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Microbial Agents for Improving Tobacco Quality

A microbial preparation and quality technology, applied in the field of microorganisms, can solve problems such as poor ability to improve aroma and tobacco quality, unsuitable for promotion and use, and poor ability to degrade nitrite

Active Publication Date: 2021-07-06
湖北京晟生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent technology "A Composite Aroma-Producing Microorganism and Its Preparation Method and Application" discloses a microorganism that improves aroma. The microbial preparation is used in tobacco stems and cannot reduce harmful substances such as nitrite and fused-ring aromatics in tobacco. content; the patented technology "a microbial agent for tobacco and its preparation method and application" discloses a microbial agent prepared from Candida utilis and Bacillus subtilis, which has a certain ability to degrade fused-ring aromatic hydrocarbons ability, but the ability to degrade nitrite is poor, and the ability to improve aroma and tobacco quality is poor, so it is not suitable for promotion and use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Microbial formulations for improving tobacco quality, which are uniformly mixed by plant lactobacillus fermentation liquid and green xermewromewromewromewromeworr, then add 1% by weight of cellulose, 30 ° C for 12 h, then add 3 times volume The Bacillus Bacillus fermentation broth, mix well, that is, it is obtained.

[0035] The plant lactobacillus fermentation solution is prepared as follows:

[0036] Plant Lactobacillus seed fluid (1 × 10 8 CFU / mL) was transferred to the fermentation tank in a fermentation tank to obtain a fermentation tank in a fermentation tank, and the fermenter was divided into: 5 wt% glucose, 2 wt% protein, 1 wt% ammonium chloride Potassium dihydrogen phosphate 0.2% by weight, 0.05 wt% sodium acetate, 0.05 wt% magnesium sulfate, 0.01% by weight of manganese sulfate, and the remaining amount is water, pH is 6.2;

[0037] The Bacillus fermentation fluid is prepared in accordance with the following steps:

[0038] Seed liquid (1 × 10) 8 CFU / mL) was ...

Embodiment 2

[0044] A microbial formulation for improving tobacco quality, which is mixed by the plant Lactobacillus fermentation broth and the green xermewromewromewromewromewromeworr in 5: 3, then add 2 wt% cellulose, 30 ° C for 12 h, and then add 2 times volume The Bacillus Bacillus fermentation broth, mix well, that is, it is obtained.

[0045] The plant lactobacillus fermentation solution is prepared as follows:

[0046] Plant Lactobacillus seed fluid (1 × 10 8CFU / mL) was transferred to the fermentation tank in a fermentation tank to obtain a fermentation tank in a fermentation tank, and the fermenter was divided into: 5 wt% glucose, 2 wt% protein, 1 wt% ammonium chloride Potassium dihydrogen phosphate 0.2% by weight, 0.05 wt% sodium acetate, 0.05 wt% magnesium sulfate, 0.01% by weight of manganese sulfate, and the remaining amount is water, pH is 6.2;

[0047] The Bacillus fermentation fluid is prepared in accordance with the following steps:

[0048] Seed liquid (1 × 10) 8 CFU / mL) w...

Embodiment 3

[0054] Effect of microbial formulations of the present invention on harmful substances in tobacco:

[0055] Take Example 1 as an example; blank control group: non-microbial preparation treatment; Comparative Example 1: Add Plant Lactobacillus and Green Trichrus, non-additional spacialus; Comparative Example 2: Add Plant Lactobacillus and Shortensus Bacillus, do not add green woodcile; contrast 3: Add Green Trichoderma and Short Bacillus, does not add plant Lactobacillus; Comparative Example 4: Add Green Wood Musicewool. The method of treating the microbial formulation of each container is the same as in Example 1, and the tobacco sample is identical.

[0056] 1. Degradation of cellulose, pectin, and protein is shown in Table 1, the degradation rate = (contains the composition content of the ingredient content of the blank control - the composition content containing the content of the sample) / the content containing the ingredient content of the blank control.

[0057] Table 1

...

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PUM

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Abstract

The invention belongs to the technical field of microbes, and discloses a microbial preparation for improving the quality of tobacco, which is prepared according to the following process: mixing evenly the fermented liquid of Lactobacillus plantarum and the fermented liquid of Trichoderma viride, then adding cellulose, at 25-30°C Cultivate for a period of time, then add Bacillus pumilus fermentation broth, mix evenly, and obtain. The microbial preparation of the invention can improve the quality of the tobacco leaves, increase the aroma, and reduce the content of harmful substances in the tobacco, thus killing two birds with one stone.

Description

Technical field [0001] The present invention relates to the field of microbial technology, and more particularly to microorganisms for improving tobacco quality. Background technique [0002] Tobacco is a tubular flower subject, a born or limited perennial herbaceous plant, and a slightly timid of the base. Inflorescence, conical, polyhua; capsule ovate or torque round, long is equal to consotting. Summer autumn flowering results. Mainly distributed in South America, South Asia, China. It is a year or limited perennial herbs, all glandular hair; The use of tobacco is a cigarette. [0003] As we all know, long-term smoking is seriously threatening life and health. Smoking can induce pulmonary cancer and laryngeal, bronchitis, emphysema, and other respiratory diseases. Various situations caused by smoking have become a concern of globality. How to reduce the quality and aroma of the tobacco while reducing the content of tobacco, is a problem that needs to be solved in the area of ​...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A24B15/20C12R1/25C12R1/885C12R1/07
CPCA24B15/20C12N1/14C12N1/20
Inventor 鲍增明
Owner 湖北京晟生物科技股份有限公司
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