Culture medium for producing D-pantolactone hydrolase by fermentation through fusarium oxysporum mycete

Abstract

The invention relates to a culture medium for producing D-pantolactone hydrolase by fermentation through fusarium oxysporum mycete. The culture medium for producing D-pantolactone hydrolase by fermentation through fusarium oxysporum mycete comprises a seed culture medium and a fermentation culture medium; phytic acid is used in the culture medium, the activity of strains is improved, growth and metabolism of mycelia are promoted, the quality of a seed solution is improved, and the fermentative enzyme production capacity is improved; and furfural is used in the fermentation culture medium, fermentation is induced favorably to generate the D-pantolactone hydrolase, and the biomass of fermentation is improved. The biomass of fermentation of the culture medium is greater than or equal to 7.8 g / L dry mycelium. Compared with the domestic technology, the culture medium for producing the D-pantolactone hydrolase by fermentation through the fusarium oxysporum mycete has the advantages that the biomass of fermentation is increased by 8.3% or above; and the activity of production of the D-pantolactone hydrolase reaches 1.29 mu / g (dry mycelium) or above, and the enzyme activity is increased by 30% or above.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a medium for fermenting and producing D-pantolactone hydrolase by using fusarum oxysporm. Background technique [0002] Calcium D-pantothenate, also known as VB5, chemical name D-N-(2,4-dihydroxy-3,3-dimethylbutyryl)-β-alanine calcium), relative molecular weight: 476.54. Calcium pantothenate can be divided into three types: DL-type (mixed body), D-type (dextral body), and L-type (left-handed body), of which only the D-type (dextral body) has biological activity. Calcium D-pantothenate enters the human body to release calcium and pantothenic acid, and pantothenic acid is the precursor of coenzyme A, which is then converted into coenzyme A to produce physiological effects, participate in the metabolism of carbohydrates, fats and proteins, and maintain normal physiology for humans and animals Trace substances indispensable to functions. Mainly used in medicine, food and feed ...

AI Technical Summary

Problems solved by technology

[0006] 1 The cost of fermentation raw materials is high

In general, in the culture medium of D-pantolactone hydrolase, peptone and casein should be added as nitrogen source substances. The price of such raw materials is relatively high, resulting in increased production costs.

[0007] 2 In the traditional enzyme production medium prescription, there is a lack of suitable inducers for enzyme synthesis and enzyme production accelerators, resulting in low fermentation biomass, generally around 7.2g dry mycelium / L fermentation broth; enzyme activity is not high, generally at About 1.0U / g dry mycelium