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Beauveria bassiana BB-T02 having strong infestation on hot zone flower and fruit thrips

A technology of Beauveria bassiana, BB-T02, applied in the direction of fungi, microorganism-based methods, biocides, etc., to achieve the effects of easy cultivation, maintenance of ecological balance, high spore production and spore germination rate

Active Publication Date: 2017-11-24
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Beauveria bassiana ( Beauveria spp. ) is one of the most widely studied and used entomogenic fungi in the world. It is highly pathogenic to pests and relatively safe to humans, animals, plants and the ecological environment; some strains have been screened for the prevention and control of thrips. Such as Li Yinping (2013) from the Asian corn borer ( Ostrinia furnacalis ) isolated from the carcass of the western flower thrips ( Frankliniella occidentalis ) high-efficiency Beauveria bassiana SZ-26 strain (Chinese Journal of Biological Control); Yuan Shengyong et al. Argyrogramma agnata ) larvae were isolated from the larvae of Beauveria bassiana Bb 080717 and MZ050724, which were highly virulent to Thrips occidentalis, and strains Bb 080717 and MZ050724 were also found to be highly virulent to Thrips occidentalis (Journal of Southwest University, Environmental Entomology and Chinese Forest Pests); Wang Haihong et al. (2014) from the corn borer ( Ostrinia nubilalis Beauveria bassiana GZGY-1-3 and SCWJ-2 strains (CN 104212724 A and CN 104263655 A) with good control effects on Western flower thrips were isolated on Beauveria bassiana BBNS-J9-16 strain (CN 106135295 A) with good control effect on horses, etc.; but these strains are only a small part of the Beauveria bassiana strains, there are many other hosts and geographical sources High-efficiency strains to be discovered

Method used

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  • Beauveria bassiana BB-T02 having strong infestation on hot zone flower and fruit thrips
  • Beauveria bassiana BB-T02 having strong infestation on hot zone flower and fruit thrips

Examples

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Effect test

Embodiment 1

[0014] Beauveria bassiana ( Beauveria bassiana ) Isolation and identification of BB-T02 and determination of spore production and spore germination rate

[0015] 1. Test method

[0016] 1) Isolation of strains: Pine massoniana caterpillars ( Dendrolimus punctatus ) larvae, use a sterilized scalpel to scrape the spore powder on the surface of the worm body into 10 mL of sterile water, filter the spore stock solution through degreasing gauze, and then spread the spore stock solution evenly on the PDA plate medium, and then place it at a temperature (25 ±1)°C, relative humidity (90±3)%, and photoperiod L:D=10:14 in a light incubator for 8 days.

[0017] 2) Morphological identification: After the isolated strains were cultured for 8 days, first observe the characteristics of the colony, and then use an inverted microscope to observe the morphological characteristics of the strain's hyphae, conidiophores, spore-forming cells, and conidia.

[0018] 3) Molecular biology identifi...

Embodiment 2

[0028] Beauveria bassiana ( Beauveria bassiana ) BB-T02 on the Ficus mother tube thrips ( Gynaikothrips ficorum ) and Banana flower thrips ( Thrips hawaiiensis ) Infestation and lethal effect of thrips in isothermal zone

[0029] 1, test method: get (embodiment 1) isolated Beauveria bassiana ( Beauveria bassiana ) BB-T02, inoculated on PDA plate medium, placed in a light incubator at temperature (25±1)°C, relative humidity (90±3)%, and photoperiod L:D=10:14 for 8 days; Add 10 mL of sterile water containing 0.05% Tween-80 to the petri dish to delyze the spores, filter through three layers of degreasing gauze to obtain the spore stock solution; use a Neubauer hemocytometer to count the number of spores in the spore stock solution under a biological microscope and count the spores Concentration, and then dilute the spore stock solution with sterile water to 1.00×10 4 , 1.00×10 5 , 1.00×10 6 , 1.00×10 7 and 1.00×10 8 5 concentrations of spores / mL, 0.05% Tween-80 sterile w...

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PUM

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Abstract

The invention relates to beauveria bassiana BB-T02 having strong infestation on hot zone flower and fruit thrips and belongs to the technical field of biological prevention and control. A preservation number of the beauveria bassinan BB-T02 is CGMCC No.14130. The beauveria bassinan has easiness in culturing, high sporulation quantity and spore germination rate, good infestation lethal effects on the hot zone flower and fruit thrips, relative safety to human, livestock, plant and ecological environment, potency in replacing a part of chemical thrip prevention pesticides and benefit of maintaining ecological balance.

Description

technical field [0001] The invention relates to a strain of Beauveria bassiana BB-T02 which has strong infectivity to flower and fruit thrips in hot zone, and belongs to the technical field of biological control. Background technique [0002] Thrips is a general term for insects of the order Thysanoptera. Thrips have miscellaneous feeding habits, and herbivorous species are more common. They are small in size or covert in activities. It is difficult for people to notice the damage in the early stage, and it is often discovered after causing serious disasters. In recent years, The occurrence of this insect in the tropics and subtropical regions (referred to as the hot zone) is increasing, and it has become one of the main pests on the flowers and fruits in the tropical zone such as banyan trees, orchids, bananas and mangoes. Thrips scrapes the epidermis of plants with its file-suction mouthparts, inserts its stylet into the tissue to absorb juice, and likes to eat young parts...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 黄鹏余德亿姚锦爱
Owner INST OF PLANT PROTECTION FAAS
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