] The preparation method of the prostate-specific antigen (PSA) detection system based on silver aggregate-polymer and gold nanowire array, comprises the following steps:

[0037] 1. Preparation of silver aggregate-polymer SERS immunoprobe

[0038] 1.47 milligrams of sodium citrate and 1.97 milligrams of chloroauric acid were dissolved in 20 milliliters of water to make a mixed solution, and 0.6 milliliters of freshly prepared sodium borohydride aqueous solution (concentration of 4 mg / ml) was added while vigorously stirring at room temperature to obtain gold seeds solution;

[0039] Get 3 milliliters of golden seed solution and join in 50 milliliters containing the aqueous solution of silver nitrate (concentration is 0.2 mg / ml) and sodium citrate (concentration is 0.6 mg / ml), then add 10 milliliters of ascorbic acid aqueous solution (concentration is 0.6 mg / ml) dropwise wherein 2 mg / ml), and reacted for 1 hour to obtain an aqueous solution of silver nanoparticles; take 1...

Embodiment 2

[0053] The preparation method of the alpha-fetoprotein antigen detection system based on silver aggregate-polymer and gold nanowire array, comprises the following steps:

[0054] 1. Preparation of silver aggregate-polymer SERS immunoprobe: Dissolve 2.94 mg of sodium citrate and 3.94 mg of chloroauric acid in 40 ml of water to make a mixed solution, add 1.2 ml of freshly prepared boron while vigorously stirring at room temperature Sodium hydride aqueous solution (concentration is 4 mg / ml) to obtain gold seed solution; get 6 milliliters of gold seed solution and add to 100 milliliters containing silver nitrate (concentration is 0.2 mg / ml) and sodium citrate (concentration is 0.6 mg / ml) In the aqueous solution of, add 20 milliliters of ascorbic acid aqueous solutions (concentration is 2 mg / ml) dropwise thereafter afterwards, react 1 hour to obtain silver nanoparticles aqueous solution;

[0055]Get 2 milliliters of silver nanoparticle aqueous solution and centrifuge and concentrat...

Embodiment 3

[0063] The preparation method of the carbohydrate antigen CA125 detection system based on the silver aggregate-polymer and the gold nanowire array comprises the following steps:

[0064] 1. Preparation of silver aggregate-polymer SERS immunoprobe

[0065] 4.41 milligrams of sodium citrate and 5.91 milligrams of chloroauric acid were dissolved in 60 milliliters of water to make a mixed solution, and 1.8 milliliters of freshly prepared sodium borohydride aqueous solution (concentration of 4 milligrams per milliliter) was added while vigorously stirring at room temperature to obtain gold seeds solution;

[0066] Get 9 milliliters of golden seed solution and join in 150 milliliters containing the aqueous solution of silver nitrate (concentration is 0.2 mg / ml) and sodium citrate (concentration is 0.6 mg / ml), then add 30 milliliters of ascorbic acid aqueous solution (concentration is 0.6 mg / ml) dropwise wherein 2 mg / ml), reacted for 1 hour to obtain an aqueous silver nanoparticle s...

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Abstract

The invention discloses a preparation method and application of a cancer marker detecting system based on a silver aggregate-polymer and a gold nanowire array. The preparation method is characterizedby comprising the following steps: (1) preparation of a silver aggregate-polymer SERS immunoprobe; (2) preparation of a gold nanowire array substrate; and (3) assembling of a cancer marker detecting system. According to the application of the cancer marker detecting system, the cancer marker detecting system based on the silver aggregate-polymer and the gold nanowire array is subjected to spectralmeasurement by a raman spectrometer, and the concentration of antigen in a phosphate buffered solution which contains to-be-measured antigen can be obtained by calculation according to the quantitative relation between the spectral intensity and the concentration of the antigen. The preparation method and application of the cancer marker detecting system based on the silver aggregate-polymer andthe gold nanowire array have the advantages that while the relatively low detection limit is obtained, the specificity and repeatability of a detection result are improved.

Description

technical field [0001] The invention relates to a method for detecting cancer markers, in particular to a preparation method and application of a cancer marker detection system based on silver aggregate-polymer and gold nanowire arrays. Background technique [0002] In recent years, with the continuous acceleration of the pace of human production and life, global warming, the sharp decline in biodiversity, and the problems of diseases caused by environmental damage such as regional air pollution have become more and more serious. Studies have shown that more than 70% of carcinogenic and cancer-promoting factors are closely related to environmental pollution. With the advancement of optoelectronic science and technology, especially the combination of micro-nano preparation technology, advanced photoelectric detection technology and modern biotechnology, people have developed biological agents for detecting cancer-specific antigens based on highly specific immune reactions bet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N21/65
Inventor 姜涛
Owner 赛尔医学科技(山东)有限公司
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