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Method for synthesizing Artemisia sphaerocephala polysaccharide derivatives by adopting acidic ionic liquid as catalyst

A technology of acidic ionic liquid and polysaccharide derivatives is applied in the field of application of selenized Artemisia annua polysaccharide to inhibit H1650 lung cancer cells in vitro, and can solve the problems of unfavorable molecular weight and molecular weight distribution, complex preparation of selenium-containing intermediates, unfriendly environment and the like. , to achieve the effect of shortening the time required for the reaction, high yield, and reducing the acid hydrolysis reaction steps

Active Publication Date: 2018-01-16
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The polysaccharide selenization modification technologies involved in the above patents and documents all have their own shortcomings: as a selenization reagent, selenite (sodium) needs to be reacted in an acidic aqueous solution, which will affect the molecular weight of the esterification reaction and the product selenium polysaccharide. , the control of molecular weight distribution is unfavorable; the preparation of selenium polysaccharides by organic method, the preparation of selenium-containing intermediates is complex and toxic, with many side reactions, and the environment is not friendly

Method used

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  • Method for synthesizing Artemisia sphaerocephala polysaccharide derivatives by adopting acidic ionic liquid as catalyst
  • Method for synthesizing Artemisia sphaerocephala polysaccharide derivatives by adopting acidic ionic liquid as catalyst

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Embodiment 1

[0044] A kind of synthesis of polysaccharide of selenized Artemisia annua, comprising:

[0045] (1) Preparation of polysaccharide formamide solution: 300 mg polysaccharide polysaccharide was dissolved in 75 mL anhydrous formamide at 60°C and 800 rpm for 3 h to obtain the polysaccharide formamide solution.

[0046] (2) Preparation of acidic ionic liquid: Add 10 mL concentrated nitric acid dropwise to 9.86 g imidazole at a rate of 40 drops / min at -10°C. After completion, wash with 10 mL ethyl acetate for 5 times at room temperature, collect the lower layer, and vacuum-dry at 70 °C and 0.06 MPa for 9 h to obtain an acidic ionic liquid. H 0 is 3.78.

[0047] (3) Synthesis of polysaccharides from selenized Artemisia argyi: 0.75 g of selenous acid was added to 10 mL of acidic ionic liquid prepared in step (2), and the 2 Under the condition of protection, add the formamide solution of Artemisia polysaccharides in (1) dropwise at a rate of 80 drops / min with a dropping funnel. Afte...

Embodiment 2

[0050] A kind of synthesis of polysaccharide of selenized Artemisia annua, comprising:

[0051] (1) Preparation of polysaccharide formamide solution: 300 mg polysaccharide polysaccharide was dissolved in 150 mL anhydrous formamide at 70°C and 500 rpm for 6 h to obtain the polysaccharide formamide solution.

[0052] (2) Preparation of acidic ionic liquid: 10 mL of concentrated sulfuric acid was added dropwise to 15.1 g of methylimidazole at a rate of 50 drops / min at 0 °C. After the addition was completed, the reaction was stirred at room temperature at 700 rpm for 12 h. After the reaction was completed, it was washed three times with 15 mL of ethyl acetate at room temperature, and the lower layer was collected and vacuum-dried at 50 °C and 0.07 MPa for 12 h to obtain an acidic ionic liquid. H 0 is 3.76.

[0053] (3) Synthesis of polysaccharides from selenized Artemisia arvensis: 0.75 g of selenous acid was added to the acidic ionic liquid (12.5 mL) prepared in step (2), unde...

Embodiment 3

[0056] A kind of synthesis of polysaccharide of selenized Artemisia annua, comprising:

[0057] (1) Preparation of polysaccharide formamide solution: 300 mg polysaccharide polysaccharide was dissolved in 100 mL anhydrous formamide at 50°C and 600 rpm for 5 h to obtain the polysaccharide formamide solution.

[0058] (2) Preparation of acidic ionic liquid: Add 10 mL of concentrated hydrochloric acid dropwise to 8.16 g of imidazole at a rate of 60 drops / min at -5°C. After completion, wash with 10 mL of ethyl acetate four times at room temperature, collect the lower layer, and vacuum-dry at 60 °C and 0.08 MPa for 8 h to obtain an acidic ionic liquid. H 0 was 3.79.

[0059] (3) Synthesis of polysaccharides from selenized Artemisia arvensis: 0.75 g of selenous acid was added to the acidic ionic liquid (7.5 mL) prepared in step (2), and the 2 Under the condition of protection, add the formamide solution of Artemisia polysaccharides in (1) dropwise at a rate of 70 drops / min with a...

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Abstract

The invention discloses a method for synthesizing Artemisia sphaerocephala polysaccharide derivatives by adopting an acidic ionic liquid as a catalyst and an application of Artemisia sphaerocephala polysaccharides and belongs to the technical field of biopolymers. The acidic ionic liquid is adopted to catalyze seleninic acid for synthesis of the Artemisia sphaerocephala polysaccharides, and products have the characteristics of low molecular weight and high selenium content (10370-20438 mu g / g). The adopted reagent is environmentally friendly, preparation is simple, a toxic selenium-containingintermediate is not needed, reaction conditions are mild, yield is high, selenium content is increased effectively, time needed for the reaction is shortened, the capability of the Artemisia sphaerocephala polysaccharides for inhibiting lung cancer H1650 cells in vitro is improved substantially, and the application range of the Artemisia sphaerocephala polysaccharides as natural active substancesis expanded.

Description

technical field [0001] The invention belongs to the technical field of biological macromolecules, and relates to a method for synthesizing derivatives of Artemisia annua polysaccharides by using an acidic ionic liquid as a catalyst; the invention also relates to the application of selenized Artemisia arumensis polysaccharides to inhibit H1650 lung cancer cells in vitro. Background technique [0002] Selenium is an important micronutrient element for the human body. The chemical and biological research on selenium focuses on the discovery of the structure and function of various inorganic selenium and organic selenium compounds (especially selenoproteins and selenium polysaccharides). At present, people attach great importance to the enhancement (or change) of the activity of polysaccharides after selenization, mainly focusing on immune regulation in vivo and in vitro, anti-tumor and anti-oxidation. Selenium polysaccharides are currently reported to be prepared by chemical ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 王俊龙包爱娟杨文赵雅楠武强强孔维宝梁俊玉姚健张继
Owner NORTHWEST NORMAL UNIVERSITY
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