Production method of biological ferment calcium for raising
A microbial fermentation and enzyme technology, which is applied in the production field of feed biological enzyme calcium, can solve the problems of long cycle and low yield, and achieve the effects of improving water quality, raising breeding organisms, and increasing productivity
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Embodiment 1
[0020] A production method of biological enzyme calcium for feed, which comprises the following steps:
[0021] (1) Cleaning and crushing of oyster shell powder: select high-quality oyster shells, then clean the surface soil, dust, residual shellfish, etc., then grind and pulverize, and finally form a milky white powder of 50-100 mesh through coarse powder and micro powder Solid spare.
[0022] (2) Preparation and inoculation of compound microbial culture medium: brown sugar 10 g / L, molasses 10 g / L, protein powder 1 g / L, urea 3 g / L, sodium chloride 5 g / L, shell powder 20 g / L L, inoculum size 5%, water 1 L.
[0023] (3) Cultivation and fermentation: In the liquid fermentation process, the liquid fermentation temperature is between 30-33°C, and the fermentation time is 5 days. Affected by the temperature and fermentation time, the content of calcium lactate converted from oyster shells is 75.45%, the microorganisms are about 1.08 billion / ml, and the pH is 3.95.
Embodiment 2
[0025] (1) Cleaning and crushing of oyster shell powder: select high-quality oyster shells, then clean the surface soil, dust, residual shellfish, etc., then grind and pulverize, and finally form a milky white powder of 50-100 mesh through coarse powder and micro powder Solid spare.
[0026] (2) Preparation and inoculation of compound microbial culture medium: brown sugar 20 g / L, molasses 5 g / L, protein powder 2 g / L, urea 5 g / L, sodium chloride 5 g / L, shell powder 25 g / L L, inoculum size 10%, water 25 L.
[0027] (3) Cultivation and fermentation: In the liquid fermentation process, the liquid fermentation temperature is between 33-35°C, and the fermentation time is 3 days. Affected by the temperature and fermentation time, the content of oyster shells converted into calcium lactate is 90.5%, the microorganisms are about 1.2 billion / ml, and the pH is 3.88.
Embodiment 3
[0029] Cleaning and crushing of oyster shell powder: select high-quality oyster shells, then clean the surface soil, dust, residual shellfish, etc., then grind and pulverize, and finally form a 50-100 milky white powdery solid through coarse powder and micro powder.
[0030] (2) Preparation and inoculation of compound microbial culture medium: brown sugar 15 g / L, molasses 8 g / L, protein powder 1.5 g / L, urea 3 g / L, sodium chloride 5 g / L, shell powder 25 g / L L, inoculum size 10%, water 500 L.
[0031] (3) Cultivation and fermentation: In the liquid fermentation process, the liquid fermentation temperature is between 30-35°C, and the fermentation time is 5 days. Affected by the temperature and fermentation time, the content of calcium lactate converted from oyster shells is 95%, the microorganisms are about 1.25 billion / ml, and the pH is 3.81.
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