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Primer group for detecting nervous necrosis viruses of fishes and application thereof

A technology for nerve necrosis and primer sets, which can be applied in microorganisms, recombinant DNA technology, microorganism-based methods, etc., and can solve the problems of strict instrument requirements and prone to false positives.

Active Publication Date: 2018-01-30
FISHERIES RES INST OF FUJIAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the development of molecular biology, the PCR method is usually used to detect neuronecrosis virus. Although the sensitivity is high, the requirements for the instrument are strict, and false positives are prone to occur.

Method used

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  • Primer group for detecting nervous necrosis viruses of fishes and application thereof
  • Primer group for detecting nervous necrosis viruses of fishes and application thereof
  • Primer group for detecting nervous necrosis viruses of fishes and application thereof

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Embodiment 1

[0102] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0103] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. The following examples are just to illustrate the present invention, but not to limit the scope of the present invention in any way.

[0104] The invention designs primers for the coat gene sequence of nerve necrosis virus, and is used for detecting fish nerve necrosis virus, and the nerve necrosis virus is RGNNV serotype.

[0105] 1. Design and synthesis of primers

[0106] According to multiple fish neuronecrosis virus coat gene sequences, the sequence numbers are MF510920, EF558369.1, KM588181.1, KT071606.1, JF412257.1, JF412260.1, through https: / / www.ebi.ac.uk / Tools / msa / clustalo / website comparison, obtain a highly conserved sequence as shown in SEQ ID No:1,

[0107] SEQ ID No: 1

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Abstract

The invention discloses a primer group for detecting nervous necrosis viruses of fishes and application thereof, belongs to the technical field of biological detection and provides a primer group designed according to highly-conservative regions of Coat protein gene sequence of the nervous necrosis viruses of fishes. The primer group has the characteristics of high specificity and high sensitivity; the primer group is prepared into a kit for detecting nervous necrosis viruses of fishes based on the characteristics of the primer group; a loop-mediated isotherm amplification (LAMP) method is used for detecting nervous necrosis viruses of fishes, so the method has the characteristics of simplicity in operation, rapid reaction and visible reaction results.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a primer set for detecting fish nerve necrosis virus and an application thereof. Background technique [0002] Fish viral nerve necrosis (viral nervous necrosis, VNN) is a kind of fish epidemic infectious disease worldwide, which is extremely harmful to larvae and juvenile fish. In severe cases, the mortality rate can reach 100% within a week. Due to its high infectivity and harmfulness, it is listed as an important fish disease by the International Veterinary Organization (OIE) (OIE, 2001). At present, the disease is spreading rapidly in countries and regions other than Africa, and more than 40 species of fish have been infected, which has caused great harm to the mariculture industry of various countries. The causative agent of the infectious disease is nerve necrosis virus (Nervous necrosis virus, NNV), which belongs to the beta-nodavirus genus in the...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12R1/93
Inventor 葛辉林克冰黄种持朱志煌
Owner FISHERIES RES INST OF FUJIAN
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