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Preparation method of probiotics micro-ecological medicine assisting in treatment of nasopharynx cancer

A technology of probiotic microecology and adjuvant therapy, applied in the field of microorganisms, can solve the problems of lack of microbial flora, etc., and achieve the effects of improving survival rate and quality of life, reducing mortality, and low price

Inactive Publication Date: 2018-01-19
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for preparing a probiotic microecological drug for the auxiliary treatment of nasopharyngeal carcinoma, so as to solve the lack of a microbial bacterium with definite curative effect on nasopharyngeal carcinoma in the prior art. group technical questions

Method used

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  • Preparation method of probiotics micro-ecological medicine assisting in treatment of nasopharynx cancer
  • Preparation method of probiotics micro-ecological medicine assisting in treatment of nasopharynx cancer
  • Preparation method of probiotics micro-ecological medicine assisting in treatment of nasopharynx cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A preparation method of probiotic microecological medicine for adjuvant treatment of nasopharyngeal carcinoma, comprising the following steps:

[0036] A. Culture and isolation of bacterial flora

[0037](1) Feces collection and treatment: collect the first morning feces samples from cancer-free villagers (Wuyuan Cancer-free Village), and immediately transfer them into sealed anaerobic plastic bags (85% N 2 , 5%H 2 , 10% CO 2 ) to stand for one hour, then weigh 10g of feces sample into a 10mL centrifuge tube with built-in sterilized glycerin, the glycerin content is 10% of the sample, and seal the centrifuge tube mouth with sealing glue. Quickly store the samples in a -80°C ultra-low temperature freezer.

[0038] (2) Isolation of intestinal microorganisms: Aseptically take 0.5-1.0 g of feces, add them to a test tube, add sterile glass beads to fully shake the feces samples, mix them well and perform 10-fold dilution, select the appropriate concentration for selective...

Embodiment 2

[0093] This example is used to investigate the therapeutic effect of the probiotic microecological drug prepared in Example 1 on nasopharyngeal carcinoma. 1. Preparation of probiotic compound yogurt

[0094] Take the individual strains of probiotics obtained through the screening of steps A, B, and C in Example 1, ferment them to make yogurt, and then investigate their therapeutic and recovery effects on small-scale nasopharyngeal cancer patients (4-6 people) by oral administration . After preliminarily judging its effectiveness, all effective probiotics were fermented into yogurt and stored in a 4-degree refrigerator to evaluate its therapeutic effect on patients with nasopharyngeal carcinoma.

[0095] 2. Patients with nasopharyngeal carcinoma were enrolled

[0096] Patients with nasopharyngeal carcinoma who were admitted to Jiangxi Cancer Hospital after January 2017 were selected, and 5-10 patients in each group were screened for radiotherapy and chemotherapy + probiotic a...

Embodiment 3

[0110] A preparation method of probiotic microecological medicine for adjuvant treatment of nasopharyngeal carcinoma, comprising the following steps:

[0111] 1) Take the feces of people who do not suffer from cancer in Yuantou Village, Fuchun Town, Wuyuan County, Jiangxi Province, China, put them in an anaerobic bag and let them stand for 1 hour, then take a feces sample from it and add it to a centrifuge containing sterilized glycerin. In the centrifuge tube, the weight of the stool sample in the centrifuge tube is 10% of the weight of glycerin, seal the centrifuge tube mouth and centrifuge, and then store the centrifuge tube in the environment of -80°C;

[0112] 2) Take 0.5g of the mixture from the centrifuge tube obtained in step 1) and add it to the test tube, add sterile glass beads to shake and mix it, and then make a 10-fold dilution with sterile water, take a sample from the diluted product, and use the selective The culture medium screens out Lactobacillus plantarum ...

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Abstract

The invention provides a preparation method of a probiotics micro-ecological medicine assisting in treatment of nasopharynx cancer. According to the technical scheme, probiotics are separated from theintestines of healthy people from a cancer-free village in Wuyuan; after identification, the acid resistance, the cholate resistance, the oxidation resistance and the antibacterial property of the probiotics are evaluated; strains with good adhesive ability to nasopharynx cancer cells are screened out by combining co-culture and microscopic examination observation. Based on this, the screened strains are compounded with conventional probiotics such as animal bifidobacteria, lactobacillus rhamnosus and lactobacillus acidophilus, and the content proportion and the viable bacteria concentrationare limited. According to the preparation method provided by the invention, the screened strains all come from the human intestines s, the bifidobacteria and the lactic acid bacteria are taken as mainaddition objects for compounding; theoretically, the strains are safe to human body and do not cause any toxic or side effect; according to the method, at least one strain of bifidobacteria with an exact curative effect on nasopharynx cancer patients is added, and the effectiveness of nasopharynx cancertreatment is ensured; the adopted probiotics are directly screened from the healthy people in the cancer-free village, and thus the medicine has the advantage of low price over other medicines.

Description

technical field [0001] The invention relates to the technical field of microorganisms, further relates to the physiological application of probiotics and the treatment technology of tumors, and in particular to a preparation method of a probiotic microecological drug for the auxiliary treatment of nasopharyngeal carcinoma. Background technique [0002] Nasopharyngeal carcinoma refers to malignant tumors that occur on the top and side walls of the nasopharyngeal cavity. It is one of the high-risk malignant tumors in my country. The average natural survival time of patients is only 18.7 months. The occurrence of nasopharyngeal carcinoma is closely related to Epstein-Barr virus infection, environment and diet, genetic factors and chemical factors. [0003] WHO statistics show that more than 80% of nasopharyngeal carcinoma cases in the world occur in China, and nasopharyngeal carcinoma is a high incidence tumor in southern my country. Radiation therapy is the main method of nas...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A61K35/747A61P35/00C12R1/25C12R1/225C12R1/01C12R1/23
Inventor 陈廷涛辛洪波孟凡景
Owner NANCHANG UNIV
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