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Application of myricetin in preparation of protective preparation resisting doxorubicin-induced cardiotoxicity

A cardiotoxic and anti-doxorubicin technology, applied in the field of pharmacy, can solve the problems affecting the physical and mental health of patients, myocardial cell damage, etc., and achieve the effects of high heat and chemical stability, strong scavenging ability, and resistance to chemical disturbances

Active Publication Date: 2018-01-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of clinical application, it has a strong damage effect on cardiomyocytes and produces doxorubicin cardiotoxicity, which will seriously affect the physical and mental health of patients

Method used

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  • Application of myricetin in preparation of protective preparation resisting doxorubicin-induced cardiotoxicity
  • Application of myricetin in preparation of protective preparation resisting doxorubicin-induced cardiotoxicity
  • Application of myricetin in preparation of protective preparation resisting doxorubicin-induced cardiotoxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The efficacy test of myricetin comprises the following steps:

[0014] (1) Culture of rat H9C2 cardiomyocytes: take the H9C2 cardiomyocytes in the logarithmic hyperplasia phase for subculture, discard the original culture medium in the culture dish, add about 2 mL of trypsin, and wait for the cells to digest for about 2 minutes. Observe its shape under a microscope. When the cell shape becomes round and bright, discard the digestion solution, add about 6mL of DMEM culture solution, blow the cells with a dropper to suspend them, and put the suspension containing H9C2 cells into 10mL In a centrifuge tube, centrifuge at a speed of 1000r / min for 5min, suck up the supernatant and discard it, add 8mL of new culture medium to resuspend the cells, divide them evenly into two new culture bottles, mark them and put them into 37°C, 5% CO 2 Culture in an incubator with saturated humidity. When the cell coverage rate is about 90%, replace the culture medium 1 day before freezing, a...

Embodiment 2

[0027] Preparation of anti-adriamycin cardiotoxicity protection preparation: the preparation is a microcapsule, and the preparation method is:

[0028] 1) Preparation of drug-loaded sericin microcapsules: take 6 parts of sericin solution with a mass fraction of 0.2%, add 9 parts of CaCl with a concentration of 60 mM 2 solution, add 0.1 part of ametin and 0.0025 part of myricetin, adjust the pH of the solution to 7, and react at a constant temperature at 37 ° C for 23 hours. Store at ℃;

[0029] 2) Preparation of silica / sericin hybrid microcapsules: Take 5 parts of sericin microcapsules, add 0.1 part of polyallylamine hydrochloride and 100 parts of ultrapure water to it, stir for 10 minutes, then add 5 parts Ammonia water with a mass fraction of 25%, stirred for 5 minutes, then added 3 parts of ethyl orthosilicate, 20 parts of absolute ethanol, 0.1 part of dodecylamine and 0.1 part of pinacol, reacted at 23 °C for 20 minutes, centrifuged, and Wash twice with ultra-pure water ...

Embodiment 3

[0031] Preparation of anti-adriamycin cardiotoxicity protection preparation: the preparation is a microcapsule, and the preparation method is:

[0032] 1) Preparation of drug-loaded sericin microcapsules: take 5 parts of sericin solution with a mass fraction of 0.18%, add 8 parts of CaCl with a concentration of 55mM 2 solution, then add 0.08 parts by mass fraction of ametin and 0.001 parts of myricetin, adjust the pH of the solution to 6, and react at a constant temperature of 34 ° C for 19 hours. Store at 5°C. The preparation method is simple, and the active components doxorubicin and myricetin are loaded in one step. The prepared drug-loaded sericin microcapsules have high drug loading rate and good biocompatibility. Eliminate environmental pollution and waste of resources;

[0033] 2) Preparation of silica / sericin hybrid microcapsules: Take 4 parts of sericin microcapsules, add 0.07 parts of polyallylamine hydrochloride and 90 parts of ultrapure water to it, stir for 10 m...

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Abstract

The invention discloses an application of myricetin in preparation of a protective preparation resisting doxorubicin-induced cardiotoxicity and also discloses protective microcapsules resisting doxorubicin-induced cardiotoxicity. The protective preparation resisting doxorubicin-induced cardiotoxicity has the beneficial effects as follows: the preparation has quite high oxygen free radical scavenging capability, lipid peroxidation of the myocardial cell membrane and damage of myocardial mitochondrial DNA are prevented, and apoptosis of myocardial cells can be obviously reduced; the prepared microcapsules have higher thermal and chemical stability, and can better resist chemical disturbance in biological microenvironments.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to the application of myricetin in preparing anti-adriamycin cardiotoxicity protection preparations. Background technique [0002] Doxorubicin is an anthracycline antitumor antibiotic, which can inhibit the synthesis of RNA and DNA. It has a broad antitumor spectrum and can kill tumor cells in various growth cycles. It is mainly applicable to acute and chronic leukemia, malignant lymphoma, breast cancer and other solid tumors. However, in the course of clinical application, it has a strong damage effect on cardiomyocytes and produces doxorubicin cardiotoxicity, which will seriously affect the physical and mental health of patients. Therefore, it is very necessary to find drugs that can reduce the cardiotoxicity of doxorubicin for its clinical application. Contents of the invention [0003] The object of the present invention is to use myricetin for preparing anti-adriamycin c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/04A61K47/42A61K31/352A61K31/704A61P35/00A61P39/06A61P9/00
Inventor 赵玉勤
Owner ZHEJIANG OCEAN UNIV
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