High-yield extraction method of salicin in branches and leaves of white willows

Abstract

The invention discloses a high-yield extraction method of salicin in branches and leaves of white willows. The method includes the steps of: crushing, pre-treatment, extraction, concentration, adsorption separation, decoloration, concentration crystallization, etc. In the method, before extraction, the branches and leaves of white willows are treated through ultraviolet irradiation, gas flow pulverization, liquid nitrogen freezing and microwave de-freezing, so that the cell wall structure of the branches and leaves of white willows is effectively damaged while active components in the cells are not damaged, thus remaining the nutrients as most as possible and bringing convenience to dissolution-out of the salicin in subsequent extraction step. In the extraction method, through treatment successively carried out with low-frequency ultrasonic wave, high-frequency ultrasonic wave and medium-frequency ultrasonic wave, cell tissue of the branches and leaves of white willows is further subjected to wall disruption or deformation, so that the active components are extracted more completely and extraction rate of the salicin is greatly increased; and then a filter residue of the branches and leaves of white willows is processed in a high-voltage pulse electric field, so that dissolution-out of the salicin in the filter residue of the branches and leaves of white willows is further promoted, thereby greatly increasing the yield of the salicin.

Description

technical field [0001] The invention belongs to the field of biological extraction, and in particular relates to a high-yield extraction method for salicin in white willow branches and leaves. Background technique [0002] Salicin, the English name is D(-)-Salicin, another name is D-(-)-salicin, the molecular formula is C13H18O7, the CAS number is 138-52-3, the molecular weight is 286.28, and the melting point is 199-202℃. [0003] Salicin is white crystal; taste bitter; specific optical rotation [α] 厍-45.6° (0.6 g / 100 cm 3 Anhydrous ethanol); soluble in water, easily soluble in boiling water, insoluble in ethanol (1:90), insoluble in ether or chloroform, but soluble in alkaline solution, pyridine or glacial acetic acid. Its aqueous solution has a neutral reaction, and there is no free phenolic hydroxyl group in the molecule, which belongs to the phenolic glycoside compound. Hydrolyzed by dilute acid or amygdalin to generate glucose and salicyl alcohol. The molecular form...

AI Technical Summary

Problems solved by technology

[0007] The preparation of salicin by chemical synthesis method uses various organic intermediates, the raw materials are not easy to obtain, and some organic solvents are used, the price is high, so the overall cost is high. serious pollution

[0008] At present, the main methods for extracting salicin from white willow branches and leaves include quicklime water, methanol, and ethanol extraction methods, but the yield of salicin extracted by these methods is low, the ash content is high, and the purity is low, resulting in low product prices.