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Production method of biological ferment calcium for raising

A microbial fermentation and enzyme technology, which is applied in the production field of feed biological enzyme calcium, can solve the problems of long cycle and low yield, and achieve the effects of improving water quality, raising breeding organisms, and increasing productivity

Inactive Publication Date: 2017-12-08
HARBIN INST OF TECH AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial fermentation decomposition method has the problems of long cycle and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A production method of biological enzyme calcium for feed, which comprises the following steps:

[0021] (1) Cleaning and crushing of oyster shell powder: select high-quality oyster shells, then clean the surface soil, dust, residual shellfish, etc., then grind and pulverize, and finally form a milky white powder of 50-100 mesh through coarse powder and micro powder Solid spare.

[0022] (2) Preparation and inoculation of compound microbial culture medium: brown sugar 10 g / L, molasses 10 g / L, protein powder 1 g / L, urea 3 g / L, sodium chloride 5 g / L, shell powder 20 g / L L, inoculum size 5%, water 1 L.

[0023] (3) Cultivation and fermentation: In the liquid fermentation process, the liquid fermentation temperature is between 30-33°C, and the fermentation time is 5 days. Affected by the temperature and fermentation time, the content of calcium lactate converted from oyster shells is 75.45%, the microorganisms are about 1.08 billion / ml, and the pH is 3.95.

Embodiment 2

[0025] (1) Cleaning and crushing of oyster shell powder: select high-quality oyster shells, then clean the surface soil, dust, residual shellfish, etc., then grind and pulverize, and finally form a milky white powder of 50-100 mesh through coarse powder and micro powder Solid spare.

[0026] (2) Preparation and inoculation of compound microbial culture medium: brown sugar 20 g / L, molasses 5 g / L, protein powder 2 g / L, urea 5 g / L, sodium chloride 5 g / L, shell powder 25 g / L L, inoculum size 10%, water 25 L.

[0027] (3) Cultivation and fermentation: In the liquid fermentation process, the liquid fermentation temperature is between 33-35°C, and the fermentation time is 3 days. Affected by the temperature and fermentation time, the content of oyster shells converted into calcium lactate is 90.5%, the microorganisms are about 1.2 billion / ml, and the pH is 3.88.

Embodiment 3

[0029] Cleaning and crushing of oyster shell powder: select high-quality oyster shells, then clean the surface soil, dust, residual shellfish, etc., then grind and pulverize, and finally form a 50-100 milky white powdery solid through coarse powder and micro powder.

[0030] (2) Preparation and inoculation of compound microbial culture medium: brown sugar 15 g / L, molasses 8 g / L, protein powder 1.5 g / L, urea 3 g / L, sodium chloride 5 g / L, shell powder 25 g / L L, inoculum size 10%, water 500 L.

[0031] (3) Cultivation and fermentation: In the liquid fermentation process, the liquid fermentation temperature is between 30-35°C, and the fermentation time is 5 days. Affected by the temperature and fermentation time, the content of calcium lactate converted from oyster shells is 95%, the microorganisms are about 1.25 billion / ml, and the pH is 3.81.

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Abstract

The invention discloses a production method for producing mixed liquid of calcium lactate and beneficial microorganisms by adopting a microbial fermentation process. According to the production method, finely ground oyster shells are added in a compound bacterial liquid culture medium, and lactic acid is generated by virtue of microbial metabolism to produce a biological ferment calcium solution for raising. The production method comprises the following steps: firstly, cleaning oyster shells; coarsely grinding and finely grinding the oyster shells into 50-100 meshes; adding the finely ground oyster shells as a fermented culture medium into a compound microbial culture medium; and inoculating microorganisms , and then, carrying out fermentation for 3-5 days at a proper temperature so as to produce a liquid bacterial agent of calcium lactate and beneficial microorganisms. The biological ferment calcium produced by the production method disclosed by the invention contains a large number of beneficial microorganisms capable of improving the digestive ability of culture organisms as well as high-concentration calcium lactate. The product produced by the production method disclosed by the invention is capable of raising aquatic animals and terrestrial animals, improving the digestive ability and amount of the animals, enhancing the skeletal development of the animals, improving the immunity of the animals and reducing the occurrence of diseases, so that the product has relatively broad market prospects and popularization values.

Description

technical field [0001] The invention relates to a biotechnology for producing calcium lactate for feed by microbial fermentation, and provides a production method for biological enzyme calcium for feed containing both microorganisms and calcium lactate for livestock and poultry breeding. . Background technique [0002] Calcium lactate is an important chemical raw material, which has the characteristics of high solubility, fast dissolution rate, high bioavailability and good taste. It is mainly used in food, medicine and feed industries. In the food industry, calcium lactate, as a safe food additive, can be used as a curing agent, flavor enhancer, flavoring agent, ingredient, food softening agent, stabilizer and thickener; in the pharmaceutical industry, it is the main calcium supplement for humans and animals agent; used in toothpaste as an antibacterial agent and descaling agent in light industry. [0003] At present, there are mainly three ways to produce food-grade calc...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18
CPCA23K10/12A23K10/18
Inventor 陈雷王光玉张博淞
Owner HARBIN INST OF TECH AT WEIHAI
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