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Extraction and transformation coupling technique for preparing ginsenoside composition

A technology of ginsenoside and composition, which is applied in the field of preparation of traditional Chinese medicine medicinal composition compositions, can solve problems such as the ginsenoside composition that has not been found yet, and achieve the effect of high yield

Inactive Publication Date: 2017-11-24
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No literature reports have been found on the preparation of ginsenoside compositions, especially high-content ginsenoside Rd compositions, by coupling enzyme extraction and transformation techniques

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 50 mg of American ginseng medicinal material powder and add it to a 25ml stoppered test tube, add 24 times the amount of enzyme solution, adjust the volume of the extract with water, adjust the pH to 2 with hydrochloric acid, and carry out enzymatic hydrolysis at a temperature of 40°C. After 60 minutes of hydrolysis, Take out the stoppered test tube, pour out the supernatant, add 12 times of water to the residue, perform a second extraction with the same extraction method, and combine the two extracts. The pH of the extract was adjusted to 6.5 with ammonia water, and the flow rate of sample loading and cleaning was controlled at 0.5mL min -1 , add the supernatant to the pretreated D101 resin column (1.5×25cm glass column), wash with 20% ethanol, elute with 50% ethanol, concentrate the aqueous ethanol eluent under reduced pressure and dry it. Sonicate with 95% ethanol and dilute to 10ml. Filter through a 0.45µm filter head, inject 10µl of sample, and measure by HPL...

Embodiment 2

[0016] Take 500 mg of American ginseng medicinal material powder and add it to a 25ml stoppered test tube, add 24 times the amount of enzyme solution, adjust the volume of the extract with water, adjust the pH to 4 with hydrochloric acid, and carry out enzymatic hydrolysis at a temperature of 40°C. After 120 minutes of hydrolysis, Take out the stoppered test tube, pour out the supernatant, add 18 times of water to the residue, perform a second extraction with the same extraction method, and combine the two extracts. The pH of the extract was adjusted to 6.5 with ammonia water, and the flow rate of sample loading and cleaning was controlled at 1mL min -1 , add the supernatant to the pretreated D101 resin column (1.5×25cm glass column), elute with 50% ethanol, concentrate the aqueous ethanol eluate under reduced pressure and then dry it. Take an appropriate amount of dry matter and dissolve it ultrasonically with 95% ethanol and dilute to 1ml. Filter through a 0.45µm filter hea...

Embodiment 3

[0018] Take 500 mg of American ginseng medicinal powder and add it to a 25ml stoppered test tube, add pectinase with 10% of the medicinal material, add water 24 times the medicinal material, adjust the pH to 6 with hydrochloric acid, and control the temperature at 40°C for enzymatic hydrolysis and hydrolysis. After 180 minutes, take out the stoppered test tube, pour out the supernatant, add 24 times of water to the residue, perform a second extraction with the same extraction method, and combine the two extracts. Adjust the pH of the extract to 6.5 with ammonia water, and control the flow rate of sample loading and cleaning to 2mL min -1 , add the supernatant to the pretreated D101 resin column (1.5×25cm glass column), elute with 50% ethanol, concentrate the aqueous ethanol eluate under reduced pressure and then dry it. Take an appropriate amount of dry matter and dissolve it ultrasonically with 95% ethanol and dilute to 1ml. Filter through a 0.45µm filter head, inject 10µl o...

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Abstract

The invention relates to a preparation method for a traditional Chinese medicinal ingredient composition, and in particular to an extraction and transformation coupling technique for extracting, preparing and separating a ginsenoside composition from ginseng and American ginseng materials. The method includes the following steps: fine ginseng and American ginseng material powder (screened by 40 meshes) is chosen and added with an enzyme solution which is 12 to 24 times in amount, pH is regulated to acid by diluted hydrochloric acid, and ultrasonic extraction is carried out under a certain temperature. The pH of hydrolysate is regulated to 6.5 to 7.0 by ammonia, flow velocity is controlled, the supernate is added onto a pretreated macroporous resin column, 0 to 30 percent of ethanol is used for washing, 50 to 80 percent of ethanol is used for elution, the eluent is concentrated and dried, the obtained dried material contains 3 to 5 percent of ginsenoside Rg1, 20 to 40 percent of ginsenoside Re, 0 to 10 percent of ginsenoside Rb1 and 0 to 70 percent of ginsenoside Rd4, and the composition of the ginsenoside composition is different from the existing products, such as total ginsenoside, panax notoginseng saponins and notoginseng leaf triterpenes. The preparation method disclosed by the invention is simple and convenient, extraction and transformation are synchronized, the cost is low, yield is high, and the preparation method is suitable for the mass preparation of the ginsenoside composition, particularly high-content ginsenoside Rd composition.

Description

technical field [0001] The invention relates to a preparation method of a traditional Chinese medicine active ingredient composition, in particular to an extraction and transformation coupling technology for preparing and separating a ginsenoside composition from ginseng and American ginseng medicinal materials. Background technique [0002] Panax ginseng and American ginseng are precious Chinese medicinal materials, which have various functions such as anti-cancer, anti-fatigue, anti-hypoxia, anti-radiation, and anti-aging. curative effect. Ginsenosides are the main medicinal ingredients of Panax genus medicinal materials. It has been reported in the literature that more than 70 species of ginsenosides have been isolated and their structures determined, and most of the saponin monomers have significant pharmacological effects and medicinal value. According to the structure of saponins, ginsenosides are divided into protopanaxadiol type saponins (Rb1, Rb2, Rb3, Rc, Rd, Rh2,...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 房海燕
Owner QILU UNIV OF TECH
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