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Optic nerve failure based sepiella maindroni maturity promoting method and reagent

A technology of Mann's needle-free squid and optic nerve, applied in the field of aquaculture, can solve the problems of large-scale breeding and breeding difficulties of Mann's needle-free squid, and achieve the effects of high drug stability, increasing body weight, and promoting sexual maturity.

Inactive Publication Date: 2018-01-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method belongs to natural reproduction, which makes large-scale breeding and breeding of squid mansoni relatively difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for promoting the ripening of squid without needles based on optic nerve destruction, the specific steps are:

[0023] 1) Selectively mature individuals of Squid mansoni;

[0024] 2) Soak the squid in seawater containing 1% ethanol, 18g / L magnesium chloride and 3mg / L isoeugenol for 100s to deeply anesthetize the squid;

[0025] 3) Disinfect the squid, then insert a red-hot scalpel with an alcohol lamp into one eye, and rotate the knife edge around the eyeball to destroy the optic nerve connecting the optic lobe and the eyeball;

[0026] 4) After optic nerve resection, the eye socket should be closed with medical butyl cyanoacrylate tissue glue to avoid infection;

[0027] 5) Take out the squid, rinse it with clean water, soak it in an aqueous solution containing 1ug / L modafinil and 0.8ug / L stephine for 10 minutes, and then use a tap water pipe or faucet to repeatedly rinse the squid's coat cavity to promote its recovery;

[0028] 6) Put the squid in seawate...

Embodiment 2

[0034] The method for promoting the ripening of squid without needles based on optic nerve destruction, the specific steps are:

[0035] 1) Selectively mature individuals of Squid mansoni;

[0036] 2) Soak the squid in seawater containing 3% ethanol, 17g / L magnesium chloride and 6mg / L isoeugenol for 80s to deeply anesthetize the squid;

[0037] 3) Disinfect the squid, then insert a red-hot scalpel with an alcohol lamp into one eye, and rotate the knife edge around the eyeball to destroy the optic nerve connecting the optic lobe and the eyeball;

[0038] 4) After optic nerve resection, the eye socket should be closed with medical butyl cyanoacrylate tissue glue to avoid infection;

[0039] 5) Take out the squid, rinse it with clean water, soak it in an aqueous solution containing 2ug / L modafinil and 0.6ug / L stephine base for 20 minutes, and then use a tap water pipe or faucet to repeatedly wash the squid's coat cavity to promote its recovery. The weight ratio of L-stepherine ...

Embodiment 3

[0046] The method for promoting the ripening of squid without needles based on optic nerve destruction, the specific steps are:

[0047] 1) Selectively mature individuals of Squid mansoni;

[0048] 2) Soak the squid in seawater containing 2% ethanol, 17.5g / L magnesium chloride and 5mg / L isoeugenol for 90s to deeply anesthetize the squid;

[0049] 3) Disinfect the squid, then insert a red-hot scalpel with an alcohol lamp into one eye, and rotate the knife edge around the eyeball to destroy the optic nerve connecting the optic lobe and the eyeball;

[0050] 4) After optic nerve resection, the eye socket should be closed with medical butyl cyanoacrylate tissue glue to avoid infection;

[0051] 5) Take out the squid, rinse it with clean water, soak it in an aqueous solution containing 1.5ug / L modafinil and 0.7ug / L stephine for 15 minutes, and then use a tap water pipe or faucet to repeatedly rinse the squid's coat cavity to promote its recovery;

[0052] 6) Put the squid in seaw...

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Abstract

The invention discloses an optic nerve failure based sepiella maindroni maturity promoting method and reagent. The maturity promoting method includes: selecting sexually-mature sepiella maindroni individuals; subjecting a squid to deep anesthesia; using a scalpel to damage the optic nerve; closing eye sockets to avoid infection; performing anabiosis; placing the squid in seawater for a rest-cure and then feeding a maturity promoting reagent. The maturity promoting reagent is prepared from serotonin, chorionic gonadotropin, a luteinizing hormone releasing hormone analogue, a fish gonad extract,a pituitary extract and a slow releasing agent. The maturity promoting method and reagent has the advantages that the optic nerve of the squid is damaged leading to increasing of the gonad, the purpose of maturity promoting of the squid is achieved, simpleness in operation is achieved, and damage to the squid is minimized; the reagent is high in drug stability and can prevent stomach inactivationof drugs and reduce stimulation of the drugs to the stomach, and efficacy is improved.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method and a reagent for promoting ripening of squid mansoni based on optic nerve destruction. Background technique [0002] Sepiella maindroni belongs to Mollusca Mollusca, Cephalopoda, Dibranchia, Sepioidea, Sepiidae, Sepiella. Man's needleless squid is a seafood with high protein, low fat and comprehensive nutrition. Its economic value is high, and its edible portion accounts for 92% of the total. Its meat is white, nutritious, high-quality protein and rich in trace elements, and delicious. Regular consumption can delay intelligence, delay aging, improve hematopoietic and immune functions, and have a certain effect on preventing cardiovascular diseases, anemia and tumors. However, with the rounding up of spawning groups and the damage to juvenile squid caused by netting operations, the increase in fishing intensity has prevented the squid population from being effectiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61K38/24A61P15/08A61K31/4045A61K38/09A61K35/60A61K35/30
CPCY02A40/81
Inventor 刘立芹张瑶刘炳舰龚理吕振明
Owner ZHEJIANG OCEAN UNIV
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