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Method for screening magnetic nanoparticle modified cells based on photo-induced dielectrophoresis device

A technology of light-induced dielectrophoresis and magnetic nanoparticles, applied in biochemical equipment and methods, electric/wave energy treatment of enzymes, electric/wave energy treatment of microorganisms, etc., can solve cell damage, poor accuracy of experimental results, red blood cell extraction Complicated problems, to achieve the effect of good accuracy, convenient optical path construction, and easy promotion and application

Active Publication Date: 2018-01-12
CHANGCHUN UNIV OF SCI & TECH
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Problems solved by technology

In addition, the extraction of red blood cells in human blood is more complicated and requires fluorescent labeling, which may cause damage to the cells, resulting in poor accuracy of experimental results

Method used

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  • Method for screening magnetic nanoparticle modified cells based on photo-induced dielectrophoresis device
  • Method for screening magnetic nanoparticle modified cells based on photo-induced dielectrophoresis device
  • Method for screening magnetic nanoparticle modified cells based on photo-induced dielectrophoresis device

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Embodiment Construction

[0025] Such as figure 1 Shown, the light-induced dielectrophoresis device that the present invention adopts comprises computer 11, projector 12, signal generator 13, optical microscope 14, optical microscope illumination source 15, charge-coupled device (CCD) 16, convex lens 17, mirror 18, Light-induced dielectrophoresis chip 19; further, connect the computer 11 to the projector 12, project the photoelectrode pattern through the optical system, that is, the convex lens 17, the mirror 18, and focus on the light-induced dielectrophoresis chip 19. The solution layer 24 of 19 generates a non-uniform electric field to realize cell manipulation and screening, and is connected to a charge-coupled device (CCD) 16 through an optical microscope illumination source 15, an optical microscope 14, and is projected on a computer 11 for real-time observation.

[0026] Such as figure 2 As shown, the light-induced dielectrophoretic chip 19 includes a glass plate 21 , an indium tin oxide condu...

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Abstract

The invention relates to a method for screening magnetic nanoparticle modified cells based on a photo-induced dielectrophoresis device. By virtue of the photo-induced dielectrophoresis device, the cells, which are modified by virtue of magnetic nanoparticles, are screened out from a mixed solution; a photo-induced dielectrophoresis light path is constructed; light, which is transmitted from a light source, is irradiated onto a chip through the light path; a non-uniform electric field is formed by a solution layer of the chip; and cell screening is implemented on the basis of differences between yeast cells carrying the magnetic nanoparticles and yeast cells. With the application of the method provided by the invention, homogeneous cells can be screened; and the method is relatively short in duration required by a screening process and is higher in accuracy of screening the particles.

Description

technical field [0001] The invention belongs to the field of micro-nano control, and in particular relates to a method for screening cells modified by magnetic nanoparticles based on a light-induced dielectrophoresis device. Background technique [0002] In the field of manipulation of micro-nano biological particles, with the continuous development of micro-processing technology, many labs on a chip have emerged. The manipulation and separation of micro-nano biological particles has attracted extensive attention from many scientists. Many biological particles will have a certain dielectric In the case of an external electric field, these biological particles will be polarized to different degrees. If the external electric field is a non-uniform electric field, a dielectrophoretic force will be generated to make the particles move under the action of force. In recent years, dielectrophoresis technology has been introduced into the field of biological particle manipulation, a...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N1/16
Inventor 王作斌陈思兰杨焕洲刘梦楠董莉彤郝博王莹刘劲芸邸旭宋正勋翁占坤许红梅
Owner CHANGCHUN UNIV OF SCI & TECH
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