Novel multi-stage resin separation and preparation process

Abstract

The invention relates to a novel multi-stage resin separation and preparation process. The separation and preparation process comprises the following steps: (1) ultrasonically dissolving and synthesizing heparin sodium raw materials with 20-30 times of aqueous solution; (2) loading samples with a non-linear method; (3) loading samples through a single column with a multi-column segmented high-pressure elution method; (4) obtaining a heparin sodium product with the purity greater than 98%, and testing with a HPLC method to confirm that the purity of the heparin sodium product is greater than 98%; (5) carrying out a column online activation and regeneration method. The novel multi-stage resin separation and preparation process has the following advantages: the novel multi-stage resin separation and preparation process adopts a macroporous adsorption resin material of PIPO-02 polystyrene, which not only has a lower cost than the C-18 material, about 20% of the cost of the C-18 material, and has 2.5 times of the absorption capacity of the C-18 material, but also improves the asymmetric chromatography concept with samples loaded with a single column; multi-column segmented high-pressureelution method is adopted to further improve the production efficiency and to achieve zero emission of harmful substances.

Description

technical field [0001] The invention belongs to the field of extraction, separation and purification of synthetic raw materials, and in particular relates to a novel multi-stage resin separation and preparation process. Background technique [0002] Heparin sodium is a mucopolysaccharide produced by mast cells of animal connective tissue. It is a natural anticoagulant substance. Treat thromboembolism, fulminant meningitis, sepsis, nephritis, acute myocardial infarction, arteriosclerosis and other diseases. It also has the functions of clarifying plasma lipids and lowering cholesterol. [0003] At present, heparin sodium is extracted from animal pig small intestine mucosa in the market. At present, the precipitation method or ion exchange method of long-chain pyridine compounds and long-chain quaternary ammonium salts are generally used to make crude products first, and then oxidize and refine them into finished products. This method has disadvantages such as inconvenient re...

AI Technical Summary

Problems solved by technology

This method has disadvantages such as inconvenient recovery of precipitant, high cost, long production cycle, and low yield; later, on this basis, a resin adsorption method was developed to extract heparin sodium. Heparin sodium with higher potency has been obtained, but there is still room for further research and development to obtain heparin sodium with higher potency