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Gene marker, kit and method for detecting lung cancer

A technology for gene markers and lung cancer, which is applied in the field of gene markers for detection of lung cancer, can solve the problems of low sensitivity and specificity, difficulty in widespread and routine application, and difficulty in guaranteeing accuracy in early lung cancer, so as to achieve easy recurrence and metastasis, Easy dynamic monitoring, high accuracy effect

Pending Publication Date: 2017-11-21
SHANGHAI EPICAN BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, imaging is easily affected by operator experience, and depends on equipment, which is expensive, especially in the case of limited medical resources, its accuracy is difficult to guarantee, and it is difficult to be widely and routinely applied
Tissue biopsy is currently the gold standard for clinically diagnosing lung cancer. However, tissue biopsy has major limitations, such as difficulty in surgical sampling, or inconvenient puncture at certain cancer sites, and puncture itself will also bring certain clinical risks. Repeated puncture screening Checking will cause great pain to patients
The most widely used serological test is the detection of carcinoembryonic antigen (CEA), but the sensitivity and specificity of CEA for early lung cancer are not high

Method used

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  • Gene marker, kit and method for detecting lung cancer
  • Gene marker, kit and method for detecting lung cancer
  • Gene marker, kit and method for detecting lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Screening of lung cancer gene markers

[0027] 1) Extract plasma DNA:

[0028] 10 ng of plasma DNA was extracted from the samples of 20 lung cancer patients and 20 normal persons, and this step could be performed using any methods and reagents known to those skilled in the art that are suitable for extracting plasma DNA.

[0029] 2) End-fill the plasma DNA, overhang A and connect with the sequencing adapter:

[0030] Prepare a reaction mixture containing 50uL plasma DNA, 7uL End Repair & A-Tailing Buffer and 3uL End Repair & A-Tailing Enzyme mix according to the Kapa Hyper Perp Kit instructions (the total volume is 60uL), incubate at 20°C for 30 minutes, and then incubate Incubate at 65°C for 30 minutes. Prepare the following ligation reaction mixture in a 1.5mL low adsorption EP tube: 5uL Nuclease free water, 30uL Ligation Buffer and 10 uL DNA Ligase. Add 5uL of sequencing adapters to 45uL ligation reaction mixture, mix, heat at 20°C for 20 minutes, then ...

Embodiment 2

[0047] Example 2. Effectiveness of Gene Markers for Lung Cancer

[0048] This example verifies the effectiveness of the lung cancer gene markers of the present invention for detecting lung cancer.

[0049] According to the method of Example 1, the 5-hmC content of the 10 lung cancer gene markers described in the present invention in the first batch of 80 samples (40 cases of lung cancer and 40 cases of healthy controls) was determined.

[0050] The standardized 5-hmC content of each gene marker is multiplied by the corresponding weighting coefficient of the marker in Example 1 to obtain the predictor t of the gene marker, and then the predictor t of each gene marker is added to obtain The total predictor T, and then the total predictor T is transformed according to the following formula to obtain the score P:

[0051]

[0052] If P>0.5, the subject sample has lung cancer; if P≤0.5, the subject sample is normal.

[0053] figure 1 The results of differentiating the batch o...

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Abstract

The invention relates to a gene marker, kit and method for detecting lung cancer. The gene marker comprises one or more than two of a RUNX1 translocation partner gene 1, F-Box and leucine-rich repeat protein 7, RNcA binding motif single chain interacting protein 3, cadherin 11, erythrocyte membrane protein band 4.1-like protein, basonuclin 2, Tolloid-like protein 1, sulfatase 1, integrin subunit alpha 8 and R-spinal protein 3. The content of 5-hydroxymethyl cytosine in the lung cancer gene marker through high-throughput sequencing so as to judge whether the lung cancer exists or not. The method for detecting the lung cancer is safe, noninvasive, wide in DNA source, high in accuracy, convenient to operate and good in user experience. The gene marker can be combined with other clinical indexes and provides accurate judgements for lung cancer screening, diagnosing, treatment and prognosis.

Description

technical field [0001] The invention relates to the technical field of clinical molecular diagnosis of lung cancer. Specifically, the present invention relates to gene markers, kits and methods for detecting lung cancer. Background technique [0002] Primary bronchial lung cancer (abbreviated as lung cancer) is one of the most common malignant tumors in the world at present. The World Health Organization International Cancer Research Center (IARC / WHO) reported that the global male incidence rate of lung cancer in 2002 was 35.5 / 100,000, and the incidence rate was 97. Ten thousand people, the mortality rate was 31.2 / 100,000, and 850,000 died; the incidence rate of women was 12.1 / 100,000, and the incidence was 390,000, the mortality rate was 10.3 / 100,000, and 330,000 died. According to the "Third National Survey on Causes of Death among Residents" released by the Ministry of Health of my country in April 2008, the incidence of lung cancer in my country has increased significan...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6886C12Q2600/154C12Q2535/122
Inventor 陆星宇宋艳群彭莱张子谋
Owner SHANGHAI EPICAN BIOTECH CO LTD
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