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Chitosan-based periodontal local drug slow-release hydrogel and application thereof

A topical drug and hydrogel technology, which is applied in drug combinations, medical preparations containing active ingredients, and pharmaceutical formulas, to achieve the effects of low risk, simple experimental operation, and good experimental repeatability

Inactive Publication Date: 2017-11-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The key to the treatment of periodontitis, on the one hand, is to inhibit periodontal inflammation; on the other hand, it is to promote the regeneration of periodontal tissue, but the existing treatment methods are difficult to achieve the above requirements at the same time

Method used

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  • Chitosan-based periodontal local drug slow-release hydrogel and application thereof
  • Chitosan-based periodontal local drug slow-release hydrogel and application thereof
  • Chitosan-based periodontal local drug slow-release hydrogel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of each component solution of the hydrogel: 20 mg of aspirin (US Sigma Company) was added to 20 mL of 0.1 mol / L hydrochloric acid (Beijing Chemical Plant) solution, and 0.4 g of chitosan (US Sigma Company) was added. ), stir to obtain aspirin-containing chitosan solution. 1 g of sodium β-glycerophosphate (Sigma, USA) was added to 2 mL of deionized water, and 6000 U of EPO (China Sansheng Pharmaceutical Co., Ltd.) was added, and stirred evenly to obtain a sodium β-glycerophosphate solution containing EPO. Add 20 mg of gelatin (Sigma, USA) into 10 mL of deionized water, and stir evenly to obtain a gelatin solution.

[0018] (2) Gelation of chitosan thermosensitive hydrogel loaded with aspirin and EPO: take 2mL of the above-mentioned chitosan solution containing aspirin, add 0.25mL of the above-mentioned β-glycerol dropwise at a volume ratio of 8:1:0.25 Sodium phosphate solution and 62.5 μL of the above-mentioned gelatin solution were stirred evenly, place...

Embodiment 2

[0021] (1) Preparation of the solutions of the various components of the hydrogel: 40 mg of aspirin was added to 40 mL of 0.1 mol / L hydrochloric acid solution, and 0.8 g of chitosan was added, and stirred evenly to obtain a chitosan solution containing aspirin. Add 2 g of sodium β-glycerophosphate to 4 mL of deionized water, and add 10000 U of EPO, and stir evenly to obtain a solution of sodium β-glycerophosphate containing EPO. Add 100 mg of gelatin into 20 mL of deionized water, and stir evenly to obtain a gelatin solution.

[0022] (2) Gelation of chitosan thermosensitive hydrogel loaded with aspirin and EPO: Take 4 mL of the above chitosan solution, add 1 mL of the above β-glycerophosphate dropwise at a volume ratio of 12:3:1 Solution and 333 μL of the above-mentioned gelatin solution were stirred evenly, and placed in constant temperature water baths at 33°C, 34°C, 35°C, 36°C, 37°C, 38°C and 40°C respectively, and their gelatinization at different temperatures were detect...

Embodiment 3

[0024] (1) Preparation of the solution of each component of the hydrogel: 30 mg of aspirin was added to 30 mL of 0.1 mol / L hydrochloric acid solution, and 0.5 g of chitosan was added, and stirred evenly to obtain a chitosan solution containing aspirin. Add 1.5 g of β-sodium glycerophosphate to 3 mL of deionized water, and add 8000 U of EPO, and stir evenly to obtain a solution of β-sodium glycerophosphate containing EPO. Add 50 mg of gelatin into 15 mL of deionized water, and stir evenly to obtain a gelatin solution.

[0025](2) Gelation of chitosan temperature-sensitive hydrogel loaded with aspirin and EPO: Take 3mL of the above-mentioned chitosan solution, add 0.6mL of the above-mentioned β-glycerophosphate dropwise at a volume ratio of 10:2:0.5 Sodium solution and 150 μL of the above gelatin solution, stir well.

[0026] (3) In order to investigate the control effect of the aspirin and EPO-loaded chitosan thermosensitive hydrogel on periodontal inflammation and the effect ...

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Abstract

The invention relates to chitosan-based periodontal local drug slow-release hydrogel and application thereof, belonging to the technical field of preparation of local drug slow-release carriers. The invention in particular relates to chitosan temperature-sensitive hydrogel loading aspirin and erythrogenin (EPO), and the chitosan temperature-sensitive hydrogel is obtained by mixing an aspirin-containing chitosan solution, an EPO-containing beta-sodium glycero-phosphate solution and a gelatin solution according to a proportion at the temperature of 34-40 DEG C. The surface pore size of the chitosan temperature-sensitive hydrogel loading the aspirin and the EPO prepared by the method is about 40-70mu m and can be gelatinized at the temperature of 34-40 DEG C; in a rat maxillary first molar periodontitis model constructed by using ligature wires, the effects of controlling periodontal inflammation and promoting periodontal tissue regeneration can be achieved by locally injecting the hydrogel. Therefore, the chitosan-sodium glycerophosphate-gelatin injectable temperature-sensitive hydrogel loading the aspirin and the EPO has better application value and prospects when being used as a local drug slow-release carrier for periodontitis treatment.

Description

technical field [0001] The invention belongs to the technical field of preparation of local drug slow-release carriers, and in particular relates to a chitosan-based temperature-sensitive hydrogel, which is compounded with drugs aspirin and erythropoietin (EPO) and locally applied to teeth. The periodontal model can not only control periodontal inflammation, but also promote periodontal tissue regeneration, so it can be used as a periodontal local drug sustained release system and applied to drug treatment after periodontal basic treatment. Background technique [0002] Periodontitis is an inflammatory disease caused by the complex interaction between plaque microorganisms and the host immune system. Alveolar bone resorption caused by periodontitis is the first cause of tooth loss in adults. Studies have shown that periodontitis not only causes tooth loss, but also affects the health of the whole system. As a pathogenic bacteria of periodontitis, porphyromonas gingivalis (P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/42A61K38/19A61P1/02A61K31/616
Inventor 徐晓薇孙宏晨张恺赵亮申玉芹顾中一何居洋林崇韬杨柏
Owner JILIN UNIV
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